Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2019.557.564Aspergillus niger]]>AduduJudy Atabat ArekemaseShefiat O. AbdulwaliyuIbrahim BatariMusa Latayo RaplongHelen Hoomsuk AronimoBamidele D. SaniYakubu 62019196Background and Objective: Many agro wastes have been explored for the production of citric acid. However, information on the use of corn stalk is scarcely available, hence, there is need to explore the use of corn stalk in the production of citric acid. This study is aimed at producing citric acid from corn stalk by submerged fermentation using Aspergillus niger isolated from the soil. Materials and Methods: Dried and pulverized corn stalk and A. niger obtained from soil samples were used in this study. The effects of carbon source, nitrogen source, pH, temperature, incubation time and methanol on citric acid production were studied. Results: Eight fungal isolates were characterized and isolate Asp 2 had the best citric acid production efficacy. Corn stalk powder mixed with 50% sucrose and 1 g of ammonium nitrate gave the highest yield of citric acid when compared to the other carbon and nitrogen sources. More so, pH 5, temperature of 30°C and 7th day of fermentation offered the highest yield of citric acid. Furthermore, a higher yield was obtained when 3% (v/v) methanol was added to the medium. Conclusion: Observation in this study deduced that corn stalk is a suitable and inexpensive substrate for the production of citric acid and could serve as value added agro wastes in the production of citric acid.]]>Dienye, B.N., I. Ahaotu, O.K. Agwa and N.N. Odu,2018Aspergillus niger using Chrysophyllum albidum peel.]]>9190203Dashen, M.M., S.A. Ado, J. Ameh, T. Amapu and H. Zakari,2013Aspergillus niger for citric acid production.]]>6105111Ramesh, T. and M. Kalaiselvam,2011aspergillus niger using gelidiella acerosa as a substrate.]]>51289293Yigitoglu, M.,19925100106Kareem, S.O. and R.A. Rahman,2011Aspergillus niger.]]>4384387Haq, I., S. Ali and M.A. Qadeer,2001Aspergillus niger GCMC-7.]]>453237Lotfy, W.A., K.M. Ghanem and E.R. El-Helow,2007Aspergillus niger isolate: II Optimization of process parameters through statistical experimental designs.]]>9834703477Max, B., J.M. Salgado, N. Rodríguez, S. Cortés, A. Converti and J.M. Domínguez,201041862875Maharani, V., D. Reeta, A. Sundaramanickam, S. Vijayalakshmi and T. Balasubramanian,2014Aspergillus niger from spoiled coconut.]]>3700705Alvarez-Vasquez, F., C. González-Alcón and N.V. Torres,2000Aspergillus niger: Model definition, steady-state analysis and constrained optimization of citric acid production rate.]]>7082108Adachi, O., D. Moonmangmee, H. Toyama, M. Yamada, E. Shinagawa and K. Matsushita,200360643653Kumar, D., V.K. Jain, G. Shanker and A. Srivastava, 20033817251729Ikram-ul, H., S. Ali, M.A. Qadeer and J. Iqbal,2004Aspergillus niger from cane molasses.]]>93125130Prathyusha, C., S. Hemalatha, K. Sridhar and S. Basava,20134110113Onuk, E.G., I.M. Ogara, H. Yahaya and N. Nannim,20106111IITA.,20092009AOAC.,199516th Edn.,Fawole, M.Q. and B.A. Oso,2004Barnett, H.L. and B.B. Hunter,1972pp: 62-63pp: 62-63Grigoryev, Y.,20132013Ali, S.,2004Aspergillus niger in a stirred fermentor.]]>2004Patil, N.G. and V.R. Patil,2014Aspergillus niger using sorghum.]]>5229231Khosravi-Darani, K. and A. Zoghi,20089969866993Verweij, P.E. and M.E. Brandt,20072007pp: 1802-1838pp: 1802-1838Guo, M. and J. Bi,20151038393851Elkholy, M.E.H., E.I. Hassanein, N. Edrees, W. Eleraky, M.F.A. Elgamel and D. Ibraheim,2009818341841Soccol, C.R., L.P.S. Vandenberghe, C. Rodrigues and A. Pandey,200644141149Habib, M.A., P. Abdu, C.N. Kwanashie, J. Kabir and A. Negedu,2015Aspergillus species from poultry feeds in Kaduna state, Nigeria.]]>32732Shankar, T. and T. Sivakumar,2016Aspergillus niger isolated from the leaf litter soil of Sathuragiri Hills.]]>47987El-Holi, M.A. and K. Al-Delaimy,2003Aspergillus niger.]]>2356359Pandey, A.,2003138184Grewal, H.S. and K.L. Kalra,199513209234Munshi, M.K., M.F. Hossain, R. Huque, M.M. Rahman, A. Khatun, M. Islam and M.I. Khalil,2013116Ali, S., I. Haq and M.A. Qadeer,2002Aspergillus niger.]]>323135Fawole, O.B. and S.A. Odunfa,2003Aspergillus niger.]]>52223227Adham, N.Z.,2002Aspergillus niger in beet-molasses medium.]]>8497100Lesniak, W., J. Pietkiewicz and W. Podgorski,2002Aspergillus niger.]]>2410651067Kishore, K.A., M.P. Kumar, V.R. Krishna and G.V. Reddy,2008Aspergillus niger in a batch fermentor.]]>16572577Crolla, A. and K.J. Kennedy,2001Candida lipolytica Y-1095 using n-paraffin.]]>892740Arzumanov, T.E., N.V. Shishkanova and T.V. Finogenova, 2000Yarrowia lipolytica repeat-batch culture on ethanol. ]]>53525529Nadeem, A., Q. Syed, S. Baig, M. Irfan and M. Nadeem,2010Aspergillus niger M-101 using lower alcohols.]]>35713Ganne, K.K., V.R.R.K. Dasari and H.R. Garapati,2008Aspergillus niger MTCC 282 in submerged fermentation using Colocassia antiquorum.]]>3150156Kareem, S.O., I. Akpan and O.O. Alebiowu,2010Aspergillus niger using pineapple waste.]]>6161165