International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2012.61.70SolimanAhmed M. MahmoudSabry Y.M. DawoodRehab A. 1201281Garlic samples showing symptoms of Onion Yellow Dwarf Virus (OYDV) were obtained from previous study and tested by indirect-enzyme linked immunosorbent assay (I-ELISA), transmitted to Chenopodium amaranticolor and then confirmed by immunosorbent electron microscopy (ISEM) for the presence of OYDV. PCR primers were used to amplify about 1.1 kb fragment from the viral genome using RT-PCR from infected garlic plants, such fragment were not obtained from healthy- looking plants and/or virus-free seedlings of shoot-tips. The amplified products of OYDV was cloned into pGEM®-T Easy vector and transformed into Escherichia coli strain DH5a. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The sequence analysis showed that; nucleotide sequence of OYDV-EG [Egyptian isolate (HM473189)] had 82-96% similarity compared with ten reported OYDV isolates. Thus, a method of identification and detection by RT-PCR of OYDV was established. This study also aimed to obtain OYDV-free plants from infected garlic plants using cloves subjected to electrotherapy, thermotherapy, chemotherapy or meristematic dissection followed by in vitro culture. A combination treatment with electro- and chemotherapy (15 mA/10 min + 20 mg L-1 virazol) was found to more effective on viral elimination and survival of explants. ELISA tests showed that 85% of the plantlets that survived were OYDV-negative.]]>AVRDC,19971997Black, J.D., F.R. Forsyth, D.S. Fensom and R.B. Ross,19714918091815Chen, J., H.Y. Zheng, J.F. Antoniw, M.J. Adams, J.P. Chen and L. Lin,2004149435445Conci, V.C., M. Helguera and S.F. Nome,1999Onion yellow dwarf virus from onion and garlic in Argentina.]]>247385Dhital, S.P., H.T. Lim and B.P. Sharma,2008in vitro plantlets of potato (Solanun tuberosum L.).]]>495257Dovas, C. I., E. Hatziloukas, R. Salomon, E. Barg, Y. Shiboleth and N.I. Katis,2001Alliums sp. in Greece.]]>107677684Dovas, C.I., E. Hatziloukas, R. Salomon, E. Barg, Y. Shiboleth and N.I. Katis,2001Allium spp.]]>149731737El-Kewey, S.A., R.A. Omar, S.A. Sidaros and Samaa Abd El-khalik,20041169178FAO/IPGRI,1997Allium spp. Technical guidelines for the safe movement of germplasm.]]>1997Gibbs, A. and A. Mackenzie,199763916Guta, I.C., E. Buciumeanu, R.N. Cheorghe and A. Teodorescu,2010V. vinifera L. cv. Ranai Magaraci.]]>157278Hernandez, R., J. Fontanella, J.C. Noa, T. Pichardo, R. Manzo and H. Cardenas,1999Allium sativum L.).]]>166871Hwang, H. Y. and Y.B. Lee,2008Allium sativium L.).]]>357580Lot, H., V. Chovelon, S. Souche and B. Delecolle,1998Onion yellow dwarf and leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars.]]>8213811385Mahmoud, S.Y.M., Abo-El Maaty, A. Sabah, A.M. El- Borollosy and M.H.A. Ghaffar,20072746755Meenakshi, A., V.K. Baranwal, Y.S. Ahlawat and L. Singh,20069112301234Mezei, L.M. and D.R. Storts,19941994pp: 43-57pp: 43-57Pappu, S.S., R. Brand, H.R. Pappu, E.P. Rybicki, K.H. Gough, M.J. Frenkel and C.L. Niblett,199341920Peiwen, X., S. Huisheng, S. Ruijie and Y. Yuanjun,1994358307311Quacquerelli, A., O. Gallitelli, V. Savinow and P. Piazzolla,198015155251Robles, J. and M. Doers,1994®-T vector systems troubleshooting guide.]]>451920Sang-Il, N., P. Ju-Hyun, K. Ki-Seok and U. Jeong-Sik,200229171177Schneider, T.D.,199114883123Shahraeen, N., D.E. Lesemann and T. Ghtbi,200838131135Shukla, D.D. and C.W. Ward,19886927032710Takaichi, M., T. Nagakubo and K. Oeda,2001857175Tsuneyoshi, T., T. Matsumi, K.T. Natsuaki and S. Sumi,199814397113Hernandez, R., H. Bertrand, P. Lepoivre, J.E. Gonzalez and X. Rojas et al.,2002Musa spp. ]]>24247