International Journal of Zoological Research1811-97781811-9786Academic Journals Inc.10.3923/ijzr.2017.120.124Oreochromis mossambicus]]>RajJustin JosephBaby 32017133Background and Objective: Acetamiprid is a neonicotinoid pesticide and it is used by the farmers to protect their crops. This pesticide residue reaches to the aquatic ecosystem by many ways and affects the aquatic fauna. The aim of the study was to analyze the effect of pesticide-acetamiprid on electrophoretic protein patterns of liver, brain and gill tissues of Oreochromis mossambicus (O. mossambicus). Materials and Methods: Lethal concentration (LC50) of acetamiprid for O. mossambicus has been calculated by probit analysis. Sublethal or safe level concentrations were derived from 96 h LC50. In the present study 1/15 of the 96 h LC50 were selected as sublethal concentration and the fishes were exposed to this concentration for a period of 7 and 14 days. Results: The electrophoretogram of 7th and 14th day exposure represents the decrease in the intensity of liver, brain and gill protein subunits and increase in protein bands due to acetamiprid toxicity, when compared to control. Studies revealed that due to the toxic effect of the pesticides the protein bands get denatured, as a result there was a decrease in the intensity of protein bands and there was an increase in new protein bands due to the enhancement of stress proteins. Conclusion: The alterations in electrophoretic protein patterns observed in various tissues of the experimental fish could serve as sensitive biochemical indicators of acetamiprid pollution, which might help in aquatic management. It may be concluded that long term exposure to acetamiprid creates a continuous health hazard for Oreochromis mossambicus.]]>Malla, F.A., G. Sharma and S. Singh,2009Channa punctatus (Bloch.).]]>15455Joseph, B. and S.J. Raj,20117212222Aktar, M.W., D. Sengupta and A. Chowdhury,20092112Muthukumaravel, K., O. Sathick and S. Raveendran,2013Oreochromis mossambicus (peters).]]>18085Raj, S.J. and B. Joseph,2015Oreochromis mossambicus.]]>11222227Banaee, M., A.R. Mirvagefei, G.R. Rafei and B.M. Amiri,20082189198Ganesan, R.M., S.R.D. Jebakumar and J. Jayaraman,1989Oreochromis mossambicus.]]>985155Rita, J.J.A. and M.C.J. Milton,2006Oreochromis mossambicus (Peters).]]>25655658Bradley, B.P., E.A. Shrader, D.G. Kimmel and J.C. Meiller,200254373377Huang, Q.Y. and H.Q. Huang,2011Danio rerio) brain exposed to methyl parathion.]]>1137433756Huang, Q., J. Zhang, S. Peng, M. Du and S. Ow et al.,20143413421351Dowling, V.A. and D. Sheehan,2006655975604Sharma, G., F.A. Malla and S. Singh,2009Channa punctatus (Bloch).]]>12527Chen, H.B. and H.Q. Huang,2011Sparus latus liver.]]>30800806Nesatyy, V.J. and M.J.F. Suter,20074168916900Tripathi, G. and S. Shukla,199035264Muthukumaravel, K., P. Kumarasamy, A. Amsath and G. Paulraj,2007Oreochromis mossambicus.]]>4284286Manna, G.K. and P.K. Mukherjee,19865225235El-Sherif, M.S., M.T. Ahmad, M.A. El-Danasoury and N.H.K. El-Nwishy,2009O. niloticus).]]>4169177Finney, D.J.,19713rd Edn.,Pages: 318Pages: 318APHA.,200521st Edn.,Pages: 1200Pages: 1200Laemmli, U.K.,1970227680685Frigo, D.E., Y. Tang, B.S. Beckman, A.B. Scandurro, J. Alam, M.E. Burow and J.A. McLachlan,200425249261Cheshenko, K., F. Pakdel, H. Segner, O. Kah and R.I.L. Eggen,20081553162Senturk, M., S.B. Ceyhun, O. Erdogan and O.I. Kufrevioglu,2009In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes.]]>959599Agrahari, S. and K. Gopal,2009Channa punctatus.]]>9459Sahai, S.,19901990pp: 63-72pp: 63-72Ravinder, V., N. Suryanarayana and G. Narayana,1988Clarias batrachus.]]>6512Rita, A. and M.C.J. Milton,2007Oreochromis mossaaibiclis (Peters).]]>94952Mukhopadhyay, P.K. and P.V. Dehadrai,1980Clarias batrachus (Linn.) under sublethal malathion exposure.]]>18400404Kumar, K.B. and K.S. Devi,199234408410