International Journal of Zoological Research1811-97781811-9786Academic Journals Inc.10.3923/ijzr.2011.310.319SujathaS. JeyaparvathyS. PriyaM. Jasmine4201174The objective of this study was to determine the bacterial pathogen and gene content of the Etroplus suratensis (pathogenic shrimp). PCR-based subtractive hybridization method detected with the help of Escherichia coli on shrimp liver and granulose cells for efficiently detecting the DNAs and applies in to liver pathogen. Seventeen DNAs specific to a mono-key colonizing strain (J140) were obtained by subtractive hybridization against an unrelated strain whose genome has been fully sequenced (ABO18799). Among the seventeen different clones 14 were unique and other three numbers were each represented at twice. Nine of the clones DNAs were found by sequencing to be absent or very divergent from those in the same direction on disappeared blank region of this liver and granulosa cells. Nine others had no database match with proteins of assigned function. PCR tests of 13 unrelated E.coli strains by using primer specific for 12 subtracted clones and complementary southern hybridizations indicated that these DNAs are highly polymorphic in the E. coli population with each strain yielding a different pattern of gene-specific PCR amplification. When described about this Polymorphic immune response gene, it helps to identify the previously unknown virulent genes in the pathogens and it provides a new insight of microbial genetic diversity and evolution.]]>Agouz, H.M. and W. Anwer,2011® and Myco-ad® on the growth performance of common carp (Cyprinus carpio) fed a mycotoxin contaminated aquafeed.]]>6334345Alonson, M. and J. Leong,200247480Aranishi, F. and N. Mano,2000108789Aziz, D., S.S. Siraj, A. Arshad, S.M.N. Amin and S.A. Harmin,2010Acetes japonicus (Decapoda: Sergestidae) using RAPD technique.]]>10355361Bayne, C.J., L. Gerwick, K. Fujiki, M. Nakao and T. Yano,2001Oncorhynus mykiss, by means of suppression subtractive hybridization.]]>25205217Bayne, C.J. and L. Gerwick,200125725743Bobe, J. and F.W. Goetz,2000Salvelinus fontinalis) ovary at the completion of ovulation.]]>62420426Bobe, J. and F.W. Goetz,200060312318Himmelreich, R., H. Plagens, H. Hilbert, B. Reiner and R. Herrmann,1997Mycoplasma Pneumoniae and Mycoplasma Genitalium.]]>25701712Brown, P.K. and R. Curtiss,1996Escherichia coli strain.]]>931114911154Coffey, T.J., C.G. Dowson, M. Daniels and B.G. Spratt,1995Pneumococci.]]>12934Douglas, S.E., S.C.M. Tsoi, S. Penny, K. Melville, M.E. Reith and K.V. Evart,20032003Ellis, A.E.,200125827839Fujiki, K., D. Shin, M. Nakao and C.J. Bayne,2000Oncorhynchus mykiss.]]>24594607Gerwick, L., R. Steinhauer, S. Lapatra, T. Sandell, J. Ortuno, N. Hajiseyedjavadi and C.J. Bayne,2002Oncorhynchus mykiss) plasma proteins to viral, bacterial and fungal inflammatory agents.]]>12229242Gorisman, E.A. and H. Ochman,199687791794Hedge, P., R. Qi, K. Abernathy, C. Gasy and S. Dharap et al.,200029548550Janeway, C.A., P. Travers, M. Walport and M. Shlomchik,20015th Edn.,Jorgensen, J.B., H. Lunde, L. Jensen, A.S. Whitehead and B. Robertson,200024553563Kaattari, S.L. and J.D. Piganelli,19961996pp: 207-243pp: 207-243Kanamori, A.,2000553136Lee, P.H. and F.W. Goetz,1998Salvelinus fontinalis).]]>49112118Lund, V. and J.A. Olafsen,199822185194Pan, D., N. He, Z. Yang, H. Liu and X. Xu,2005Penaeus japonicus) by suppression subtractive hybridization.]]>29103112Rane, P.P. and S.S. Barve,20117190200Savan, R. and M. Sakai,2002Cyprinus carpio L., head kidney cells after stimulation by two mitogens, lipopolysaccharide and concanavalin-A.]]>1317182Seifert, H.S.,1996Gonococcal pilus phase and antigenic variation.]]>21433440Shike, H., X. Lauth, M.E. Westerman, V.E. Ostland and J.M. Carlberg et al.,200226922322237Sriputhorn, K. and L. Sanoamuang,2011Streptocephalus sirindhornae) as live feed improve growth and carotenoid contents of giant freshwater prawn Macrobrachium rosenbergii.]]>7138146Tiffoche, C., A. Chesnel, P. Jego and J.P. Le Pennec,1993Pleurodeles lectin.]]>213901907Tinsley, C.R. and X. Nassif,1996Neisseria meningitidis and Neisseria gonorrhoeae: Two closely related bacteria expressing two different pathogenicities.]]>931110911114Van Hal, N.LW., O. Vorst, A.M.M.L. van Houwelingen, E.J. Kok and A. Peijnenburg et al.,200078271280Velculescu, V.E., L. Zhang, B. Vogelstein and K.W. Kinzler,1995270484487Waldor, M.K. and J.J. Mekalamos,199621219101914Yotsu-Yamashita, M., A. Sugimoto, T. Terakawa, Y. Shoji, T. Miyazawa and T. Yasumoto,200126859375946James, R., N. Thampuran, K.V. Lalitha, L.A. Rajan and T.C. Joseph,2010Fenneropenaeus indicus by suppression subtractive hybridization.]]>29884889Thangaraj, M., V. Prem, T. Ramesh and A.P. Lipton,20113269274