Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2008.1193.1198Mucor racemosus PTCC 5305]]>SetayeshN. SepehrizadehZ. YazdiM. Tabatabaei 7200887The present research aims to study the molecular characterization
of a new 60S ribosomal protein L10 (RPL10) from Mucor racemosus
PTCC5305 and its coding sequence. A full-length complementary DNA (cDNA)
encoding 60S ribosomal protein L10 was isolated from Mucor racemosus
cDNA library. The cDNA of 766 base pairs contains a 652 base pairs
open reading frame and 5`- and 3`- flanking regions including a polyadenylation
sequence. The deduced protein of 216 amino acid residues had an isoelectric
point (pI) of 10.16 and a calculated molecular mass of about 24.5 kDa.
Sequence analysis of RPL10 revealed 78% identity and 89% similarity with
fungus Pyrenophora tritici. Also it showed 73% identity and 84%
similarity with Homo sapiens. Phylogenetic tree analysis based
on the conserved domain of Ribosomal_L16_L10e super family exhibited that
Mucor racemosus RPL10 is closely related to that of Candida
glabrata.]]>Casale, W.L., D.G. Mcconnell, S.Y. Wang, Y.J. Lee and J.E. Linz,1990Mucor racemosus which exhibits striking similarity to human ras genes.]]>1066546663Cujec, T.P. and B.M. Tyler,1996Neurospora crassa.]]>24943950Chávez-Rios, R., L.E. Arias-Romero, M.J. Almaraz-Barrera, R. Hernández-Rivas and N. Guillén et al.,2003Entamoeba histolytica share structural and functional homologies with QM/Jif-1: Proteins with extraribosomal functions.]]>127151160Cihlar, R.L.,1985Mucor.]]>1985pp: 449-467pp: 449-467Dowdy, S.F., K.M. Lai, B.E. Weissman, Y. Matsui and B.L. Hogan et al.,19911957635769Eisinger, D.P., F.A. Dick and B.L. Trumpower,19971751365145Fromont-Racine, M., B. Senger, C. Saveanu and F. Fasiolo,20033131742Geourjon, C. and G. Deleage,199511681684Harms, J., F. Schluenzen, R. Zarivach, A. Bashan and H. Bartels et al.,2002525176178Hofer, A., C. Bussiere and A.W. Johnson,20072823263032639Inada, H., J. Mukai, S. Matsushima and T. Tanaka,1997230331334Johnson, A.W., E. Lund and J. Dahlberg,200227580585Karsi, A., A. Patterson, J. Feng and Z. Liu,2002291177186Lecompte, O., R. Ripp, J.C. Thierry, D. Moras and O. Poch,20023053825390Lillico, S.G, J.C. Mottram, N.B. Murphy and S.C.Welburn,2002Trypanosoma brucei.]]>211123128Maguire, B.A. and R.A. Zimmermann,2001104813816Manchado, M., C. Infante, E. Asensio, J.P. Cañavate and S.E. Douglas,2007Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.) (Teleostei: Pleuronectiformes): Phylogeny and tissue-and development-specific expression.]]>7107107Mao, H. and J.R. Williamson,1999292345359Marchler-Bauer, A., J.B. Anderson, P.F. Cherukuri, C. DeWeese-Scott and L.Y. Geer et al.,200533D192D196Milne, A.N., W.W. Mak and J.T. Wong,19751228992Monteclaro, F.S. and P.K. Vogt,19939067266730Nguyen, Y.H., A.A. Millsand and E.J. Stanbridge,199868281285Nishimura, M., T. Kaminishi, C. Takemoto, M. Kawazoe and T. Yoshida et al.,2008377421430Oh, H.S., H. Kwon, S.K. Sun and C.H. Yang,20022773648936498Olsen, G.J. and C.R. Woese,19937113123Patterson, A., A. Karsi, J. Feng and Z. Liu,2003305151160Sambrook, J., E.F. Fritsch and T. Maniatis,20013rd Edn.,Semrad, K., R. Green and R. Schroeder,2004Escherichia coli.]]>1018551860Wool, I.G., Y.L. Chan and A. Gluck,199573933947Zarivach, R., A. Bashan, R. Berisio, J. Harms and T. Auerbach et al.,200417901912Zemp, I. and U. Kutay,200758127832793