Information Technology Journal1812-56381812-5646Asian Network for Scientific Information10.3923/itj.2006.94.107BrittoA. Prabhu .RavindranG .1200651In this study, the suitability of the characterized parameters governing the DCT based GVF active contour formulation (curve evolution method) as standardized values for boundary mapping chromosome spread images was investigated as an evaluation of standardization of the boundary mapping technique. It was found experimentally that a unique set of parameter values of the technique is required for boundary mapping every chromosome image. Characterization studies showed that each parameter has an optimal range of values within which good boundary mapping results can be obtained for various chromosomes in similar class of images. Statistical testing validates the experimental results of characterization. Standardization of characterized parameters was then carried out using a different dataset comprising of similar class of chromosome spread images so that the standardization testing is independent of dataset.]]>Britto, A.P. and G. Ravindran,2006613511361Kass, M., A. Witkin and D. Terzopoulos,19881321331Rueckert, D.,19971997Xu, C. and J.L. Prince,19971997pp: 6671Leroy, B., I. Herlin and L.D. Cohen,19961996pp: 5865Cohen, L.D.,199153211218Cohen, L.D. and I. Cohen,19931511311147Davatzikos, C. and J. Prince,1995146580Davatzikos, C. and J.L. Prince,19941994pp: 581587Abrantes, A.J. and J.S. Marques,1996515071521Prince, J.L. and C. Xu,19961996pp: 3031Xu, C. and J.L. Prince,2000pp: 159-169pp: 159-169Xu, C. and J.L. Prince,19987359369Tang, J. and S.T. Acton,20042004pp: 157161