Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2019.789.796Serratia sp. Isolated from Poultry Wastes]]>Narayanappa Yogananda MurthyVeerapura Narayanappa MurulidharaVeerapura MariswamyMahesh 82019198Background and Objectives: Keratins comprise largest complex family of cytoskeletal filament proteins found in human hairs, chicken feathers and wool. Biotechnical applications might augment the human efforts to degrade these keratins which pose a problem to environment. Present study was aimed to enumerate the potential microbial sources for production and purification of keratinase followed by characterization. Materials and Methods: Poultry waste samples (n = 20) collected and used for the isolation of organism through serial dilution. Keratin broth media employed to assess the activity using azocasein as substrate by spectrophotometry. Biochemical and morphological identifications done and confirmed as Serratia sp. by 16S rRNA sequencing. To purify the enzyme, ammonium sulphate precipitation, dialysis, ion exchange and gel filtration chromatography were carried out followed by SDS-PAGE based molecular weight determination. Results: Out of 12 organisms, one showed increased keratinase activity. The activity and yield were increased 6.4 and 16.25%, respectively followed by purification. Enzyme activity was maximum at pH 7 and temperature 37°C with keratin azure as substrate. Molecular weight of purified enzyme was shown to have 50 kDa as determined by SDS-PAGE. Conclusion: It is revealed that Serratia sp. could be a potential microbial source can be harnessed for alleviation of environmental pollution and feather waste utilization.]]>Friedrich, J., H. Gradisar, D. Mandin and J.P. Chaumont,199928127130Kornillowicz-Kowalska, T. and J. Bohacz,20113116891701Kaluzewska, M., K. Wawrzkiewicz and J. Lobarzewski,1991Streptomyces fradiae.]]>271126Kornillowicz-Kowalska, T.,1997328193Saber, W.I.A., M.M. El-Metwally and M.S. El-Hersh,2010Alternaria tenuissima and Aspergillus nidulans.]]>52135Jayalakshmi, T., P. Krishnamoorthy, G.R. Kumar and P. Sivamani,2010Actinomyces sp.]]>64548Kajiura, Y., S. Watanabe, T. Itou, K. Nakamura and A. Iida et al.,2006155438444Villa, A.L.V., M.R.S. Aragao, E.P.D. Santos, A.M. Mazotto, R.B. Zingali, E.P. de Souza and A.B. Vermelho,20132013Brebu, M. and I. Spiridon,201191288295Cai, C.G., B.G. Lou and X.D. Zheng,2008Bacillus subtilis.]]>96067Essien, J., A. Umoh, E. Akpan, S. Eduok and A. Umoiyoho,2009566169Lakshmi, P.J., C.M.K. Chitturi and V.V. Lakshmi,2013Bacillus sp.]]>2013Nayaka, S. and G.M. Vidyasagar,2013Streptomyces albus.]]>33439Rao, K.V.B. and R. Arthi,20122113126Grzywnowicz, G., J. Lobarzewski, K. Wawrzkiewicz and T. Wolski,1989Trichophyton gallinae and Trichophyton verrucosum.]]>27319328Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Laemmli, U.K.,1970227680685Govinden, G. and D. Puchooa,2012111359113600Pandian, S., J. Sundaram and P. Panchatcharam,20122274282Kunert, J.,1992Microsporum gypseum.]]>35343348Nilegaonkar, S.S., V.P. Zambare, P.P. Kanekar, P.K. Dhakephalkar and S.S. Sarnaik,2007Bacillus cereus MCM B-326.]]>9812381245Suntornsuk, W., J. Tongjun, P. Onnim, H. Oyama, K. Ratanakanokchai, T. Kusamran and K. Oda,20052111111117Cai, C. and X. Zheng,2009Bacillus subtilis KD-N2 using response surface methodology.]]>36875883Williams, C.M. and J.C.H. Shih,1989672535Barman, N.C., F.T. Zohora, K.C. Das, M.G. Mowla, N.A. Banu, M. Salimullah and A. Hashem,2017Arthrobacter sp. NFH5 isolated from soil samples.]]>2017Riessen, S. and G. Antranikian,2001Thermoanaerobacter keratinophilus sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity.]]>5399408Balaji, S., M.S. Kumar, R. Karthikeyan, R. Kumar, S. Kirubanandan, R. Sridhar and P.K. Sehgal,2008Bacillus subtilis MTCC (9102).]]>2427412745Sivakumar, T., T. Shankar and V. Ramasubramanian,2012Bacillus thuringiensis TS2 keratinase enzyme.]]>1215531557Zhang, X.,2012Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity.]]>26469Cai, C.G., J.S. Chen, J.J. Qi, Y. Yin and X.D. Zheng,2008Bacillus subtilis strain.]]>9713720Gessesse, A., R. Hatti-Kaul, B.A. Gashe and B. Mattiasson,200332519524Lin, X., D.W. Kelemen, E.S. Miller and J.C.H. Shih,1995kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1.]]>6114691474Kojima, M., M. Kanai, M. Tominaga, S. Kitazume, A. Inoube and K. Horikoshi,2006Bacillus pseudofirmus FA30-01.]]>10229235Sivakumar, T., T. Shankar, V. Thangapandian and V. Ramasubramanian,2013Bacillus cereus TS1.]]>318