Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2008.1149.1157A. Al-OmairMohammed M. YoussefMagdy 7200887The present study was designed to investigate the effect
of selenium (Se) administration (as sodium selenite: 0.5 or 1 mg kg-1
body weight) or/and α-difluromethylornithine (DFMO: 2 mg kg-1
body weight) on the elevated polyamine levels. For this purpose the polyamine
(putrescine, spermidine, spermine) levels were elevated in female Swiss
albino mice by adding 2% of both L-arginine and L-ornithine to the drinking
water for four weeks. The elevated putrescine and spermidine levels were
decreased significantly by administration low and high dose of Se. The
DFMO abolished the concentrations of both putrescine and spermidine in
liver and kidney of the experimental mice. The combination of Se and DFMO
normalized the polyamine levels. On the other hand, the spermine level
was increased in liver and kidney by administration of Se or/and DFMO.
To elucidate the effects of the elevated polyamines on the oxidative enzymes,
the activity of glutathione peroxidase (GSH-PX), glutathione reductase
(GSH-R) and superoxide dismutase (SOD) in the liver and the kidneys of
the experimental mice were determined. The GSH-Px, GSH-R and SOD activities
were increased by Se administration compared to the control. Conversely,
DFMO produced an inhibition in the activity of antioxidative enzymes.
These results suggest that the combination between Se and DFMO appear
to be additive chemopreventive effect to reduce the elevated polyamine
levels. This combination will protect the tissues from the deleterious
effect of high polyamine levels and improve the activities of the cellular
antioxidative system.]]>Ackermann, J.M., A.E. Pegg and D.E. McCloskey,20035461468Bagni, N. and A. Tassoni,200120301317Battell, M.L., H.L. Delgataty and J.H. McNeill,19981792734Belle, N.A., G.D. Dalmolin, G. Fonini, M.A. Rubin and J.B. Rocha,20041008245251Bergman, L.B., U.B. Torndal, S. Eken, C. Nyström and A. Capitanio et al.,200526125131Canizares, F., J. Salinas, H.M. de las, J. Diaz and I. Tovar et al.,1999520352041Casero, R.A. and L.J. Marton,20076373390Chattopadhyay, M.K., C.W. Tabor and H. Tabor,2006Saccharomyces cerevisiae.]]>23751761D'Agostino, L., M. Di Pietro and A. Di Luccia,200527237773787Davidson, N.E., H.A. Hahm, D.E. McCloskey, P.M. Woster and R.A. Casero Jr.,199966973Gerner, E.W. and L.F. Meyskens,20044781792Fleury, C., B. Mignotte and J.L. Vayssiere,200284131141Ganther, H.E.,19992016571666Glikman, P., I. Vegh, M.A. Pollina, A.H. Mosto and C.M. Levy,19876022372243Grimble, R.F. and G. Grimble,19984605610Ha, H.C., N.S. Sirisoma, P. Kuppusamy, J.L., Zweier and P.M. Woster et al.,1998951114011145Halline, A.G., P.K. Dudeja and T.A. Brasitus,1989259513518Heby, O.,198119120Ivanov, S., G.I. Botchkina, Y. Al-Abed, M. Meistrell and F. Batliwalla et al.,1998188327340Jacoby, R.F., D.J. Marshall, M.A. Newton, K. Novakovic and K. Tutsch et al.,199656710714Jun, J. Y., J.W. Griffith, R. Bruggeman, S.L. Washington and M. Demers et al.,2007in vitro and in vivo biological properties of 4T1 murine mammary cancer cells.]]>1052936Jung, I.L. and I.G. Kim,2003ahpC, katG and katE genes in Escherichia coli is regulated by polyamines: Polyamine-deficient mutant sensitive to H2O2-induced oxidative damage.]]>301915922Kajander, S.O., R. Harvima, L. Kauppinen, K.K. Akerman and H. Martikainen et al.,1990267767774Kakkar, P., B. Das and P.N. Viswanathan,198421130132Karni, L., S.J. Moss and E. Tel-Or,1984Nostoc muscorum.]]>140215217Kashiwagi, K., S. Miyamoto, E. Nukui, H. Kobayashi and K. Igarashi,1993Escherichia coli.]]>2681935819363Kirchner, D.L., M.D. Mercieca, J.A. Crowell and B.S. Levine,199950127135Krause, T., K. Lüersen, C. Wrenger, T.W. Gilberger and S. Müller et al.,2000352287292Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Mamont, P.S., C. Danzin, J. Wagner, M. Siat, A.M. Joder-Ohlenbusch and N. Claverie,1982S-adenosyl-L-methionine in mammalian cells as a consequence of the inhibition of putrescine biosynthesis.]]>123499504Manni, A., S. Washington, J.W. Griffith, M.F. Verderame and D. Mauger et al.,2002in vitro and in vivo features of aggressive and metastatic behavior by human breast cancer cells.]]>1995105Manni, A., S. Washington, X. Hu, J.W. Griffith and R. Bruggeman et al.,200522255263Manni, A., T. Rager, S. Kimball, L. Jefferson and S. Washington et al.,20073111871191McCann, P.P. and A.E. Pegg,199254195215Metcalf, B., P. Bey, C. Danzin, M. Jung and P. Casara et al.,197810025512553Meyskens, F.L. and E.W. Gerner,19995945951Moinard, C., L. Cynober and J. De Bradt,200524184197Muller, I.B., R.D. Gupta, K. Luersen, C. Wrenger and R.D. Walter et al.,2008Plasmodium falciparum as chemotherapeutic target.]]>16017Nikolic, J., I. Stojanovic, R. Pavlovic, D. Sokolovic and G. Bjelakovic et al.,200732127131Oredsson, S.M., M. Kanje, P.S. Mamont, J. Wagner and O. Heby et al.,1986708996Pegg, A.E., K.A McGovern and L. Wiest,1987241305307Pegg, A.E.,198848759774Ramya, T.N.C., N. Surolia and A. Surolia,2006Plasmodium falciparum.]]>348579584 Redman, C., J.A. Scott, A.T. Baines J.L. Basye and L.C. Clark I>et al.,1998125103110Rotruck, J.T., A.L. Pope, H.E. Ganther, A.B. Swanson, D.G. Hafeman and W.G. Hoekstra,1973179588590Satriano, J., S. Matsufuji, Y. Murakami, M.J. Lortie and D. Schwartz et al.,19982731531315316Schertel, B. and W. Eichler,19913722733Seiler, N., S. Sarhan, C. Grauffel, R. Jones and B. Knodgen et al.,19905050775083Singh, J., G. Kelloff and B. Reddy,1993ras oncogene by chemopreventive agents during azoxymethane induced colon carcinogenesis.]]>14699704Sokolovic, D., G. Bjelakovic, J. Nikolic, B. Djindjic and D. Pavlovic et al.,20082008Tabor, C.W. and H. Tabor,199968132Teixeira, D., M.L. Santaolaria, V. Meneu and E. Alonso,200213237153720Thomas, T. and T.J. Thomas,200158244258Tkachenko, A., L. Nesterova and M. Pshenichnov,2001Escherichia coli.]]>176155157Wallace, H.M., A.V. Fraser and A. Hughes,2003376114Wu, G. and S.M. Morris Jr.,1998336117Yerlikaya, A. and B.A. Stanley,200613313317Zeng, H.,2002132674679