Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2006.347.350Bacillus Phages in Different Temperatures and pH-values]]>AkelHazem HunaityAfnan AbdullahInas DokerNaimat 2200662Thirteen thermotolerant Bacillus phages were
isolated from Jordanian hot springs. These phages were tested for their
resistance to the presence of high concentrations of sodium azide at different
temperatures and pH-values. At 43 °C and pH 6.5, almost all phages
were still active in the presence of 1% sodium azide but inactive moderately
or immediately when treated with 3 or 5% sodium azide, respectively. The
exception was for the phage 18 that showed resistance to 3% sodium azide
and slight growth in the presence of 5% sodium azide. The phage 18, treated
with 3% sodium azide, was able to resist the high temperatures 103 and
113 °C and showed moderate growth when assayed at 43 °C. Different
observations were also seen at different pH-values for Bacillus
phages treated with 1% sodium azide, phage 16 was unable to grow in alkaline
condition (pH = 11) on the other hand, only three phages (18, 20 and 30)
were able to grow in acidic condition (pH = 2). For the phage 18 treated
with 3% sodium azide was able to grow at pH values 6, 8 and 9, respectively.
These results may reflect their high resistance of their protein components.]]>Archbold, J., R. Lewis, T. McManus and B. Pfeiffer,2002317Dunn, I.S.,19967547553Edwards, S.J.,1938Streptococcus mastitis by cultural methods.]]>51250263Jian, S.C. and J.H. Paul,19986427802787Hazem, A.,2002Bacillus bacteriophages.]]>25469476Kuttler, E.,19971997Mallmann, W.L.,194012875878Price, C. and J. Rooyen,20012001Rapora, M.P. and E.W. Robert,199317518561859Snyder, M.L. and H.C. Lichstein,194067113115