International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2014.46.54Avian
influenza H5N1 Infected Broiler Flock in Egypt]]>HusseinH.A. EmaraM.M. RohaimM.A. 12014101In the early months of 2011, several devastating Newcastle disease outbreaks
occurred in Egypt affecting commercial poultry. Both vaccinated and unvaccinated
flocks were affected. In the present study, we characterized field isolate of
NDV from a broiler flock coinfected with Avian influenza H5N1 showing
characteristic clinical signs, post mortem gross lesions and was positive to
A.I H5N1 and NDV by RT-PCR. The fusion protein gene of the Newcastle Disease
Virus (NDV) isolate was partially amplified by RT-PCR, directly sequenced. The
obtained sequence was submitted to GeneBank with the accession No. JX885868
(NDV/chicken/VRLCU138/Egypt/2012). Sequence was aligned and compared with a
representative NDV isolates of different genotypes using NCBI BLAST. The F protein
cleavage site sequence is a well-characterized determinant of NDV pathogenicity
in chickens. The NDV isolate was found to have the motif 112RRQKRF117 which
is indicative of the velogenic nature of this NDV isolate. Phylogenetic analysis
revealed that then isolated NDV is belonging to subgenotype VIId and in close
range to other Chinese VIId NDV isolates based on the high nucleotide similarity
between them which indicate that genotype VIId is circulating between chicken
flocks. This study reports the characterization of Newcastle Disease Virus genotype
VIId in broiler chickens coinfected with Avian influenza H5N1
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