Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2010.261.266MirmomeniM.H. Sajjadi MajdS. SisakhtnezhadS. DoranegardF. 32010103The aim of this study was to compare three different techniques for optimizing DNA extraction from Paraffin-Embedded Tissues (PETs) in order to generate DNA suitable for PCR. Paraffin-embedded tissues are common resources for molecular genetic studies but the extraction of high quality nucleic acid from them is problematic. DNA extraction methods used include modified phenol-chloroform protocol, salting out method using ammonium acetate and commercial kit (QIA amp DNA Mini Kit (50)-QIAGEN). DNA was extracted using these methods and qualified using spectrophotometer analysis, electrophoresis and PCR amplification. The tissue specimens used for detection of p53 and Nat2 genes consist of liver tissues with hepatocellular carcinoma (HCC) and stomach tissues which showed stomach adenocarcinoma. DNA was extracted using the aforementioned methods. Subsequently, PCR amplification for p53 and Nat2 genes was performed and 196 and 783 bp fragments were yielded for p53 and Nat2 genes, respectively. Finally, comparison of the electrophoresis pattern in all three methods indicated that no significant differences were detected and all three methods showed equally efficient results.]]>Agundez, J.A.,20089520531Ambrosone, C.B., S. Kropp, J. Yang, S. Yao, P.G. Shields and J. Chang-Claude,2008Acetyltransferase 2 genotypes and breast cancer risk: Pooled analysis and meta-analysis.]]>171526An, S.F. and K.A. Fleming,199144924927Aplenc, R., C. Orudjev, J. Swoyer, B. Manke and T. Rebbeck,20021618651866Cao, W., M. Hashibe, J.Y. Rao, H. Morgenstern and Z.F. Zhang,200327397404Chan, P.K.S., D.P.C. Chan, K.F. To, M.Y. Yu, J.L.K. Cheung and A.F. Cheng,200154401403Chen, W.N., C.J. Oon and M.C. Moh,200020263267Coates, P.J., A.J. d'Ardenne, G. Khan, H.O. Kangro and G. Slavin,199144115118Coura, R., J.C. Prolla, L. Meurer and P. Ashton-Prolla,200558894895Diller, L., J. Kassel, C.E. Nelson, M.A. Gryka and G. Litwak et al.,19901057725781Fan, H. and L.M. Gulley,20014914Garrcia, C.A., A. Ahmadian, B. Gharizadeh, J. Lundeberg, M. Ronaghi and P. Nyren,2000253249257Gilbert, M.T.P., T. Haselkorn, M. Bunce, J.J. Sanchez and S.B. Lucas et al.,20072e537e537Gillio-Tos, A., L. de Marco, V. Fiano, F. Garcia-Bragado, R. Dikshit, P. Boffetta and F. Merletti,200739345348Hein, D.W.,20062516491658Kern, S.E., J.A. Pietenpol, S. Thiagalingam, A. Seymour, K.W. Kinzler and B. Vogelstein,1992256827830Liu, H., Y. Wang, Q. Zhou, S.Y. Gui and X. Li,20028480482Llovet, J.M. and M. Beaugrand,200338S136S149Lu, X., G. Magrane, C. Yin, D.N. Louis, J. Gray and T.V. Dyke,20012160176030Noguera, N.I., C.E. Tallano, I.M. Bragos and A.C. Milani,200114280283Pinto, A.P. and L.L. Villa,1998514849Poinar, H.N. and B.A. Stankiewikz,19999684268431Poljak, M., K. Seme and N. Gale,20004394244Rezaii, J., H. Ashegh, M. Hasibi, M. Behzadi, F. Khodadadi and M. Heidarali,20088564568Rivero, E.R.C., A.C. Neves, M.G. Silva-Valenzuela, S.O.M. Sousa and F.D. Nunes,2006202523529Santos, S., D. Sa, E. Bastos, H. Guedes-Pinto, I. Gut, F. Gartner and R. Chaves,200986421426Satiroglu-Tufan, N.L., F. Bir and E. Duzcan,200413338Sepp, R., I. Szabo, H. Uda and H. Sakamoto,199447318323Sjoholm, M.I.L., G. Hoffmann, S. Lindgren, J. Dillner and J. Carlson,200514251255Vollzenandt, M., A.P. Dicker, R. Fanin, D. Banerjee, A. Albino and J.R. Bertino,1993158088Walraven, J.M., Y. Zang, J.O. Trent and D.W. Hein,20089471486