Journal of Biological Sciences1727-30481812-5719Asian Network for Scientific Information10.3923/jbs.2004.676.680In vitro Plantlet Regeneration from Internode Explant of Native-olive
(Elaeocarpus robustus roxb.)]]>M. RahmanM. N. AminM. AhmedR. A.K. AzadM. BegumF. 5200445Regeneration of multiple shoots via callus induction and organogenesis were achieved in native-olive (Elaeocarpus robustus). Callus induction and shoot buds regeneration were obtained from internode explants of Elaeocarpus robustus on their sufficient medium. The best organic callus was found on modified MS (MMS1) medium supplemented with 0.5 mg L1 BA+0.5 mg L1 2,4-D. Development of adventitious shoots occurred when the calli were subcultured on MMS1 medium supplemented with BA and NAA. Maximum frequency (80%) of calli induced adventitious shoots with highest number of 14.05 ± 1.56 shoots per callus were obtained when the medium was fortified with 1.0 mg L1 BA+0.1 mg L1 NAA. Plantlets developed roots when in vitro developed microcuttings were implanted on modified MS (MMS2) medium with 0.2 mg L1 of IBA. Within six weeks of transfer, 65% rooting was achieved on this medium. Rooted shoots (plantlets) were gradually acclimatized and successfully established under natural condition with about 50% survival rate.]]>Litz, R.E., G.A. Moore and C. Srinivasan,1985In vitro systems for propagation and improvement of tropical fruits and palms.]]> 7157198Rao, B.G. and V.L. Chopra,198717710Pandey, R. and P.S. Gonopathy,1984Cicer arietimum L. CV. BG-203.]]>34547Barlass, M. and K.G.M. Skene,1982In vitro plantlet formation from Citrus species and hybrids.]]>17333341Raj Bhansali, R. and H.C. Arya,1979Citrus limettioides Tanaka (sweet lime) callus culture.]]>2897100Azad, M.A.K. and M.N. Amin,1998In vitro regeneration of plants from internode explants of Adhatoda vasica Nees.]]>82734Litz, R.E. and R.A. Conover,1980Averrhoa carambols.]]>15735735Islam, R., M.R. Karim, M. Hossain and O.I. Joarder,1992Aegle marmelos Corr.]]>25559Kouider, M., S.S. Korban, R.M. Skirvin and H. Joung,1985in vitro.]]>1109396Amin, M.N. and V.S. Jaiswal,1987in vitro shoot proliferation on nodal explants of mature trees.]]>9235243Norton, E.M. and C.R. Norton,1986in vitro subculture of shoots of woody species of Rosaceae.]]>5187197Amin, M.N., M.A. Habib, M.A.K. Azad and M. Akhteruzzaman,2001Ficus religiosa L. Through tissue culture.]]>2001pp: 99Khatun, A. and M.T. Hossain,2001In vitro micropropagation of pomegranate (Punica granatum).]]>2001pp: 2727Hutchinson, J.F.,19811981pp: 113120Khan, M.S. and M.K. Alam,19961996p: 57p: 57Ghani, A., 1998pp: 167-168pp: 167-168Rowe-Dutton, P.,19761976pp: 269-290pp: 269-290Samaddar, H.N.,19901990pp: 638-639pp: 638-639James, D.J.,19881988pp: 33-80pp: 33-80Roy, S.K., M.S. Islam and J. Sen,1993Elaeocarpus robustus through in vitro culture.]]>1993pp: 4444Rahman, M.M., M.N. Amin and M.A.K. Azad,20012001pp: 1010Amin, M.N.,19911991pp: 5050Bornman, C.H.,1983Picea abies in vitro.]]>57516