Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2019.647.653Sugarcane mosaic virus (SCMV) on Recent Sugarcane Varieties in East Java, Indonesia]]>SholehAhmil SugihartoBambang Susilo AddyHardian 72019197Background and Objective:Sugarcane mosaic virus (SCMV) is a member of the genus Potyvirus (family of Potyviridae) causing mosaic worldwide distributed. Recently, local varieties including a variety of NXI-4T (a genetically modified sugarcane) were cultivating in East Java, which has never been monitored for SCMV occurrence. This research aimed to monitor the SCMV distribution among local varieties recently cultivated in East Java. Materials and Methods: The research was performed in the 4 Sugarcane Plantation under the authority of the company of Nusantara XI in East Java between October-November, 2017. The SCMV monitoring was done by estimating the disease incidence on 10% of plants of each location. Confirmation of the virus was done by RT-PCR and western blot. Chlorophyll a, chlorophyll b and total chlorophyll were estimated by colorimetric assay at dual wavelengths of 645 and 663 nm. Results: The data showed that the incidence of mosaic disease on all varieties observed was below 20% and categorized as resistant to mosaic disease while NXI-4T (genetically modified sugarcane) had a higher incidence of a mosaic than other varieties. The additional analysis confirmed and proved that mosaic disease on all local varieties was caused by SCMV according to RT-PCR and western blot assay. The decrease of chlorophyll was the consequence of SCMV infection. Conclusion: The SCMV was more frequently occurred on genetically modified sugarcane NXI 4T than others of non-genetically modified sugarcane.]]>Huckett, B.I. and F.C. Botha,1996701113Putra, L.K., H.J. Ogle, A.P. James and P.J. Whittle,2003Sugarcane mosaic virus in sugarcane plants.]]>32305307Addy, H.S., Nurmalasari, A.H.S. Wahyudi, A. Sholeh and C. Anugrah et al.,2017Sugarcane mosaic virus (SCMV) in Indonesia.]]>2017Sugiharto, B.,20172017pp: 136-169pp: 136-169Xie, X., W. Chen, Q. Fu, P. Zhang, T. An, A. Cui and D. An,2016Sugarcane mosaic virus in Shanxi, China.]]>2016Zhao, J., X. Zhang, Y. Hong and Y. Liu,20162016Ferreira, T.H., M.S. Tsunada, D. Bassi, P. Araujo and L. Mattiello et al.,20172017Fu, W.L., S.R. Sun, H.Y. Fu, R.K. Chen, J.W. Su and S.J. Gao,2015Sugarcane streak mosaic virus.]]>2015Xu, D.L., J.W. Park, T.E. Mirkov and G.H. Zhou,200815310311039Karuppaiah, R., R. Viswanathan and V.G. Kumar,2013Sugarcane bacilliform virus isolates infecting Saccharum spp. in India.]]>46505516Darsono, N., N.N. Azizah, K.M. Putranty, N.T. Astuti, H.S. Addy, W. Darmanto and B. Sugiharto,2018Sugarcane mosaic virus and its application in the immunodiagnostic of sugarcane.]]>2018Chaves-Bedoya, G. and L.Y. Ortiz-Rojas,2012Sugarcane mosaic virus (SCMV) isolates.]]>617987Wang, Q., C. Zhang, C. Wang, Y. Qian, Z. Li, J. Hong and X. Zhou,2017Maize chlorotic mottle virus and its synergistic interaction with Sugarcane mosaic virus in maize.]]>2017Salem, R., I.A. Arif, M. Salama and G.E.H. Osman,2018Citrus psorosis virus.]]>25733738Kumar, R., A.K. Singh, D. Lavania, M.H. Siddiqui, M.H. Al-Whaibi and A. Grover,2016Vicia faba L.) plants in response to heat stress.]]>23243247Su, S., Y. Zhou, J.G. Qin, W. Yao and Z. Ma,201025531538Molazem, D., E.M. Qurbanov and S.A. Dunyamaliyev,2010Zea mays L.).]]>9319324Hartt, C.E.,197014C in sugarcane.]]>46419422Briggs, G.C., Z. Nakhid, A.T. Alleyne, J. Ayats, J.O. Despradel and W. Elibox,20149810101010Decree of Ministry of Agriculture of Indonesian,20042004Cheng, Y.J., X.P. Deng, S.S. Kwak, W. Chen and A.E. Eneji,20132013Zúñiga, G.E. and L.J. Corcuera,1987Schizaphis graminum.]]>26367369Pazarlar, S., M. Gumus and G.B. Oztekin,2013Tobacco mosaic virus infection on growth and physiological parameters in some pepper varieties (Capsicum annuum L.).]]>41427433Haider, M.S., S. Afghan, H. Riaz, M. Tahir, M.A. Javed, N. Rashid and J. Iqbal,2011Sugarcane mosaic virus (SCMV) variants from naturally infected sugarcane crop in Pakistan.]]>4311571162Viswanathan, R., R. Karuppaiah and M. Balamuralikrishnan,2010397984Gonçalves, M.C., D.M. Galdeano, I.D.G. Maia and C.M. Chagas,2011Sugarcane mosaic virus causing maize mosaic in Brazil.]]>46362369