International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2014.136.142El-HaigM. SelimA. ShahiraA. 22014102Lumpy Skin Disease (LSD) is one of the major threats of cattle stock industry
in Egypt. There is many confusion in laboratory diagnosis of lumpy skin disease.
Specific Lumpy Skin Disease Virus (LSDV) primers were used in Polymerase Chain
Reaction assay (PCR) on field samples of skin biopsies and EDTA blood from clinically
infected cattle located in Ismaillia province, Egypt to assess their efficiency
for LSD diagnosis. The specificity of the primers was confirmed by using other
Capripox viruses (CaPVs) such as vaccinal strain of sheep poxvirus (SPPV)
and local adapted tissue culture LSDV (Ismaillia 88). Specific LSDV primers
were successfully and specifically detected the LSDV in 100% of skin biopsies
and 40% of EDTA blood samples and did not able to detect SPPV. Therefore, specific
PCR is a valuable technique for accurate diagnosis of LSD without any confusion
with other related viruses and differentiate LSDV from SPPV and must be used
as routine diagnostic test for LSD. Detection of LSDV in field samples by using
PCR and Dot Blot Hybridization (DBH) were compared. The results revealed that
both PCR and DBH detected LSDV in 100% skin biopsies while detection rates in
EDTA blood were 40 and 30% using PCR and DBH, respectively.]]>Aspden, K., J.A. Passmore, F. Tiedt and A.L. Williamson,2003Capripox virus, as a replication-deficient vaccine vector. ]]>8419851996Awad, W.S., A.K. Ibrahim, K. Mahran, K.M. Fararh and M.I. Abdel Moniem,201042777783Barnard, B. J., E. Munz, K. Dumbell and L. Prozesky,19941994pp: 604-612pp: 604-612Bowden, T.R., B.E. Coupar, S.L. Babiuk, J.R. White and V. Boyd et al., 20091611929Buller, R.M., B.M. Arif, D.N. Black, K.R. Dumbell and J.J. Esposito et al.,20052005Burdin, M.L. and J. Prydie,1959183949950Capstick, P.B.,195975162Carn, V.M. and R.P. Kitching,1995140503513Davies, F.G.,1991Capripox virus disease of cattle.]]>147489502El-Kholy, A.A., H.M.T. Soliman and K.A. Abdelrahman,200811293302Hamoda, F.K., E.A. Aboul-Soud, M.S. Magda, M.A. Shahein, A. Michael and A.M. Daoud,2002625183199Hosamani, M., B. Mondal, P.A. Tembhurne, S.K. Bandyopadhyay, R.K. Singh and T.J. Rasool,2004297380House, J.A., T.M. Wilson, S. El Nakashly, I.A. Karim and I. Ismail et al.,19902111115Ireland, D.C. and Y.S. Binepal,19987417Kitching, R.P.,2003114161167Kitching, R.P. and V.M. Carn,20082008Lamien, C.E., C. Le Goff, R. Silber, D.B. Wallace and V. Gulyaz et al.,2011Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: Development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus.]]>1493039Lamien, C.E., M. Lelenta, W. Goger, R. Silber and E. Tuppurainen et al.,2011171134140Southern, E.M.,197598503517Stram, Y., L. Kuznetzova, O. Friedgut, B. Gelman, H. Yadin and M. Rubinstein-Guini,2008151225229Tulman, E.R., C.L. Afonso, Z. Lu, L. Zsak and J.H. Sur et al.,20027660546061Tuppurainen, E.S.M. and C.A.L. Oura,2012594048Tuppurainen, E.S., E.H. Venter and J.A. Coetzer,200572153164Van Rooyen, P.J., E.K. Munz and K.E. Weiss,196936165174Weiss, K.E.,19683111131OIE Manual,20102010