International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2017.14.28Tospovirus by Thrips tabaci L. to Onion Plants in Egypt]]>El-ShazlyManal A. Abd El-WahabA.S. 12017131Background and Objective: Iris Yellow Spot Virus (IYSV) genus Tospovirus, family Bunyaviridae is one of the most important viruses affecting onion crops worldwide. In Egypt, IYSV was recorded for the first time from onion since, 2004. Thrips tabaci L. is the mainly vector of this virus. The present study was conducted to test the effect of jojoba seeds extract and riboflavin on decrease the transmission rate of IYSV by Thrips. Materials and Methods: The virus has been isolated from naturally infected onion plants grown in the experimental farm, Faculty of Agriculture, Cairo University, Giza. It was identified by mechanically and T. tabaci transmissibility, symptomatology, serology and transmission electron microscopy. Two pots experiments were conducted in 2013-2014 seasons to study the effect of spraying with jojoba seeds extract at 100, 500 and 1000 Fg mL1 and riboflavin at 0.5, 1.0 and 2.5 mM to inhibit infection with IYSV in onion plants (cv. Giza 20) exposed to Thrips. Data were subjected to analysis of variance and the means were compared using the Least Significant Difference (LSD) test at the 0.05 levels. Results: All treatments with jojoba seeds extract caused virus inhibition through reduction in the transmission rates of IYSV by T. tabaci and riboflavin through induced resistance against virus infection when plants were sprayed before the virus transmission. The highest concentration of jojoba seed extract (1000 Fg mL1) and riboflavin at (1 mM) were the best treatments which resulted in the highest reduction of viral infection (58 and 36%), respectively. 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