Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2022.211.216Cyperus esculentus) Intake in Albino Rats]]>Suleiman AliyuRamatu Muhammad UmarAbdullahi Maiwada AbubakarSalisu 52022225Background and Objective: The use of Food Frequency Questionnaire (FFQ) and 24 hrs dietary recalls remain a challenging issue in dietary assessment as a result of errors in reporting methods and dietary information available is normally biased. This study is aimed at assessing the biomarkers of tiger nut (Cyperus esculentus) intake in Albino rats. Materials and Methods: Tiger nut samples were processed using the standard technique. Twenty-five male Wistar rats weighing between 100-120 g were grouped into five with five rats per group and each group was fed with a different concentration of tiger nut juice for 28 days. The functional groups and the nutritional biomarkers in the tiger nut samples as well as serum samples of rats fed with tiger nut, were assessed using FT-IR and LC-MS, respectively. Results: FT-IR spectroscopy confirms the appearance of functional groups OH, C = O and C-O at 3291-3268, 1744-1746 and 2926-3000 cm1 bands, respectively. LC-MS analysis of serum sample of rats fed with 3 g kg1 b.wt., of whole tiger nut showed the presence of pyridoxine (m/z 170.165), pristanic acid (m/z 299.33), hydroxynicotinic acid (m/z 140.126). At 1.5 g kg1 b.wt., LC-MS analysis of the serum sample reveals the presence of proline (m/z 116.49), valine (m/z 118.033) and oxocholic acid (m/z 407.289). Conclusion: Serum proline, valine and oxocholic acids could be some of the potential biomarkers of tiger nut intake. However, more studies are needed to validate this finding.]]>Rafiq, T., S.M. Azab, K.K. Teo, L. Thabane and S.S. Anand et al.,20211223332357Freedman, L.S., N. Potischman, V. Kipnis, D. Midthune and A. Schatzkin et al.,20063510111021Scalbert, A., L. Brennan, C. Manach, C. Andres-Lacueva and L.O. Dragsted et al.,20149912861308Wishart, D.S.,200819482493Papandreou, C., M. Moré and A. Bellamine,20202020Wallace, T.C., R.L. Bailey, J.B. Blumberg, B. Burton-Freeman and C.Y.O. Chen et al.,20206021742211Eke-Ejiofor, J. and J.E. Allen,201971321Rubert, J., N. Sebastia, J.M. Soriano, C. Soler and J. Manes,2011127822826Charles, O.N., E.B. Essien and K.C. Patrick-Iwuanyawu,2018Cyperus esculentus (tiger nuts) and phoenix dactilyfera (date fruit) flour on male Albino Wistar rats.]]>710491054Ekeanyanwu, R.C. and I.C. Ononogbu,2009Cyperus esculentus L.).]]>3286292Kim, M., S. No and S.H. Yoon,2007Cyperus esculentus L.) tuber oil.]]>8410791080Barber, T.M., S. Kabisch, A.F.H. Pfeiffer and M.O. Weickert,20202020Lattimer, J.M. and M.D. Haub,2010212661289Coskuner, Y., R. Ercan, E. Karababa and A.N. Nazlican,2002Cyperus esculentus L) tubers grown in the Cukurova region of Turkey.]]>82625631Gao, Q., G. Praticò, A. Scalbert, G. Vergères and M. Kolehmainen et al.,20172017Piovesana, A., E. Rodrigues and C.P.Z. Noreña,2019Hibiscus sabdariffa L.) by HPLC-DAD-MS/MS.]]>30208217Fiehn, O., J. Kopka, P. Dormann, T. Altmann, R.N. Trethewey and L. Willmitzer,,20001811571161Trygg, J., E. Holmes and T. Lundstedt,20076469479Salisu, A.A., H. Musa, H. Abba and A.A. Kogo,20135153158Azeh, Y., Y.B. Paiko, U.U. Elele, G.O. Adesina, M.A. Mohammad, D.Z. Mohammed and A.A. Gimba,2016189103Dandashire, B., A. Magashi, B. Abdulkadir, M. Abbas, M. Goni and A. Yakubu,2019Boswellia dalzielii.]]>6183192Sunan, J., W. Thawien and S. Waranyou,201120305317Azeh, Y., Y.B. Paiko, A.Y. Abubakar, B. Garba and O.A.E. Ameh,201822713717Imo, C. and F.O. Uhegbu,2015Gongronema latifolium Benth leaf using gas chromatographic flame ionization detector.]]>16068Kumaradevan, G., R. Damodaran, P. Mani, G. Dineshkumar and T. Jayaseelan2015Clerodendrum phlomidis (L.).]]>2142148Iguchi, K., N. Okumura, S. Usui, H. Sajiki, K. Hirota and K. Hirano,2001Serenoa repens, induces apoptosis and necrosis in human prostatic LNCaP cells.]]>475965Adejuyitan, J.A., E.T. Otunola, E.A. Akande, I.F. Bolarinwa and F.M. Oladokun,2009Cyperus esculentus) sourced from a market in Ogbomoso, Nigeria.]]>35155