Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.721.729AlkahtaniSaad 7200997The present study aims to evaluate the effect of antioxidation and hypomethylation on the genotoxicity and apoptosis induced in mice by arsenic trioxide (As2O3) in normal adult male SWR/J mouse. Animals were treated with intraperitoneally (ip) injected with (2.65, 5.35 or 10.70 mg kg-1 b.wt. of As2O3 which represent 0.25, 0.50 or 1 of LD50, respectively) and killed 24 h later. Another groups were treated with 30 mg kg-1 b.wt. of antioxidant and hypermethylizing agent butylated hydroxy toluene (BHT) 1 h prior to As2O3 administration. Another different groups were treated with three doses of 5-azacitidine (5-AzaC) 5 mg kg -1 b.wt. As2O3 administered after 6 days of the last dose. The three single doses of As2O3 significantly (p<0.05) increased the rate of total structural chromosomal aberrations (CAs) compared with the negative control. 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