Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2007.653.657In vitro Clonal Propagation of Cucumis sativus L. By Shoot Tip Culture]]>MohammadiJafar SivritepeNuray 4200774The possibility of in vitro clonal propagation of Cucumis sativus L. cultivars Telegraph 314, Pepinex 69 and Rebella by the use of shoot tip explants was investigated as an interesting strategy for reducing the cost of hybrid seed production. Shoot tip explants (3 to 5 mm length) from 13-day-old in vitro grown seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different BA and NAA concentrations (0.0, 0.3, 0.4 and 0.6 μM) and their combinations for 32 days. To investigate the effect of BA (6-Benziladenine) and NAA (•-Naphthaleneacetic acid) on shoot proliferation the medium supplemented with 0.0, 0.3, 0.4 and 0.6 μM of each Plant Growth Regulator (PGR) alone and in combination. The proliferation rate, shoot quality and other parameters studied indicate that the optimal treatment was 0.4 μM BA. The proliferated shoots succeeded to root in each treatment with different frequencies (45-100%) although the highest rooting frequency was obtained from the PGR-free medium. The plantlets were gradually acclimated ex vitro and successfully established under greenhouse conditions.]]>Ahmad, A. and M. Anis,2005In vitro mass propagation of Cucumis sativus L. from nodal segments.]]>29237240Anonymous,20052005Bhatia, P., N. Ashwanth, T. Senaratna and D. Midmore,2004Lycopersicon esculentum).]]>78121Burza, W. and S. Malepszy,1995Cucumis sativus L.) is free of stable genetic-variation.]]>114341345Burza, W. and S. Malepszy,1995In vitro culture of Cucumis sativus L. plants from protoplasts through direct somatic embryogenesis.]]>41259266Chu, Y.F., J. Sun, X.Z. Wu and R.H. Liu,20025069106916Debeaujon, I. and M. Branchard,19933491100Filipecki, M., A. Wisniewska, Z.M. Yin and S. Malepszy,2005in vitro culture.]]>82349356Handley, L.W. and O.L. Chambliss,1979In vitro propagation of Cucumis sativus L.]]>142223Konstas, J. and S. Kintzios,2003Cucumis sativus L.): The effect of growth retardants, liquid culture and vessel size.]]>21538548Kumar, H.G.A., H.N. Murthy and K.Y. Paek,2003Cucumis sativus L.]]>98213222Ladyzynski, M., W. Burza and S. Malepszy,2002125349356Milner, J.A.,2000711654516595Mohiuddin, A.K.M., M.K.U. Chowdhury, Z.C. Abdullah and S. Napis,1997in vitro shoot regeneration.]]>517578Murashige, T. and F. Skoog,196215473497Plader, W., S. Malepszy, W. Burza and Z. Rusinowski,1998Cucumis sativus L.).]]>103915Punja, Z.K., N. Abbas, G.G. Sarmento and F.A. Tang,1990Cucumis sativus, vars, sativus and hardwickii, C. melo and C. metuliferous from explants through somatic embryogenesis and organogenesis. Influence of explant source, growth regulator regime and genotype.]]>2193102Sarowar, S., H.Y. Oh, N.I. Hyung, B.W. Min, C.H. Harn, S.K. Yang, S.H. Ok and J.S. Shin,2003In vitro micropropagation of a Cucurbita interspecific hybrid cultivar: A root stock plant.]]>75179182Selveraj, N., A. Vasudevan, M. Manickavasagam and A. Ganapathi,2006In vitro organogenesis and plant formation in cucumber.]]>50123126Vasudevan, A., N. Selvaraj, A. Ganapathi, S. Kasthurirengan, V.R. Anbazhagan and M. Manickavasagam,2004Cucumis sativus L.]]>48125128Zhu, C. and Z. Chen,2005in vitro.]]>814553