Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2008.846.856Dg3, a cDNA that Encodes a Novel Lipid Transfer Protein in Brassica napus]]>NamasivayamParameswari E. HankeDavid 5200885In this study, we have analysed the sequence of Dg3
clone using bioinformatic tools, determined copy number of this transcript
in the genome of B. napus and expression levels at various tissues/organ.
The cDNA contained 307-base pair open reading frame encoding 102 amino
acid residues, 60-base pair 5`-untranslated region and 127-base pair 3`-untranslated
region. The predicted mature protein has a molecular weight of 9.2 kDa
and is acidic, with a predicted isoelectric point (pI) of 6.2. The Dg3
sequencehas all the conserved structural characteristics of plant
LTPs and showed highest homology to LTPs from other plant species. The
transcripts of Dg3 were detected in all tested tissues but highest expression
was in siliques and in vitro embryogenic cultures. Possible roles
of Dg3 during somatic embryogenesis and normal plant development are discussed.]]>Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman,19972533893402Arondel, V., C. Vergenolle C. Cantrel and J.C. Kader,2000Arabidopsis thaliana.]]>157112Bernhard, W.R., S. Thoma, J. Botella and C.R. Somerville,199195164170Bishop-Hurley, S.L., R.C. Gardner and C. Walter,2003Pinus radiata.]]>74267281Blanckaert, A., L. Belingheri, P.E. Sautiere, J. Vasseur and J.L. Hilbert,200240339345Canevascini, S., D. Caderas, T. Mandel, A.J. Fleming, I. Dupuis and C. Kuhlemeier,1996112513524Coutos-Thevenot, P., T. Jouenne, O. Maes, F. Guerbette, M. Grosbois, J.P. Le Caer, M. Boulay, A. Deloire, J.C. Kader and J. Guern,1993217885889Chomczynski, P. and N. Sacchi,1987162156159Chugh, A. and P. Khurana,200283715730Dawes, C.J. and E. Bowler,1959Raphanus sativus.]]>46561565Dodeman, V.L., G. Ducreux and M. Kreis,1997versus somatic embryogenesis.]]>4814931509Dong, J.Z. and D.I. 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