Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.504.508Sidaguri (Sida rhombifolia L.) as Antigout and Inhibition Kinetics of Flavonoids Crude Extract on the Activity of Xanthine Oxidase]]>IswantiniD. DarusmanL.K. HidayatR. 5200995Sida rhombifolia L., or Sidaguri in Indonesian, is a traditional medicinal plant with potential as a remedy against gout. The earlier research reported that flavonoids crude extract from this plant could in vitro inhibit the activity of Xanthine Oxidase (XO) (xanthine: oxygen oxidoreductase EC 1.2.3.2) up to 55% and could be antigout. It is a need to investigate type of inhibition kinetic of Sida rhombifolia L.s extract and the effects of fractionation on the inhibitory effect by in vitro method. The results showed that the flavonoids crude extract yielded approximately 12% with LC50 of 501 mg L-1 and its inhibitory effect from 48 to 71% (100-800 mg L-1). The kinetic study resulted that the type of flavonoids crude extract inhibition was a competitive inhibition with inhibitor affinity (α) of 2.32 and p<0.01. The fractionation yielded 11 fractions and the fraction 4 has highest activity, i.e., 79%. The fractions did not increase the inhibition effect significantly. GC-MS analysis of fraction 4 showed that there were 39 organic compounds and flavonoid fragments with retention times of 4.14, 6.53 and 6.74 that have resemble fragmentation of benzoic acid compound. By phytochemical assay, fraction 4 contain flavonoid.]]>Departemen Kesehatan Indonesia (Department of Health of Indonesia),19956th Edn.,Dharma, A.P.,1985Gonzaalez, A.G., I.L. Bazzocchi, L. Moujir, A.G. Ravelo, M.D. Correa and M.P. Gupta,1995462529Hanaee, J., M.R. Rashidi, A. Delazar and S. Piroozpanah,20044243247Harborne, J.B.,1987Hardman, J.G. and L.E. Limbird,19759th Edn.,Hayashi, T., K. Nagayama, M. Arisawa, M. Shimizu, S. Suzuki, M. Yoshizaki and N. Morita,198952210211Hsieh, J.F., S.H. Wu, Y.L. Yang, K.F. Choonga and S.T. Chen,20071534503456Iswantini, D. and L. Darusman,2003Sidaguri extract as an uric acid lowering agent on the activity of xanthine oxidase enzyme.]]>2003Iswantini, D., L. Darusman, M. Rahminiwati, Iskandar and R. Heryanto,2005Sidaguri (Sida rhombifolia L.) and celery (Apium Graveolens L.) as antigout: In vivo assay and bioactive compounds.]]>2005pp: 242253Lin, C.M., C.S. Chen, C.T. Chen, Y.C. Liang and J.K. Lin,2002294167172Lin, J.K., P.C. Chen, C.T. Ho and S.Y. Lin-Shiau,20004827362743Millar, T.M., J.M. Kanczler, T. Bodamyali, D.R. Blake and C.R. Stevens,200276570Noro, T., Y. Oda, T. Miyase, A. Ueno and S. Fukushima,19833139843987O'Driscoll, J.G., D.J. Green, J.M. Rankin and R.P. Taylor,199926779779Owen, P.L. and T. Johns,199964149160Pavia, D.L., G.M. Lampman and G.S. Kriz,19962nd Edn.,Silva, M.P., L. Mira, J. Lima and C.F. Manso,19961279284Soedibyo, M.,1998Tamta, H., S. Kalra and A.K. Mukhopadhyay,200571S49S54Theoduloz, C., P. Pacheco and G. Schmeda-Hirschmann,199133253255Finney, D.J.,19713rd Edn.,pp: 76-80pp: 76-80Connor, M.,200915646Aucamp, J., A. Gaspar, Y. Hara and Z. Apostolides,1997Camellia sinensis).]]>1743814385Markham, K.R.,1988