Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2008.1211.1215Saccharomyces cerevisiae]]>EllertonS.S. LitewkaA.J. GonzalezM. JueC.K. 7200887The GDP-mannose transporter gene VRG4 has been
identified from a number of yeasts including Saccharomyces cerevisiae.
The vrg4-2 alleleof the GDP-mannose transporter of S.
cerevisiae has been primarily characterized as having general phenotypes
affecting glycosylation. In this study, vrg4-2 was characterized,
specifically with regard to defects in cell wall biosynthesis. The cell
wall in vrg4-2 mutants was found to be profoundly compromised,
as measured by the spheroplast lysis assay. These mutant cells exhibited
increased sensitivity to Congo red which is believed to interfere with
chitin-glucan assembly in the cell wall. An increased level of cell wall
fluorescence was also observed when the cells were incubated with Calcofluor
white suggesting an increased level of chitin in the wall. Despite these
apparent major alterations in the cell wall, the vrg4-2 mutants
exhibited near normal growth relative to the wild-type. All together,
these results suggest that vrg4-2 mutation affects the cell wall
structure, but less so in general cellular metabolism.]]>Adams, A., D. Gottschling, C. Kaiser and T. Stearn,19971st Edn.,Arakawa, K., M. Abe, Y. Noda, H. Adachi and K. Yoda,200652137145Baldwin, T.C., M.G. Handford, M.I. Yuseff, A. Orellana and P. Dupree,20011322832295Cottrell, T.R., C.L. Griffith, H. Liu, A.A. Nenninger and T.L. Doering,20076776785Dean, N., Y.B. Zhang and J.B. Poster,19972723190831914Duran, A. and C. Nombela,200415030993103Francois, J.M.,2006129953000Gemmill, T.R. and R.B. Trimble,19991426227237Gonzalez, M. and P.N. Lipke,2003Saccharomyces cerevisiae.]]>2003pp: 101101Herrero, A.B., D. Uccelletti, C.B. Hirschberg, A. Dominguez and C. Abeijon,20021420431Imai, K., Y. Noda, H. Adachi and K. Yoda,200528082758284Jackson-Hayes, L., T.W. Hill, D.M. Loprete, L.M. Fay and B.S. Gordon, et al,200815420372047Klis, F.M., P. Mol, K. Hellingwerf and S. Brul,200226239256Lim, S.T., C.K. Jue, C.W. Moore and P.N. Lipke,199517735343539Nishikawa, A., B. Mendez, Y. Jigami and N. Dean,200219691698Nishikawa, A., J.B. Poster, Y. Jigami and N. Dean,20021842942Popolo, L., D. Gilardelli, P. Bonfante and M. Vai,1997179463469Poster, J.B. and N. Dean,199627138373845Pringle, J.R.,1991194732735Ram, A.F. and F.M. Klis,2006122532256Terrance, K., P. Heller, Y.S. Wu and P.N. Lipke,1987169475482Terrance, K. and P.N. Lipke,198716948114815Walker, L.A., C.A. Munro, I. de Bruijn, M.D. Lenardon, A. McKinnon and N.A. Gow,2008Candida albicans from echinocandins.]]>2008Zlotnik, H., M.P. Fernandez, B. Bowers and E. Cabib,198415910181026