Journal of Applied Sciences1812-56541812-5662Asian Network for Scientific Information10.3923/jas.2012.1909.1916ReanprayoonPradub PathomsiriwongWattanachai 1220121218The objective of this study was to screen, identify and characterize
of cellulolytic fungi from various soil management fields; organic, young organic,
semi-chemical and chemical soil from Surin rice fields, Thailand. Fungi from
various type of soils were isolated by dilution plating technique on Reese minimal
medium supplemented with cellulose powder and rice straw then incubated at 25°C
for 3-7 days. The isolated fungi were screened and identified using slide culture
technique. The enzymatic activities were assessed by qualitative method for
cellulase, xylanase, peroxidase and laccase activities. Two-hundred and fifty-eight
fungi isolates found in Surin rice fields belonging to the genus Penicillium
(5 species), Paecilomyces (4 species), Aspergillus (3 species),
Acremonium (2 species), Chaetomium (2 species), Alternaria
(1 species), Bipolaris (1 species), Curvularia (1 species),
Fusarium (1 species), Humicola (1 species), Mucor (1 species),
Nigrospora (1 species), Phoma (1 species), Pyrenochaeta
(1 species), Pythium (1 species), Rhizopus (1 species), Sporotrichum
(1 species) and Trichoderma (1 species). Out of 29 fungal species clearly
showed different in enzymatic activities. Most tropical soil fungi had ability
to produce cellulase, xylanase, laccase and peroxidase, respectively. The highest
capacity was found only in cellulase. Aspergillus niger, Aspergillus
sp., Chaetomium murorum and Trichoderma sp. showed the highest
potential to produce cellulase. Eleven species of soil fungi showed high capacity
in xylanase activity. For laccase and peroxidase activity, there were found
in 2 species. The results also revealed that only ten showed highest carboxymethyl
cellulase, xylanase, peroxidase and laccase activities by qualitative screening
method for enzymatic assay. They were identified as Aspergillus niger,
Acremonium sp., Aspergillus sp., Chaetomium murorum, Humicola
grisea, Mucor sp., Paecilomyces victoriae, Penicillium
janthinellum, Penicillium lanosum and Trichoderma sp. These
tropical soil fungi will be beneficial to use for biodegradation and decomposition
of agricultural residues, especially rice straw.]]>Abdulla, H.M. and S. El-Shatoury,200727850853Alves-Prado ,H.F., F.C.Pavezzi, R.S.R. Leite, V.M. De Oliveira, L.D. Sette and R. Dasilva,2010161333346Arenz, B.E. and R.A. Blanchette,201143308315Bansal, N., R. Tewari, R. Soni and S.K. Soni,2012Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues.]]>2012Barnett, H.L. and B.B. Hunter,19994th Edn.,Bastias, B.A., I.C. Anderson, J.I. Rangel-Castro, P.I. Parkin, J.I. Prosser and J.W.G. Cairney,200913C from cellulose by forest soil fungi as determined by RNA stable isotope probing.]]>41467472Belal, E.B. and M.E. El-Mahrouk,20106710811089Braun, B., U. Bockelmann, E. Grohmann and U. Szewzyk,2010158343351Bulluck, L.R., M. Brosius, G.K. Evanylo and J.B. Ristaino,200219147160Cabello, M. and A. Arambarri,2002Celtis tala and Scutia buxifolia forests in the Eastern Buenos Aires province (Argentina).]]>157115125Chandra, M.S., B. Viswanath and B.R. Reddy,2007Aspergillus niger.]]>47323328Connell, L., R. Redman, S. Craig and R. Rodriguez,20063830833094De Castro, A.M., T.V. De Andrea, L.R. Castilho and D.M.G. Freire,201016216121625Dhouib, A., M. Hamza, H. Zouari, T. Mechichi and R. Hmidi et al.,20052114151423Donner, M., J. Atehnkeng, R.A. Sikora, R. Bandyopadhyay and P.J. Cotty,2009Aspergillus section Flavi in soils of maize fields in three agroecological zones of Nigeria.]]>413744Gadde, B., S. Bonnet, C. Menke and S. Garivait,200915715541558Gadde, B., C. Menke and R. Wassmann,20093315321546Gochev, V.K. and A.I. Krastanov,2007Trichoderma Spp.]]>13171176Grishkan, I., A. Tsatskin and E. Nevo,200844180190Hart, T.D., F.A.A.M. De Leij, G. Kinsey, J. Kelley and J.M. Lynch,200218471480Jaime-Garcia, R. and P.J. Cotty,2010Aspergillus flavus in soil.]]>4218421847Jin, S. and H. Chen,200726207211Kausar, H., M. Sariah, H.M. Saud, M.Z. Alam and M.R. Ismail,201064594600Kim, M.N., A.R. Lee, J.S. Yoon and I.J. Chin,2000® and Mater-Bi® by fungi isolated from soils.]]>3616771685Oanh, N.T.K., B.T. Ly, D. Tipayarom, B.R. Manandhar, P. Prapat, C.D. Simpson and L.J.S. Liu,201145493502Kuhad, R.C., A. Singh and K.E.L. Eriksson,19975745125Kumar, A., S. Gaind and L. Nain,200819395402Liang, Y.S., X.Z. Yuan, G.M. Zeng, C.L. Hu and H. Zhong et al.,2010Phanerochaete chrysosporium in the presence of dirhamnolipid.]]>21615624Liu, B., D. Glenn and K. Buckley,2008Trichoderma communities in soils from organic, sustainable and conventional farms and their relation with Southern blight of tomato.]]>4011241136Meriles, J.M., S.V. Gil, C. Conforto, G. Figoni, E. Lovera, G.J. March and C.A. Guzman,2009103271281Minamiyama, H., M. Shimizu, H. Kunoh, T. Furumai, Y. Igarashi, H. Onaka and R. Yoshida,2003Streptomyces galbus on rhododendron leaves and its production of cell wall-degrading enzymes.]]>696570Parr, J.F., R.I. Papendick, S.B. Hornick and R.E. Meyer, 19927511Pointing, S.B.,199921733Qiao, H., C. Tian, Y. Luo, J. Sun and X. Feng,20083347351Reanprayoon, P. and W. Yoonaiwong,2012284960Rodriguez, A., A. Moral, R. Sanchez, A. Requejo and L. Jimenez,200910048634866Shukla, N., R.P. Awasthi, L. Rawat and J. Kumar,2012Oryza sativa L.) as influenced by Trichoderma harzianum under drought stress.]]>547888Stepanova, E.V., O.V. Koroleva, L.G. Vasilchenko, K.N. Karapetyan and E.O. Landesman et al.,2003396574Tortella, G.R., O. Rubilar, L. Gianfreda, E. Valenzuela and M.C. Diez,20082428052818Toyama, H. and N. Toyama,2001Trichoderma.]]>91-93787790Wang, Y., J. Xu, J. Shen, Y. Luo, S. Scheu and X. Ke,20101077179Watanabe, T.,2002Trichoderma harzianum: Pictorial Atlas of Soil and Seed Fungi Morphologies of Cultured Fungi and Key to Species.]]>2nd Edn.,Whitelaw-Weckert, M.A., L. Rahman, R.J. Hutton and N. Coombes,200736224232Xu, C., M. Long, X. Wu, H. Xu, Z. Chen, F. Zhang and L. Xu,2006Aspergillus glaucus XC9.]]>13540Xu, J. and Q. Yang,2010Streptomyces griseorubens C-5.]]>21107116Xu, Z.H., Y.L. Bai, X. Xu, J.S. Shi and W.Y. Tao,2005Pseudomonas sp. WLUN024 with wheat bran as the main substrate.]]>21575581Yang, S.S., H.Y. Fan, C.K. Yang and I.C. Lin,20035214891498Yu, H., G. Zeng, H. Huang, X. Xi, R. Wang and D. Huang et al.,200718793802Porras, M., C. Barrau and F. Romero,2007Trichoderma on strawberry production.]]>26782787