Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2019.708.717SolimanMagda Ibrahim IbrahimAmira Abdallah RizkReda Mohamed NaserNashwa Saad 72019197Background and Objectives: The aquatic macrophytes (hydrophytes) can be used as biological indicators in addition to physical and chemical parameters for determining and monitoring water quality. Hence, the main aim of this study were to examine the phytoremediation of three types of hydrophytes (floating, emergent and submerged) and to investigate the effect of metals accumulation in protein and DNA of these three species. Methodology: Nine studied hydrophytes collected from two governorates El-Dakahlia and Damietta in Egypt in the spring of 2017 as follow: floating (Eichhornia crassipes, Nymphaea lotus, Pistia stratiotes), emergent (Echinochloa stagnina, Ludwigia stolonifera, Persicaria salicifolia), submerged (Ceratophyllum demersum, Myriophyllum spicatum, Potamogeton pectinatus), in addition to water and hydro soil samples for these samples. Heavy metals in plant, hydro-soil (sediments) and water samples were carried out and the concentrations in ppm were recorded. The SDS-PAGE was done using the seeds of investigated taxa. In addition, two molecular markers RAPD and ISSR using five primers in each were used based on fresh young leaves of the investigated taxa. Results: This present investigation confirmed that, emergent hydrophytes had the ability to accumulate the highest mean content of different heavy metals (Zn, Cu, Co, Cd and Pb) as 7.67, 4.06, 1.93, 2.97 and 6.65 ppm, respectively with the metals concentration uptake ordered as Zn>Pb>Cu>Cd>Co. Persicaria salicifolia collected from Kafr-Al-Tawila (Talkha), Dakahlia accumulated the highest content of mean values of heavy metals as Zn 8.40, Cu 4.62, Co 2.21, Cd 3.40 and Pb 7.73 ppm. The RAPD marker had the highest polymorphism percentage (85.71%) than ISSR (55.17%). Conclusion: This study concluded that Emergent hydrophytes can accumulate content of heavy metals higher than floating and submerged. The higher accumulation for heavy metals was recorded in Persicaria salicifolia.]]>Hoyer, M.V. and D.E. Canfield,1997Pages: 103Pages: 103Hess, A.D. and T.F. Hall,194542046Keddy, P.A.,20102nd Edn.,Pages: 497Pages: 497Tomlinson, P.B., 1986Pages: 441Pages: 441Jackson, L.J.,1998219223231Vaiopoulou, E. and P. Gikas,201246549570Khan, M.A., K.B. Marwat, B. Gul, F. Wahid, H. Khan and S. Hashim,2014Pistia stratiotes L. (Araceae): Phytochemistry, use in medicines, phytoremediation, biogas and management options.]]>46851860Cornelius, M.T.F., V. Chapla, G. Braun, M.H. Sarragiotto, J. Schirmann and C.F.A. Olguin,2016Eichhornia crassipes (Mart.) Solms.]]>8564570Thippeswamy, B.S., B. Mishra, V.P. Veerapur and G. Gupta,2011Nymphaea alba Linn. in mice as experimental models of anxiety.]]>435055Halliwell, B.,1994344721724El-Hamd, A., H. Mohamed, A.E. Mohamed, A.M. Ismail, M.A. El-Sayed and M.J. Sheded,2009Ludwigia stolonifera.]]>5306308El-Anwar, R., A.R.S. Ibrahim, K.A. Abo El-Seoud and A.M. Kabbash,2016Persicaria salicifolia Brouss. Ex Willd growing in Egypt.]]>7412Keskinkan, O.,2005Myriophyllum spicatum) in a batch system.]]>1715071517Chukina, N.V., G.G. Borisova and M.G. Maleva,2014Potamogeton alpinus Balb and Batrachium trichophyllum (Chaix) Bosch.]]>7401405Laemmli, U.K.,1970227680685Wilkinson, L.,1997Li, J., H. Yu and Y. Luan,2015121495814973Jamnicka, G., R. Hrivnak, H. Otahelova, M. Skorsepa and M. Valachovic,20062006pp: 366370Fawzy, M.A., N. El-Sayed Badr, A. El-Khatib and A. Abo-El-Kassem,201218417531771Guilizzoni, P.,19914187109Rashed, I.F., A.O. Abd-El-Nabi, M.E. El-Hemely and M.A. Khalaf,199535239252Sadia, M., S.A. Malik, M.A. Rabbani and S.R. Pearce,2009Brassica species.]]>514Mondini, L., A. Noorani and M.A. Pagnotta,200911935Sakiyama, N.S., H.C.C. Ramos, E.T. Caixeta and M.G. Pereira,2014145460