Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2011.299.306Bacillus sp. Gs-3]]>George-OkaforU. O.OdiboF. J.C.42011114There are limited reliable information on the commercial production and utilization of proteases for detergent and other industrial uses in Nigeria. Hence, the purification and characterization of Bacillus sp. Gs-3 protease for its potential industrial uses were investigated. The dialyzed crude enzyme was purified 17-fold in a two-step procedure involving carboxymethyl sepharose ionic-exchange chromatography and phenyl sepharose 6-fast flow hydrophobic interaction chromatography. The purified enzyme had its optimal activity at pH 9.0 and 90°C and was stable over a pH range 8.0-11.0. It readily hydrolyzed all the tested protein substrates but exhibited highest affinity for gelatin (Km 0.15 mg mL-1). It retained at least 66.2±0.02% of its original activity in the presence of the tested local commercial detergents and removed bloodstains completely. Its activity was significantly (p>0.05) enhanced by Cu2+ ion but strongly inhibited (75.6±0.07%) by Phenyl-methyl Sulfonyl Fluoride (PMSF). Thus, the enzyme demonstrated desirable properties suitable for its biotechnological applications especially in detergent industry.]]>Adinarayana, K., P. Ellaiah and D.S. Prasad,2003Bacillus subtilis PE-11.]]>419Aftab, S., S. Ahmed, S. Saeed and S.A. Rasool,2006921222126Habib, S.M.A., A.N.M. Fakhruddin, S. Begum and M.M. Ahmed,2011Halobacterium sp. AF1.]]>3345356Al-Shehri, M.A. and M.S. Yasser,2004Bacillus licheniformis isolated from Tihamet Aseer, Saudi Arabia.]]>716311635Anwar, A. and M. Saleemuddin,2000Spilosoma obliqua: Potential applications in bioformulation.]]>318589Bradford, M.M.,197672248254Donaghy, J.A. and A.M. McKay,1993Aureobasidium pullulans.]]>74662666Durham, D.R.,198763381386Durham, D.R., D.B. Stewart and E.J. Stellwag,1987Bacillus species strain GX 6638.]]>16927622768Gimenez, M.I., C.A. Studdert, J.J. Sanchez and R.E. De Castro,2000Natrialba Magadii purification and biochemical characterization.]]>4181188Griffin, H.L., R.V. Greene and M.A. Cotta,199224111117Hameed, A., T. Keshavarz and C.S. Evans,1999Bacillus subtilis K2, for use in leather processing.]]>7458Ito, S., T. Kobayashi, K. Ara, K. Ozaki, S. Kawai and Y. Hatada,19982185190Khan, H., M. Subhan, S. Mehmood, M.F. Durrani, S. Abbas and S. Khan,2008Holarrhena antidysenterica.]]>79499Khan, A.R., S. Nirasawa, S. Kaneko, T. Shimonishi and K. Hayashi,2000Aquifex aeolicus.]]>278388Kumar, D., Savitri, N. Thakur, R. Verma and T.C. Bhalla,20083661672Lineweaver, H. and D. Burk,193456658666Odibo, F.J.C. and S.K.C. Obi,1988Thermoactinomyces thalpophilus.]]>4327332Odibo, F.J.C. and R. Ulbrich-Hofmann,2001Thermomyces lanuginosus F1.]]>21141153Ogbonna, A.C., S.K.C. Obi, B.N. Okolo and F.J.C. Odibo,2003109179186Ogbonna, A.C., S.K.C. Obi, B.N. Okolo and F.J.C. Odibo,200484113120Outtrup, H. and C.O.L. Boyce,19901990pp: 227-254pp: 227-254Pawar, R., V. Zambare, S. Barve and G. Paratkar,2009Bacillus sp. 158 in enzymatic cleansing of contact lenses.]]>8276280Plummer, D.T.,19781978pp: 243-287pp: 243-287Rahman, R.N.Z.A., C.N. Razak, K. Ampon, M. Basri, W.M. Zin, W. Yunus and A.B. Salleh,1994Bacillus stearothermophilus F1.]]>40822827Tork, S., M.M. Aly and L. Nawar,2010Pseudomomanas sp., MS21.]]>2113Tsuchida, O., Y. Yamagata, T. Ishizuka, T. Arai, J.I. Yamada, M. Takeuchi and E. Ichishima,1986Bacillus sp.]]>14712Tsuchiya, K., H. Sakashita, Y. Nakamura and T. Kimura,1991Thermoactinomyces sp.]]>5531253127Upton, M.E. and W.M. Fogarty,1977Thermomonospora viridis.]]>335964Varela, H., M.D. Ferrari, L. Belobradjie, A. Vazquez and M.L. Loperena,1997Bacillus subtilis: Production and partial characterization.]]>19755758Wolff, A.M., M.S. Showell, M.G. Venegas, B.L. Barnett and W.C. Wertz,19961996pp: 113-120pp: 113-120Yamagata, Y. and E. Ichishima,1989Bacillus species.]]>19252264Hou, Y. and X.H. Zhao,201110190196Yum, R.Y., H.C. Chung, D.H. Bal, D.H. Ohi and U.H. Yu,1994Streptomyces sp. YSA-130.]]>58470474Zeikus, J.G., C. Veille and A. Savchenko,19982179183Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Showell, M.S.,19991999pp: 958-971pp: 958-971Gold, A.M. and D. Fahrney,19643783791Gupta, R., Q. Beg and P. Lorenz,2002591532