Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2011.394.398Bacillus megaterium Strain GS1 Isolated from Volcanic Area Gunung Sibayak]]>Md. SalimMashitah D.D. Roslan HuyopF. 52011115A bacterial species identified as Bacillus megaterium GS1 was previously isolated from volcanic area of Gunung Sibayak, Indonesia. Therefore, the main aim of the present study was to identify the presence of dehalogenase gene in the microorganism. To achieve this, is to apply basic molecular techniques that include the use of oligonucleotide primers specific to microorganism that can grow in halogenated compound. A putative dehalogenase gene was determined by direct sequencing and analysis of the PCR-amplified genomic DNA of the bacterium. A comparative analysis of the sequence data revealed that, DehGS1 amino acid sequence is related to L-specific dehalogenase or group II αHA with an overall of 25% amino acids identity. This investigation is useful in studying the microbial populations in order to monitor the presence of specific or novel type dehalogenase genes. As a result, it will provide better understanding of the microbial populations that present in soil or in water systems treating halogenated compounds.]]>Cairns, S.S., A. Cornish and R.A. Cooper,1996Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity.]]>235744749Fetzner, S.,199850633657Fetzner, S. and F. Lingens,199458641685Fortin, N., R.R. Fulthorpe, D.G. Allen and C.W. Gree,199844537546Hareland, W.A., R.L. Crawford, P.J. Chapman and S. Dagley,1975Pseudomonas acidovorans.]]>121272285Huang, J., Y. Xin and W. Zhang,2011Paracoccus sp. 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis.]]>51318324Ismail, S.N., A.M. Taha, N.H. Jing, R.A. Wahab, A.A. Hamid, R.V. Pakingking Jr. and F. Huyop,2008Pseudomonas sp. strain R1 bacterium isolated from Malaysian paddy (rice) field.]]>7481486Janssen, D.B., F. Pries and J. van der Ploeg,199448163191Jing, N.H., A.M. Taha, R.V. Pakingking, Jr., R.A.B. Wahab and F. Huyop,2008Methylobacterium sp. HJ1 induced by the herbicide 2,2-dichloropropionate (Dalapon).]]>23236Jing, N.H. and F. Huyop,2008Methylobacterium sp. HJ1.]]>8233235Jones, D.H.A., P.T. Bath, D. Byrom and C.L. Thomas,1992Pseudomonas putida strain AJ1 and purification of the encoded protein.]]>138675683Kawasaki, H., T. Toyama, T. Maeda, H. Nishino and K. Tonomura,1994Pseudomonas putida no. 109.]]>58160163Kurihara, T.,201175189198Leigh, J.A.,19861986Mesri, S., R.A. Wahab and F. Huyop,2009Pseudomonas sp. B6P isolated from a rice paddy field.]]>59447451Murdiyatmo, U., W. Asmara, J.S.H. Tsang, A.J. Baines, A.T. Bull and D.J. Hardman,1992Pseudomonas cepacia MBA4.]]>2848793Slater, J.H., A.T. Bull, D.J. Hardman,19956181189Stringfellow, J.M., S.S. Cairns, A. Cornish and R.A. Cooper,1997Rhizobium sp.-molecular analysis of the gene and formation of carbon monoxide from trihaloacetate by the enzyme.]]>250789793Swanson, P.E.,199910365369Thasif, S., S. Hamdan and F. Huyop,20098264269Thomas, A.W., J.H. Slater and A.J. Weightman,1992Pseudomonas putida PP3 is carried on unusual mobile genetic element designated DEH.]]>17419321940Van der Ploeg, J., G. van Hall and D.B. Janssen,1991Xanthobacter autotrophicus GJ10 and sequencing of the dhlb gene.]]>17379257933Wu, S.J., H.X. Zhang, Z.H. Hu and J.M. Chen,2009Bacillus circulans WZ-12.]]>3024792484Yusn, T.Y. and F. Huyop,2009Escherichia coli JM109 expressing dehalogenase (deh) gene used as selection marker.]]>8385388Zulkifly, A.H., D. Roslan, A.A.A. Hamid, S. Hamdan and F. Huyop,2010Bacillus sp. TW1 isolated from terengganu water treatment and distribution plant.]]>1029402944Hill, K.E., J.R. Marchesi and A.J. Weightman,199918125352547