Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2018.48.55SultanaAfroza SahaOtun Rahman SiddiquiAshfaqur SahaApurbha HussainMd. Saddam IslamTarequl 12018181Background and Objective: The increasing trend of Hospital acquired infections (HAIs), especially the ones caused by the multidrug resistant organisms has become a major public health concern. So that the aim of this study was to detect the dissemination of multidrug-resistant pathogenic bacteria on hands, gloves and masks of healthcare workers in some hospitals and pathology laboratories located in Noakhali and Dhaka, Bangladesh. Materials and Methods: A total of 106 fully fresh samples were collected. The samples were then subjected to various phenotypic cultural, biochemicals, antibiotic sensitivity along with molecular Polymerase Chain Reaction (PCR) analysis according to the standard procedures. Results: Approximately, 138 (57.5%) representative bacterial isolates were recovered among which the most frequently identified bacterium was E. coli 72 (52.17%) followed by Staphylococcus aureus 42 (30.43%), Salmonella typhi 14 (10.14%) and Pseudomonas aeruginosa 10 (7.25%). Among them total 33.33, 100 and 40% of Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa were pathogenic scanned through hlg, invA and OprI genes presence respectively. In contrast E. coli was tested through Congo red binding test where 36.11% were found pathogenic. The general frequency of Multiple Drug Resistance (MDR) pathogens were 79.31, 51.72, 68.97, 24.13, 6.89, 6.89, 17.24, 82.87, 24.13, 65.52 and 100% against ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, methicillin, streptomycin, tetracycline, nalidixic acid, doxycycline and imipenem consecutively. Conclusion: Healthcare personnel convey multiple drug resistance pathogenic bacteria in their protective materials which are potential source of nosocomial infections. Appropriate infection prevention measures, such as good hygiene practices and training for the healthcare workers should be taken to minimize the risks that are associated with the high rate of cross-contamination.]]>Rocha, I.V., P.D.M. Ferraz, T.G.S. de Farias and S.R. de Oliveira,201528433439Islam, T., O. Saha, S. Sultana, M. Hridoy, M. Hasan, S. Marzan and M.M. Rahman,2017Pseudomonas aeruginosa and Staphylococcus aureus in Bangladesh.]]>28897Hidron, A.I., J.R. Edwards, J. Patel, T.C. Horan and D.M. Sievert et al.,2008299961011Fazeli, N. and H. Momtaz,2014Pseudomonas aeruginosa isolated from Iranian hospital infections.]]>2014Brady, R.R., S.F. Fraser, M.G. Dunlop, S. Paterson-Brown and A.P. Gibb,200766397398Ducel, G., J. Fabry and L. Nicolle,20022nd Edn.,Pages: 64Pages: 64Pal, S., D. Juyal, S. Adekhandi, M. Sharma and R. Prakash et al.,20152015Diaz, M.H., C. Silkaitis, M. Malczynski, G. Noskin, J. Warren and T. Zembower,2008296365CDC.,20032003Lee, T.B., O.G. Baker, J.T. Lee, W.E. Scheckler, L. Steele and C.E. Laxton,199826277288Rimi, N.A., R. Sultana, S.P. Luby, M. Saiful Islam and M. Uddin et al.,20142014Adhikari, U.K., O. Saha, M. Ahmmed, A. Sultana, F. Rahman, K. Islam and M. Hasan,2016in silico approach.]]>5770779Momtaz, S., O. Saha, M.K. Usha, M. Sultana and M.A. Hossain,2018Salmonella spp. in poultry slaughter-house in Bangladesh.]]>4506515Samanipour, A., S. Dashti-Khavidaki, M.R. Abbasi and A. Abdollahi,201654351Buddingh, G.J.,19748th Edn.,Rahman, A., M.S. Islam, O. Saha and T.C. Saha,2016448593Sharma, K.K., S.S. Soni, S. Meharchandani,2006Escherichia coli-short communication..]]>76363366Rahn, K., S.A. De Grandis, R.C. Clarke, S.A. McEwen and J.E. Galan et al.,1992Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella.]]>6271279Kumar, J.D., Y.K. Negi, A. Gaur and D. Khanna,2009Staphylococcus aureus isolated from paper currency.]]>13e450e455Nontongana, N., T. Sibanda, E. Ngwenya and A.I. Okoh,2014Escherichia coli pathotypes isolated from the Kat River and the Fort Beaufort abstraction water.]]>1182138227Prager, R., S. Mirold, E. Tietze, U. Strutz and B. Knuppel et al.,2000Salmonella enterica.]]>290605617Ustun, C. and M. Cihangiroglu,20129538542Lakdawala, N., J. Pham, M. Shah and J. Holton,20113211031108Morgan, D.J., E. Rogawski, K.A. Thom, J.K. Johnson and E.N. Perencevich et al.,20124010451051Ng, L.S.Y., W.T. Teh, S.K. Ng, L.C. Eng and T.Y. Tan,201178231233Weber, R.L., P.D. Khan, R.C. Fader and R.A. Weber,201280252254Chaka, T.E., G.M. Misgana, B.W. Feye and R.T. Kassa,20162016Hall, M., U. Trivedi, K. Rumbaugh and S. Dissanaike,20142310Luksamijarulkul, P., N. Aiempradit and P. Vatanasomboon,201429346350Kim, S.J. and H.J. Jeong,20132013