Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2008.563.569Glomus intraradices]]>Gomez-LeyvaJ.F. Lara-ReynaJ. Hernandez-CuevasL.V. Martinez-SorianoJ.P. 3200883Specific primers and Polymerase Chain Reaction (PCR)
assays that identify Arbuscular Mycorrhizal (AM) fungi Glomus intraradices
were developed. Monoxenic cultures of fungi G. intraradices and
Gigaspora gigantea in association with Ri T-DNA transformed carrot
roots were established in order to obtain fungal DNA free of host and
others contaminants. RAPD analysis using 10 AM fungi from genera Glomus,
Gigaspora and Acaulospora allowed the determination of two
amplified fragments that were specific to G. intraradices. The
DNA fragments were cloned, sequenced and subsequently used to design SCAR
species-specific primers. A set of primers, GIN630F and GIN630R,
drove the amplification of a 630 bp fragment specific for G. intraradices,
which was absent when DNA of other AM fungi or plants were used as templates.
The assay allowed the detection of G. intraradices in colonized
roots of carrot. The SCAR-based protocol described here may be a tool
of great value in studies of Glomeromycota`s molecular systematic and
ecology.]]>Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman,19972533893402Antoniolli, Z.I., D.P. Schachtman, K. Ophel-Keller and S.E. Smith,2000Glomus mosseae and Gigaspora margarita spores from a permanent pasture.]]>104708715Bago, B., C. Azcon-Aguilar, A. Goulet and Y. Piche,1998139375388Bécard, G. and Y. Piché,19921992pp: 89-91pp: 89-91Becard, G., P. Douds and P. Pfeffer,1992in vitro hyphal growth of vesicular-arbuscular mycorrhizal fungi in the presence of CO2 and flavonols.]]>58821825Berbee, M.L. and J.W. Taylor,199573S677S683Chabot, S., R. Bel-Rhlid, R. Chenevert and Y. Piché,1992in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker and Hall, by activity of structurally specific flavonoid compounds under CO2-enriched conditions.]]>122461467Doyle, J.J.,1990121315Gandeboeuf, D., C. Dupre, P.N. Roeckel-Drevet and G. Chevalier,199743723728Gehrig, H., A. Schübler and M. Kluge,1996Geosiphon pyriforme, a fungus forming endocytobiosis with Nostoc (cyanobacteria), is an ancestral member of the Glomales: Evidence by SSU rRNA analysis.]]>437181Gerdemann, J.W. and T.H. Nicolson,1963Endogone species extracted from soil by wet sieving and decanting.]]>46235244Hamill, D.J., S. Rounsley, A. Spencer, G. Todd and M.J. Rhodes,199110221224Jansa, J., A. Mozafar, T. Anken, R. Ruh, I. Sanders and E. Frossard,200212225234Juge, C., J. Samson, C. Bastien, H. Vierheilig, A. Coughlan and Y. Piché,2002Glomus intraradices: A critical cold-storage period.]]>123742Lee, S. and J. Taylor,19911991pp: 282-287pp: 282-287Lloyd-MacGilp, S.A., S.M. Chambers, J.C. Dodd, A.H. Fitter, C. Walker and J.P.W. Young,1996Glomus mosseae related mycorrhizal fungi.]]>133103111Lovelock, C.E. and J.J. Ewel,2005167219228Murashige, T. and F. Skoog,196215473497Nazar, R.N., X. Hu, J. Schmidt, D. Culham and J. Robb,1991Verticillium wilt pathogens.]]>39111Nin, S., A. Bennici, G. Roselli, D. Mariotti, S. Schiff and R. Magherini,1997Agrobacterium-mediated transformation of Artemisia absinthium L. (wormwood) and production of secondary metabolites.]]>16725730Paran, I. and R.W. Michelmore,199385985993Poulin, M., R. Bel-Rhlid, Y. Piche and R. Chenevert,1993Daucus carota seedlings stimulate hyphal development of vesicular arbuscular mycorrhizal fungi in the presence of the optimal CO2 enrichment.]]>1923172327Reddy, S.R., P.K. Pindi and S.M. Reddy,20058916991709Sanders, I.R.,20038143145Schüβler, A., D. Schwarzott and C. Walker,2001Glomeromycota: Phylogeny and evolution.]]>10514131421Schuβler, A., H. Gehrig, D. Schwarzott and C. Walker,2001105515Sieverding, E. and F. Oehl,2006Entrophospora and description of Kuklospora and Intraspora, two new genera in the arbuscular mycorrhizal Glomeromycetes.]]>806981Simon, L., M. Lalonde and T. Bruns,199258291295Simon, L., R.C. Levesque and M. Lalonde,19935942114215Simon, L.,199613395101St-Arnaud, M., C. Hamel, B. Vimard, M. Caron and J.A. Fortin,1996Glomus intraradices in an in vitro system in the absence of the host roots.]]>100328332Wang, B. and Y.L. Qiu,200616299363White, T.J., T.D. Bruns, S.B. Lee and J.W. Taylor,19901990pp: 315-322pp: 315-322