Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2015.355.366Mentha Species and Varieties under Sandy
Soil Conditions]]>YousefRabia M.M. Abu El-LeelOmneya F. MohamedSayed Y. 22015152The present study was
conducted with the objective of finding out the concordance between growth and
yield production, biochemical (the volatile oil) and molecular genetic (RAPD and
ISSR-PCR) characteristics of three species of genus Mentha (Mentha
viridis; Mentha piperita; Mentha aquatica) and two subspecies
(Mentha spicata var. Morocana; Mentha spicata var.
Longofolia). Mentha species and subspecies were collected from
the Experimental Farm of the El-Kasaseen Research Station, Horticulture Research
Institute, A.R.C. The highest oil percentage of fresh and dry Mentha
herb obtained of Menthaaquatica (0.46, 2.11), respectively
and Mentha piperita (0.40, 2.09), respectively in the second season.
While the production yield of herb/fed. Mentha piperita recorded (3.148,
3.047 t) in the first and second season,respectively while Mentha aquatica
recorded (1.513, 1.418 t) in the first and second season, respectively.
GC analysis of the volatile oil prepared by hydrodistillation from aerial parts
of spearmint, the major constituents of the oils in Mentha viridis were
limonene (15.73%) and carvone (75%) while the major ones in Mentha piperita
oil were limonene (10.57%), 1, 8-cineol (8%), menthone (14.74%), isomenthone (8.34%), menthol
(13%) and -caryophyllene (28.85%) which was the main one in Mentha spicata
var. Longofolia oil by (46.75%) and (70.3%) in Mentha spicata var.
Morocana. Menthyl acetate (86.11%) was the major constituents of Mentha
aquatica oil. For molecular study Random Amplified Polymorphic DNA (RAPD)
was performed which was efficient in detecting polymorphism and genetic variation
within and between Mentha species and varieties. In RAPD analysis, 5
selected primers displayed a total of 71 amplified fragments, in which 55 (77.46%)
were polymorphic fragments. The number of total amplified fragments scored per
primer ranged from 7 (primer OP-E19) to 26 (primer OP-Ax06). Thirty-three out
of 71 RAPD-PCR fragments were found tobe useful as cultivar specific markers.
The largest number of RAPD-PCR markers was scored for Mentha viridis
(9 markers) while the lowest (5 markers) was scored for Mentha aquatica
and Mentha spicata var. Morocana. In the meantime, the largest
number of RAPD-PCR cultivar-specific markers was generated by primer OPAX-6 (12
markers) while the lowest number of RAPD-PCR specific markers (2 markers) was
generated by primer OP-E19. In ISSR analysis, 5 of the tested ISSR primers generated
variable banding patterns. A total of 50 out of 64 ISSR fragments were polymorphic.
Twenty-six DNA amplified fragments were considered as cultivar-specific markers.
Genetic similarities among the Mentha species/varieties were estimated
according to the RAPD and ISSR data. Results of the combination of the banding
patterns of the two techniques (RAPD and ISSR) data exhibited that the most two
closely related cultivars were Mentha piperita and Mentha spicata
var. Morocana with the highest similarity index (1.00). On the other
hand, the two most distantly related cultivars were Mentha aquatica and
Mentha spicata var. Longofolia with low similarity index (0.00).
In conclusion, RAPD and ISSR polymorphisms could be used as efficient tools for
the detection of similarities and phylogenetic relationships of the studied genotypes
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