Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.319.325Glossaulax didyma Roding)]]>FujiiY. KawsarS.M.A. MatsumotoR. YasumitsuH. KojimaN. OzekiY. 4200994To find novel carbohydrate-binding proteins (lectins) from marine invertebrates to understand the binding mechanism of the protein and to apply it for glycan-dependent diagnostics and/or glycoconjugates capture technology. A D-galactoside-binding lectin was purified from foot of bladder moon shell, Glossaulax didyma by lactosyl-agarose affinity chromatography. The crude supernatant by Tris-buffered saline had strong hemagglutination activity against trypsinized and glutaraldehyde-fixed human erythrocyte. However, the activity was not inhibited by any tested saccharides and chilete reagents. On the other hand, the dialyzed crude supernatant obtained from the precipitates with 100 mM lactose in Tris-buffered saline had also hemagglutination activity inhibited by β-galactoside and D-galactose. The lectin was purified with lactosyl-agarose affinity chromatography. The molecular mass of the lectin was determined to be 60 kDa by SDS-PAGE under reducing and non-reducing conditions and being a 60 kDa polypeptide monomer by gel permeation chromatography. The association-rate constant (kass) and dissociation-rate constant (kdiss) determined for the lectin against asialofetuin was determined as 5.4x104 M-1sec-1 and 7.2x10-3sec-1, respectively. Lectin-conjugated Sepharose gel captured asialofetuin and eluted it by lactose-containing buffer from the gel, indicating that the lectin could catch the asialoglycoprotein. It was concluded that a many amount of a D-galactoside-binding lectin which can catch asialoglycoprotein presents in foot of the bladder moon shell.]]>Banerjee, S., S. Chaki, J. Bhowal and B.P. Chatterjee,2004Belamyia bengalensis: Purification and molecular characterization.]]>421125134Ebina, S., K. Matsubara, K. Nagayama, M. Yamaki and T. Gotoh,19959273677371Gilboa-Garber, N., D. Sudakevitz, C. Levene, N. Rahimi-Levene and V. Yahalom,2006Aplysia and Pseudomonas aeruginosa that detect I and P1 antigens.]]>221522Gilboa-Garber, N. and D. Sudakevitz,2001Aplysia lectin interaction with T antigen and poly-N-acetyllactosamine for screening of E. coli strains which bear glycoforms cross-reacting with cancer-associated antigens.]]>30235240Gourdine, J.P., G. Cioci, L. Miguet, C. Unverzagt and D.V. Silva et al.,20082833011230120Gourdine, J.P., A. Markiv and J. Smith-Ravin,200723831839Hwang, D.F., K.P. Tai, C.H. Chueh, L.C. Lin and S.S. Jeng,19912910191024Kawsar, S.M.A., Y. Fujii, R. Matsumoto, T. Ichikawa and H. Tateno et al.,2008Halichondria okadai.]]>150B349357Kim, J.Y., Y.M. Kim, S.K. Cho, K.S. Choi and M. Cho,2008Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni.]]>3211311141Kim, Y.M., K.I. Park, K.S. Choi, R.A. Alvarez, R.D. Cummings and M. Cho,2006Ruditapes philippirum is indicte upon infection with the protozoan parasite Perkinsus olseni.]]>2812685426864Laemmli, U.K.,1970227680685Matsui, T.,1984Urechis unicinctus.]]>166178188Melo, V.M., A.B. Duarte, A.F. Carvalho, A.F. Siebra and I.M. Vasconcelos,2000Aplysia dactylomela ring, 1828.]]>3814151427Moy, G.W., S.A. Springer, S.L. Adams, W.J. Swanson and V.D. Vacquier,200810519931998Naganuma, T., T. Ogawa, J. Hirabayashi, K. Kasai, H. Kamiya and K. Muramoto,2006Pteria peguin.]]>10607618Ozeki, Y., T. Matsui, M. Suzuki and K. Titani,1991Anthocidaris crassispina) eggs.]]>3023912394Ozeki, Y., T. Matsui, Y. Yamamot, M. Funahashi, J. Hamako and K. Titani,19955255261Ozeki, Y.,199741633640Sanchez, J.F., J. Lescar, V. Chazalet, A. Audfray and J. Gagnon et al.,2006Helix pomatia agglutinin. A hexameric lectin with a novel fold.]]>2812017120180Shinohara, Y., F. Kim, M. Shimizu, M. Goto, M. Tosu and Y. Hasegawa,1994223189194Smith, P.K., R.I. Krohn, G.T. Hermanson, A.K. Mallia and F.H. Gartner et al.,19851507685Takahashi, K.G., T. Kuroda and K. Muroga,2008150B4552Takeuchi, T., K. Hayama, J. Hirabayashi and K. Kasai,2008Caenorhabditis elegans N-glycans cotaining a Gal-Fuc disaccharide unit linked to the innermost GlcNAc residue are recognized by C. elegans galectin LEC-6.]]>18882890Wiechelman, K.J., R.D. Braun and J.D. Fitzpatrick,1988175231237Yamaura, K., K.G. Takahashi and T. Suzuki,2008Crassostrea gigas.]]>149B168175