Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2007.451.453Jalal HosseinimehrSeyed BagheriGholamali GholipoorMohammad MokaramiHassan 2200772A simple and sensitive spectrophotometric method has been used for the determination of iron concentration in wheat flour and compare with atomic absorption method. The method is based on the reaction of iron with o-phenathroline reagent at acidic condition. The absorbance-concentration plot is linear over the range 0.3-2 mg mL-1 with correlation coefficient of 0.995. This method was applied successfully for determination of iron in flour samples. Quantification of the same flour was determined by atomic absorption for iron content. Values of iron content measured in the flour samples with spectrophotometer method are in good agreement with results obtained with atomic absorption method. It was not statistically significant difference between visible spectrophotometric and atomic absorption methods. This method can be successfully applied determination of iron in flour samples and can use instead atomic absorption.]]>Beinner, M.A. and J.A. Lamounier,200324268274Cook, J.D. and M.E. Reusser,198338648659Davidson, F. and R. Miller,20052005McPhail, A.P. and T.H. Bothwell,19921992pp: 1-12pp: 1-12Rao, A.N.,2005419Riganakos, K.A. and P.G. Veltsistas,200382637643Strong, F.C. and N.J. Martin,199037711718Tesfaldet, Z.O. and J.F. Staden,20046411891195Yip, R.,199751S16S24