Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.878.883Lemus-FloresC. Mejia-MartinezK. Rodriguez-CarpenaJ.G. Barreras-SerranoA. Herrera-HaroJ.G. Alonso-MoralesR. 8200998The ESR, RBP4 and FUT1 candidate genes were studied
in 103 pigs from the commercial breed Yorkshire and Mexican Creole, Pelón
and Cuino animals, for establishing genic and genotypic differences, unbiased
diversity and genetic distances by employing standard distances and phylogenetic
tree build up by the Neighbor-Joining technique. Polymorphism was determined
by PCR-RFLP methods using established procedures from other investigations.
For ESR gene there were no homozygote BB animals in Pelón and Cuino;
the allele B and A were more frequent in Yorkshire (0.38) and Cuino (0.84).
Genetic distance was greater in Yorkshire and Cuino as compared to Pelón,
with greatest heterozygocity for Yorkshire (0.479) followed by Pelón
(0.390) and Cuino (0.275). The BB genotype for RBP4 gene was not detected
in any breed examined; there was no dependence of genic and genotypic
frequencies in any breed. Allele A frequency was similar in Yorkshire,
Pelón and Cuino pigs (0.74, 0.74 and 0.75), in similitude of frequency
of AA and AB genotypes in all breeds. Genetic distances and heterozygocity
were similar in the three breeds (Yorkshire, 0.390; Pelón, 0.390
and Cuino, 0.382). For FUT1 gene a major frequency of allele G and genotype
GG was found in Yorkshire (0.60 and 0.45) and Pelón (0.64 and 0.39)
animals. The allele A was more frequent in Cuino pigs (0.55). There was
a greater genetic distance between Yorkshire and Cuino animals. Cuino
pigs showing a greater heterozygocity (0.503) as compared to Pelón
(0.465) and Yorkshire (0.448) which in turn were no different between
them.]]>Benitez, O.W. and D.M. Sanchez, 2001pp: 13-35pp: 13-35Canul, S.M., V.A. Sierra, M.A. Martinez, O.J. Ortiz, J.V. Delgado, J.L. Vega-Pla and G.F. Perez, 200554267272Chen, K.F., L.S. Huang, N. Li, Q. Zhang, M. Luo and C.X. Wu, 200027853857Ciobanu, C.D., E.A. Day, A. Nagy, R. Wales, M.F. Rothschild and S.G. Plastow, 200133417432Dekkers, J.C.,200482E313E318Drogemuller, C., H. Hamann and O. Distl,20017925652570FAO.,20112011Felsenstein, J., 19971997Goliasova, E. and J. Wolf,200435 293297Hernandez, L.S.H., C. Lemus, M.R. Alonso and H.J.G. Herrera, 200616648654Horak, P., T. Urban and J. Dvolak, 2005122210213Horogh, G., A. Zsolnai, I. Komlosi, A. Nyiri, I. Anton and L. Fesus, 20051225661Hurtado, E.,2006132629Isler, B.J., K.M. Invin, S.M. Neal, S.J. Moeller and M.E. Davis, 20028023342339Lemus, C., R. Ulloa-Arvizu, M. Ramoskuri, F.J. Estrada and R.A. Alonso, 20017930213026Lemus, C., M.R. Alonso, M. Alonso-Spilbury and N.R. Ramirez, 200352109112Lemus, C. and M.L. Alonso, 2005Linville, R.C., D. Pomp, R.K. Johnson and M.F. Rothschild,2001796067Martinez, A.M., J.V. Delgado, A. Rodero and J.L. Vega-Pla, 200031295301Meijerink, E., R. Fries, P. Vogeli, J. Masabanda and G. Wigger et al.,1997Escherichia coli F18 receptor (ECF18R) loci. ]]>8736741Mejia-Martinez, K., C. Lemus-Flores, J.F. Zambrano-Zaragoza, R. Ramirez-Necoechea, D. Mota-Rojas and M. Alonso-Spilsbury, 20087625629Messer, L.A., L. Wang, J. Yelich, D. Pomp, R.D. Geisert and M.F. Rothschild,19967396396Munoz, M., C. Ovilo, G. Munoz, A. Fernandez, E. Alves and L. Silio,20091911Nei, M.,1972106283292Nei, M.,197889583590Nei, M., F. Tajima and Y. Tateno,198319153170Noguera, J.L., L. Varona, R.L. Gomez, A. Sanchez and D. Babot et al.,2003825359PigChamp, Inc., 20072007Ramos, A.M., R. Mestre, S. Gouveia, G. Evans and Y. Zhang et al.,200352255264Rothschild, M., C. Jacobson, D. Vaske, C. Tullge and L. Wang et al.,199693201205Rothschild, M.F., L. Messer, L. Day, R. Wales, T. Short, O. Southwood and G. Plastow,2000117577Saitou, N. and M. Nei,19874406425Sambrook, J. and D.W. Russell,20013rd Edn.,pp: 2100pp: 2100Short, T.H., M.F. Rothschild, O.I. Southwood, D.G. Mclaren and A. De-Vries et al.,19977531383142Sierra, A.C., T.B. Poot, Z.I. Diaz, A.H. Cordero and J.V. Delgado, 200554165170Swofford, D.L. and R.B. Selander, 198172281283Vogeli, P., H.U. Berschinger, M. Stamm, C. Stricker and C. Hagger et al.,1996Escherichia coli, causing edema disease and postweaning diarrhea in pigs, map to chomosome 6. ]]>27321328Yan, X.M., J. Ren, Y.M. Guo, N.S. Ding and K.F. Chen et al.,2003FUT1) gene in 26 pig breeds. ]]>30830834