Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.509.513KamelY.H. BazaraaH.M. ElwanA.E. FahmyN.A. ShakerO. 5200995In order to investigate the status and role of serum MMP-9 and urinary annexin V in steroid resistant nephrotic syndrome, 60 children aged between 2 and 15 years were enrolled in this study. Serum MMP-9 and urinary annexin V excretion were measured N patients were examined for apoptotic bodies by DNA in situ. Serum MMP-9 was significantly higher in patients than controls (189.1± 150.6 and 87± 53.7 pg mL-1, respectively, p<0.0001). Urinary annexin V was also significantly higher (4.5± 0.3 compared to 2.7± 0.6 ng g-1 creatinine, p<0.0001). Apoptosis by DNA in situ was positive in 29 children (48%) and positive cases tend to have higher MMP-9 levels (p = 0.05). Those with focal segmental glomerulosclerosis had the highest apoptosis rates (69%) and the lowest response to CPA (29%). Responders had higher urinary annexin V (p = 0.03) and biopsy evidence of apoptosis (p = 0.003) than non-responders. Negative apoptosis by DNA in situ predicted response with a likelihood ratio of 2.47. These data confirm the role of annexin V and MMP-9 in apoptosis in nephrotic syndrome. The role of MMP-9 in disease progression and that of TIMPs as adjunctive therapy need further elucidation.]]>Andreini, C., L. Banci, I. Bertini, C. Luchinat and A. Rosato,200432131Baker, A.J., A. Mooney and J. Hughes,19949421052116Bauvois, B., N. Mothu, J. Nguyen, T. Nguyen-Khoa, L.H. Noel and P. Jungers,20072211151122Bobkova, I.N., L.V. Kozlovskaia and O.A. Li,2008808696Borzecka, H., A. Bojarska-Junak, P. Sikora, M. Majewski, M. Zajaczkowska and J. Roliński,200459253256Chang, Y., B. Ceacareanu, D. Zhuang, C. Zhang, Q. Pu, A.C. Ceacareanu and A. Hassid,200626501507Daniel, C., J. Duffield, T. Brunner and T. Steinmann-Niggli,20012975768Ehrich, J.H., C. Geerlings, M. Zivicnjak, D. Franke, H. Geerlings and J. Gellermann,20072221832193Herzog, C., R. Seth, S.V. Shah and G.P. Kaushal,20077110091018Hodson, E.M. and J.C. Craig,20082313911394Kramer, S., Y. Wang-Rosenke, V. Scholl, E. Binder and T. Loof et al.,2008294F440F449Levick, S.P. and G.L. Brower,2008295H1375H1376Massova, I., L. Kotra and R. Fridman,19981210751095Matsuda, R., N. Kaneko, Y. Horikawa, F. Chiwaki, M. Shinozaki, S. Abe, W. Yumura, H. Nihei and T. Ieiri,20002982943Ohno, I., H. Ohtani and Y. Nitta,199716212219Ronco, P. and C. Chatziantoniou,200874873878Rost, S., C. Daniel, E. Schulze-Lohoff, H.G. Baumert, G. Lambrecht and C. Hugo,20026216591671Schedin, P., R. Strange, T. Mitrenga and P. Wolfe,2000113795806Schiffer, M., M. Bitzer and I.S. Roberts,2001108807816Simsek, B., M. Buyukcelik, M. Soran, A.K. Bayazit, A. Noyan, G. Seydaoglu and A. Anarat,2008237982Tawfeek, D.M. and S.M. Zaki,200512155165Thatte, U. and S. Dahanukar,199754511532Uzui, H., J. Lee, H. Shimizu and H. Tsutani,20001495159Vu, T.H. and Z. Werb,20001421232133Wasilewska, A.M. and W.M. Zoch-Zwierz,20082317951802Zachwieja, J., G. Dworacki, W. Bobkowski, A. Dobrowolska-Zachwieja, M. Zaniew and J. Maciejewski,200217197200Zachwieja, J., W. Bobkowski, M. Zaniew, A. Dobrowolska-Zachwieja, M. Lewandowska-Stachowiak and A. Siwińska,20031811161121