Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2020.35.43AlkhiderMawada Elzein Alawad ElkhidirElrashied Elimam IbrahimHanan Moawia 12020201Background and Objective: Actinokinase is a new fibrinolytic enzymes that dissolve fibrin clots. These fibrinolytic agents have potential use to treat cardiovascular diseases, such as heart attack and stroke. This study aimed to optimize physical conditions of actinokinase extracted from local isolate in batch fermentation. Materials and Methods: Isolation and identification of the isolateswere carried out using conventional technique. Enzyme was extracted and hemolytic activity of the crude enzyme and time for complete clot lysis was tested by blood agar media and test tubes containing clotted blood. Physical conditions as pH and temperature were optimized using statistical experimental design, response surface methodology (RSM). Results: About 32% of the total samples were found to be a promising enzyme producer according to hemolytic activity and large inhibition zone. Characterization of the isolates were considered as Streptomyces spp. according to microscopic and biochemical analysis. Maximum Streptomyces growth and actinokinase activity reached at 18 h of incubation. The time for complete clot lysis revealed that 20 min is the time for complete lysis of blood clot. Statistical analysis is significant with model F-value of 93.36 and R2 value of 98.53%. Optimum growth conditions of actinokinase production was at high pH of 10.6 and temperature of 36.89°C that achieve the maximum actinokinase activity to be of 47.37%. Conclusion: The optimum pH of 10.6 and optimum temperature of 36.89°C enhance three-fold actinokinase production.]]>Roth, G.A., M.D. Huffman, A.E. Moran, V. Feigin, G.V. Mensah, M. Naghavi and C.J. Murray,201513216671678Furie, B. and B.C. Furie,2008359938949Silva, G.M.M., R.P. Bezerra, J.A. Teixeira, T.S. Porto, J.L. Lima-Filho and A.L.F. Porto,2015Streptomyces sp: DPUA 1576 from Amazon lichens.]]>181619Ibrahim, H.M. and M.A.E. Ahmed,201710179185Liu, J., J. Xing, T. Chang, Z. Ma and H. Liu,2005Bacillus natto NLSSE using statistical experimental methods.]]>4027572762Deepak, V., S. Ilangovan, M.V. Sampathkumar, M.J. Victoria, S.P.B.S. Pasha, S.B.R.K. Pandian and S. Gurunathan,2010Bacillus cereus NK1 on PHB nanoparticles.]]>47297304Mahajan, P.M., S. Nayak and S.S. Lele,2012Bacillus subtilis ICTF-1: Media optimization, purification and characterization.]]>113307314Dey, S. and R.R. Chitte,200110507515Borah, D., R. Yadav, A. Sangra, L. Shahin and A.K. Chaubey,2012Bacillus subtilis, isolated from tea garden soil samples of Dibrugarh, Assam.]]>5124125Bundale, S., D. Begde, N. Nashikkar, T. Kadam and A. Upadhyay,2015Streptomyces spp. isolated from soil.]]>5441451Bezerra, M.A., R.E. Santelli, E.P. Oliveira, L.S. Villar and L.A. Escaleira,200876965977Ibrahim, H.M. and E.E. Elkhidir,20116121129Khuri, A.I.,20172017Chitte, R.R.,20132013pp: 79-88pp: 79-88You, K.M. and Y.K. Prak,2004Actinomycetes from soil.]]>10541546Hopwood, D.A., M.J. Bibb, K.F. Chater, T. Kieser and C.J. Bruton et al.,1985Streptomyces: A Laboratory Manual.]]>Pages: 356Pages: 356Williams, S.T. and T. Cross,19711971pp: 295-335pp: 295-335Collins, C.H., P.M. Lyne and J.M. Grange,19957th Edn.,Pages: 493Pages: 493Barrow, G.I. and R.K.A. Feltham,19933rd Edn.,Chitte, R.R. and S. Dey,20032003Prasad, S., R.S. Kashyap, J.Y. Deopujari, H.J. Purohit, G.M. Taori and H.F. Daginawala,2006in vitro model to study clot lysis activity of thrombolytic drugs.]]>2006Ibrahim, H.M., W.M.W. Yusoff, A.A. Hamid, R.M. Illias, O. Hassan and O. Omar,200540753758Box, G.E.P., W.G. Hunter and J.S. Hunter,1978Pages: 653Pages: 653Rama, T. and R. Vibhuti,20112795808Chitte, R.R., S.V. Deshmukh and P.P. Kanekar,2011Streptomyces sp. MCMB-379.]]>16514061413Wang, C., M. Du, D. Zheng, F. Kong, G. Zu and Y. Feng,2009Bacillus subtilis natto B-12.]]>5797229729Gesheva, V.,2009Streptomyces rimosus at conditions of nitrogen limitation.]]>15758El-Sabbagh, S.M., N.H. El-Batanony and T.A. Salem,2013Streptomyces strain isolated from Egyptian soil: Purification, characterization and evaluation of its anti-tumor. ]]>756775686Peng, Y., X. Yang and Y. Zhang,2005in vivo.]]>69126132Ju, S., Z. Cao, C. Wong, Y. Liu and M.F. Foda et al.,2019Bacillus subtilis Subsp. natto Strain WTC016 for Nattokinase Production.]]>2019Davis, L.,1993Chitte, R.R. and S. Dey,2002Streptomyces species.]]>18289294Chitte, R.R. and S. Dey,2000Streptomyces megasporus strain SD5.]]>31405410