Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2009.1.11in vitro Ovule Culture Technique for Producing Interspecific
Hybrid Between Tartary Buckwheat and Common Buckwheat]]>AsaduzzamanM. MinamiM. MatsushimaK. NemotoK. 1200991An interspecific hybrid of the Fagopyrum species
F. tataricum cv. CT-1 (2x and 4x) and F. esculentum cv.
Botansoba (2x) and GreatRuby (4x) was obtained through in vitro
ovule culture. Seven to ten days old ovules were excised and cultured
on 1/2 MS and MS media and rescued ovules were subjected to different
combinations and concentrations of BA, NAA, IAA and zeatin added individually
and in various combinations along with sucrose. Effects of the hormones
were used to evaluate their potentialities for direct regenerations, callus
induction and callus regeneration through ovule culture in interspecific
cross between tartary and common buckwheat. Interspecific hybridization
in Fagopyrum used ovule culture two methods development in this
study. The result showed that no single medium was adequate to ensure
complete development of the fertilized ovules to plantlets, thus necessitating
a sequential three step transfer to different media. RAPD-PCR analysis
confirmed hybridity of the regenerated plant.]]>Adachi, T., A. Yamaguchi, Y. Miike and F. Hoffman,1989Fagopyrum esculentum).]]>8247250Adachi, T., 19861986pp: 108116Baniya, B.K., K.W. Riley, D.M.S. Dongol and K.K. Sherchan,1992Fagopyrum ssp.) landraces.]]>1992pp: 6474Beasley, C.A. and I.P. Ting,1974in vitro fiber development from unfertilized cotton ovules. ]]>61188194Beasley, C.A. and I.P. Ting, 1973in vitro fiber development from fertilized cotton ovules.]]>60130139Beasley, C.A.,1971In vitro culture of fertilized cotton ovules.]]>21906907Campbell, C.,20032016Campbell, C., 1995Fagopyrum.]]>1995Chen, Q.F., 1998Fagopyrum (Polygonaceae) species native in China.]]>1998pp: 1831Chowdhury, C.N., A.K. Tyag, N. Maheshwari and S.C. Mheshwari,1993Oryza sativa L. cv. Pusa 169).]]>32357361Cheng, C. and R. Ni,19921992pp: 480483Choi, M.G., E.T. Koh and J.S. Ju,19881988Datta, S.K., K. Datta and I. Potrykus, 19909253256Egashira, H., R. Ogawa, H. Kanno, T. Tanisaka and S. Imanishi,1999Lycopersicon esculentum and Peruvianum-complex species (L. peruvianum and L. chilense).]]>118253258Fesenko, I.N., N.N. Fesenko and O. Ohnishi,2001Fagopyrum advances in buckwheat research.]]>2001pp: 404410Guan, L.M. and T. Adachi, 1992Fagopyrum esculentum) under summer conditions.]]>109304312Gundimeda, H.R., S. Prakash and K.R. Shivanna,1992Enarthrocarpus lyratus, a wild species and crop brassicas.]]>83655662Hirose, T., A. Ujihara, H. Kitabayashi and M. Minami,1995Fagopyrum.]]>456570Krauter, R., A. Steinmetz and W. Friedt,1991Helianthus via "embryo rescue" and characterization of the hybrids.]]>82521525Koh, E.T., M.K. Choi, J.S. Ju, C.S. Choi and Y.E. Park,19881988Kreft, I., 19831983pp: 312Lachman, S., 19911991pp: 145154Marshall, H.G. and Y. Pomeranz, 19825157210Marshall, H.G., 1969254126Murashige, T. and F. Skoog,196215473497Morris, M.R., 1951428589Nair, A., T. Ohmoto, S.H. Woo and T. Adachi, 1999162936Rumyantseva, N., N. Fedoseeva, G. Abdrakhmanova, V. Nikolskaya and S. Lopata, 1995Fagopyrum using in vitro embryo culture.]]>1995pp: 211220Ruszkowski, M., 19801980pp: 715Samimy, C., T. Bjorkman, D. Siritunga and L. Blanchard, 1996Fagopyrum esculentum with wild Fagopyrum tataricum.]]>91323330Samimy, C., 1991Fagopyrum esculentum with Fagopyrum tataricum: I. Site of pollen-tube arrest. II. Organogenesis from immature embryos of F. tataricum.]]>54215219Suvoeova, G.N., N.N. Fesenko and M.M. Kostrubin, 1994Fagopyrum esculentum Moench x Fagopyrum cymosum Meissn.).]]>141316Thengane, S., S.V. Paranjpe, S.S. Khuspe and A.F. Mascarenhas, 1986Gossypium species through in ovulo embryo culture.]]>6209219Ujihara, A., Y. Nakamura and M. Minami, 1990Fagopyrum properties of hybrids (F. esculentum Moench x F. cymosum Meissner) through ovule culture.]]>1990pp: 4551Wang, Y., R. Scarth and C. Campbell, 2004Fagopyrum esculentum and tetraploid F. homotropicum.]]>854148Wang, Y.J., R. Scarth and G.C. Campbell, 2002Fagopyrum tataricum (L.) Gaertn. and F. esculentum Moench.]]>193136Wang, Y.J. and C. Campbell,1998Fagopyrum esculentum, F. homotropicum and F. tataricum.]]>1998pp: 113Woo, S.H., Y.J. Wang and C.G. Campbell,1999Fagopyrum cymosum in the genus Fagopyrum.]]>161318