Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.83.87Ganoderma
sp. MK05: Purification and Remazol Brilliant Blue R Decolorization]]>KhammuangSaranyu SarnthimaRakrudee 1200991The primary aim of this study was to screen for laccase
activity from various mushroom fruiting bodies found in Maha Sarakham
and nearby provinces of Northeast of Thailand. The enzyme activity was
followed by a spectroscopic property of an oxidized product of 2, 2-azino-bis
(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at 420 nm. The results
found that crude water extract of mushroom Ganoderma sp. MK05 gave
the highest specific activity of laccase. Consequence aim was to partial
purification of the crude water extract of the selected mushroom and to
evaluate the dye decolorization activity. The crude enzyme was partial
purified by ammonium sulfate fractionation in the range of 40-70% saturation
and followed with DEAE-cellulose anion exchange column to 1.34 and 3.07
purification folds, respectively. Native gel electrophoresis result showed
one protein band with laccase activity. The partial purified laccase from
this mushroom showed a high efficient decolorization ability of anthraquinone
dye, Remazol Brilliant Blue R with above 80% within 5 h at 30°C.]]>Bradford, M.M.,197672248254Camarero, S., D. Ibarra, A.T. Martinez, J. Romero, A. Gutierrez and J.C. DelRio,20074012641271Camarero, S., D. Ibarra, M.J. Martinez and A.T. Martinez,20057117751784Dodor, D.E., H.M. Hwang and S.I.N. Ekunwe,2004Trametes versicolor.]]>35210217Fragoeiro, S. and N. Magan,2008Trametes versicolor and Phanerochaete chrysosporium on differential breakdown of pesticide mixtures in soil microcosms at two water potentials and associated respiration and enzyme activity.]]>62376383Gutierrez, A., J. Rencoret, D. Ibarra, S. Molina and S. Camarero et al.,20074141244129Hong, F., Y. Zhang and Y.X. Zhu,2008Lentinula edodes.]]>136S696S697Khammuang, S. and R. Sarnthima,2007Lentinus polychrous Lev. and its potential for remazol brilliant blue R decolourisation.]]>6408413Mechichi, T., N. Mhiri and S. Sayadi,2006Trametes trogii.]]>649981005Nagai, M., M. Kawata, H. Watanabe, M. Ogawa and K. Saito et al.,2003Lentinula edodes fruit bodies.]]>14924552462Ncanana, S. and S. Burton,2007Trametes pubescens yields an antioxidant aromatic polymer.]]>446671Ng, T.B. and H.X. Wang,2004Cantharellus cibarius.]]>3133741Palmieri, G., G. Cennamo and G. Sannia,2005Pleurotus ostreatus and its oxidative enzymatic system.]]>361724Wang, H.X and T.B. Ng,2006Ganoderma lucidum.]]>72508513Wang, H.X. and T.B. Ng,2004Hericium erinaceum.]]>3221721Wang H.X. and T.B. Ng,2004Tricholoma giganteum.]]>315450454Wang, H.X. and T.B. Ng,2004Albatrella dispansus).]]>319381385Wang, H.X. and T.B. Ng,2006Pleurotus eryngii.]]>69521525Wu, Y., Y. Teng, Z. Li, X. Liao and Y. Luo,200840789796