Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2019.434.440in vitro Culture]]>SuaibSuaib SuaibNur Fajriani 52019195Background and Objective: In traditional plant tissue culture, sterilization medium and laboratory tools were used the autoclave procedures but this way was time consuming if compared with NaOCl system. The objectives of this observation were to shorten the sterilization procedures and to study the effect of NaOCl on in vitro callus induction (CI) and plantlet regeneration (PR) of Sugarcane plants. Materials and Methods: The laboratory tools (LT), immature leaf segments of sugarcane as explant (EP) and Murashige and Skoog basal medium (BM) were sterilized by 5.25% active chlorine (NaOCl). The different concentrations of NaOCl treatments tested were ranging from 100-500 mL L1 solute for LT and EP sterilization and 50-200 μL L1 for BM sterilization. All treatments were arranged in completely randomized design (CRD) and the percentage of LT and BM contamination, CI and PR were the observed variables. The simple mean comparison analysis was applied and to judge the best treatment of BM and EP contamination, CI and PR. Results: The best concentration of active chlorine were 300 mL L1 for LT and EP of contamination free and 100, 150 and 200 μL L1 for BM contamination-free, sugarcane CI and PR. Conclusion: Conditional protocol for non-autoclaved in vitro media, explants and laboratory tools in life span of third and callus induction and plantlets regeneration of sugarcane had successfully been achieved under active chlorine sterilization.]]>Sawant, R.A. and P.N. Tawar,2011132735Tiwari, A.K., S. Tripathi, M. Lal and S. Mishra,2012in vitro micropropagation of sugarcane.]]>14364369Sawant, R.A. and N.T. Meti,2016in vitro propagation of two varieties of sugarcane.]]>36572Teixeira, S.L., J.M. Ribeiro and M.T. Teixera,2006Ananas comosus cv Smooth cayenne) behavior.]]>86375378Teixeira, S.L.,2006in vitro banana (Musa sp.) culture.]]>471718Teixeira, S.L., J.M. Ribeiro and M.T. Teixeira,2008Eucalyptus pellita L.].]]>18185191, (In Portuguese)Brondani, G.E., L.S. de Oliveira, T. Bergonci, A.E. Brondani, F.A.M. Franca, A.L.L. da Silva and A.N. Goncalves,2013in vitro establishment of plants.]]>41257264Thepsithar, C. and A. Thongpukdee,2013Phalaenopsis protocorms.]]>78786789Deein, W., C. Thepsithar and A. Thongpukdee,2013In vitro culture medium sterilization by chemicals and essential oils without autoclaving and growth of Chrysanthemum nodes.]]>7810141017Deein, W., C. Thepsithar, A. Thongpukdee and S. Tippornwong,2013Chrysanthemum explants on MS medium sterilized by disinfectants and essential oils.]]>3609613Suaib, S., N. Arif and Ardhi,2017Capsicum frutescens L.) secara in vitro.]]>2017Suaib, S., N. Arief, G.R. Sadimantara, N.W.S. Suliartini, T.C. Rakian and Ardhi,2018In vitro seeds germination and plantlets growth of hot pepper (Capsicum frutescens L.) on non-autoclaved murashige and skoog basal medium.]]>17173181Peiris, S.E., E.D.U.D. De Silva, M. Edussuriya, A.M.U.R.K. Attanayake and B.C.N. Peiris,2012404954Rodrigues, D.T., R.F. Novais, V.H.A. Venegas, J.M.M. Dias, W.C. Otoni and E.M. de Albuquerque Villani,2013in vitro propagation of Arundina bambusifolia Lindl. and Epidendrum ibaguense Kunth.]]>60447451Bhojwani, S.S. and M.K. Razdan,1983Yeoman, M.M. and A.J. Macleod,19771977pp: 31-59pp: 31-59Murashige, T. and F. Skoog,196215473497Steel, R.G.D. and J.H. Torrie,19802nd Edn.,Pages: 633Pages: 633SAS.,2002Adilakshmi, D., M. Charumati, K. Jayachandra and P. Bebi,20152355367