Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2008.556.562BararJaleh OmidiYadollah 3200883In the current investigation, primary porcine brain
capillary endothelial (PBCE) cells were isolated and used as an in
vitro blood-brain barrier model. The bioelectrical and permeability
properties were assessed in PBCE cells treated with Astrocytes Conditioned
Medium (ACM), hydrocortisone and cAMP elevators. The primary PBCE cells
cultured with ACM plus cAMP elevators provided significantly higher trans-endothelial
electrical resistance (~700Ω cm2) in comparison with
those cells cultured with ACM (~400 Ω cm2) or untreated
control (~200 Ω cm2), while PBCE cells cultured with ACM
plus hydrocortisone (550 nM) resulted in ~550 Ω cm2. The
bioelectrical properties were further validated by means of the permeability
coefficients of transcellular and paracellular markers with results of
~20 and ~5 (x10-6 cm sec-1) for propranolol and
sucrose, respectively in PBCE cells treated with ACM and cAMP elevators.
Upon the obtained bioelectrical and permeability results, PBCE cells cultured
with astrocytes conditioned medium and cAMP elevators can be proposed
as a high-throughput in vitro model for drug screening and brain
targeting.]]>Allt, G. and J.G. Lawrenson,200052112Audus, K.L. and R.T. Borchardt,1987507918De Boer, A.G. and P.J. Gaillard,200747323355Franke, H., H.J. Galla and C.T. Beuckmann,1999in vitro-model of the blood-brain barrier: Transport studies on retinoids, sucrose, haloperidol, caffeine and mannitol.]]>8186571Franke, H., H. Galla and C.T. Beuckmann,2000in vitro.]]>5248256Gumbleton, M. and K.L. Audus,2001in vitro cell cultures to serve as a permeability screen for the blood‐brain barrier.]]>9016811698Hamm, S., B. Dehouck, J. Kraus, K. Wolburg-Buchholz, H. Wolburg, W. Risau, R. Cecchelli, B. Engelhardt and M.P. Dehouck,2004315157166Hoheisel, D., T. Nitz, H. Franke, J. Wegener, A. Hakvoort, T. Tilling and H.J. Galla,1998244312316Hurst, R.D. and I.B. Fritz,19961678188Ishizaki, T., H. Chiba, T. Kojima, M. Fujibe, T. Soma, H. Miyajima, K. Nagasawa, I. Wada and N. Sawada,2003290275288Jeliazkova-Mecheva, V.V. and D.J. Bobilya,2003129198Kiessling, F., J. Kartenbeck and C. Haller,1999297131140Newman, G.R., L. Campbell, C. von Ruhland, B. Jasani and M. Gumbleton,1999295111120Omidi, Y., L. Campbell, J. Barar, D. Connell, S. Akhtar and M. Gumbleton,2003in vitro blood-brain barrier model for drug uptake and transport studies.]]>99095112Omidi, Y. and M. Gumbleton,20052005Rubin, L.L., D.E. Hall, S. Porter, K. Barbu and C. Cannon et al.,199111517251735Siakotos, A.N.,197432717722Smith, M., Y. Omidi and M. Gumbleton,200715253268Suda, K., B. Rothen-Rutishauser, M. Gunthert and H. Wunderli-Allenspach,200137505514Takagi, M., H. Sugihara and K. Tabuchi,199131553558Takakura, Y., K.L. Audus and R.T. Borchardt,199122137165Tilling, T., D. Korte, D. Hoheisel and H.J. Galla,1998in vitro.]]>7111511157Tsuji, A., T. Terasaki, Y. Takabatake, Y. Tenda and I. Tamai et al.,19925114271437Weidenfeller, C., S. Schrot, A. Zozulya and H.J. Galla,20051053162174Wolburg, H., J. Neuhaus, U. Kniesel, B. Krauss and E.M. Schmid et al.,199410713471357