International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2012.178.190in vitro Cultured Shoot Tip of Banana Cv. Grand Nain for Resistance some Virus Diseases]]>SayedEl- EmanH. MahfouzeSherin A. ShaltoutA.D. El- DougdougKh.A. SayedR.A. 2201282The chemical mutagens have become important tool to enhance agronomic traits of banana crop. It is being used to produce virus resistance in various susceptible banana crop to improve it yield and quality traits against BBTV and BMV viruses. There are several mutagens available for banana crop improvement and each mutagen has its important role as positive or negative effects on banana plants. The chemical mutagens such as create mutations in the genome of plants. Selection of plant mutants is based on morphological and ISSR-PCR markers. The DNA based marker is reliable and reproducible for mutant selection for BBTV and BMV resistance banana plants used in the study. Explants from shoot apical meristem were cultured on MS-medium supplemented with different concentrations of 2, 4-D (2, 4 and 6 mg L-1) 6-Benzylaminopurine (6, 7 and 8 mg L-1) and Sodium Azide ranging from (1, 2 and 3 mg L-1) for induction of mutation. In vitro screened plants after rooting were hardened and acclimatized in the glass house and were shifted into the field. Screening banana plants resistance of BBTV and BMV was carried out by using syringe method of inoculation. It was observed that, only two banana plants of the concentration 6 mg L-1 from 2, 4-D was found to be resistant against BMV isolate. In the same time, it is not BBTV resistance banana plants.]]>Agrawal, G.K., R.N. Pandey and V.P. Agrawal, 1992Choerospondias asillaris leaves.]]>21924Ahloowalia, B.S. and M. Maluszynski,2001118167173Amar, K.R.,20002000Ali, E.M. and S.M. Amer,1974Vicia faba.]]>39633643Bhagwat, B. and E.J. Duncan,1998Musa sp., AAA Group) for tolerance to Fusarium oxysporum f.sp. cubense using chemical mutagens.]]>731122Bhat, T.A., A.H. Khan and S. Parveen,2005Vicia faba L.]]>844548Clark, M.F. and A.N. Adams,197734475483Del Campo, G., R. Antonio and O. Coletto,1999Allium cepa L.]]>7118125Dolezel, J. and F.J. Novak,1984Allium sativum L. meristem root tip cells.]]>26293298El-Den, J.B.,199312810Hussnain, Z. and S. Afghan,2001Colletotrichum falcatum (went) screening.]]>1635Jones, D.A.,20002000pp: 425-434pp: 425-434Koch, W.H., E.N. Henrikson, E. Kupchella and T.A. Cebula,1994Salmonella typhimurium strain TA 100 differentiates several classes of carcinogens and mutagens by base substitution specificity.]]>157988Lakshmanan, V., S.R. Venkataramareddy and B. Neelwarne,200710106113Matsumoto, K., L. Styer Caldas and Y. Yamamoto,2006152729Maniu, M. and A. Mihailescu,1998in vitro mutagenesis in barley. Annals of the University Al I Cuza Siintifice Iasi Section II A.]]>44177182Mendhulkar, V.D.,2002Triticum aestivum Linn.]]>15213219Munthali, T., H.J. Newbury and B.V. Ford-Lloyd,199615474478Murashige, T. and F. Skoog,196215473497Novak, F.J., R. Afza, M.V. Duren and M.S. Omar,1990in vitro cultured shoot-tips of banana and plantain (Musa sp.).]]>672128Pohlheim, E., F. Pohlheim and G. Guenther,197746232232Racharak, P. and W. Eiadthong,2007Musa acuminate colla and A-genome consisting edible cultivated banana assayed with ISSR markers.]]>2914391489Rani, V., A. Parida and S.N. Raina,1995Populus deltoids Marsh.]]>14459462Montalvan, R. and A. Ando, 1998Oryza sativa L.).]]>21244251Roux, N.S.,20042004pp: 23-32pp: 23-32Sandoval, J.A., F.X. Cote and J. Escoute,1996321417Seiler, J.P.,197855197226Shepherd, K. and K.M. Da Silva,19965199103Shirani, S., F. Mahdavi and M. Maziah,2009Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoot tips.]]>857555761Sikka, K. and A.K. Sharma,197642299307Smith, M.K., S.D. Hamill, P.W. Langdon, J.E. Giles, V.J. Doogan and K.G. Pegg,2006Musa spp., AAA group, Cavendish subgroup).]]>46107113Snedecor, G.W. and W.C. Cochran,19726th Edn.,Pages: 593Pages: 593Swennen, R. and D. Vuylsteke,20012001pp: 530-552pp: 530-552Khan, S., F. Al-Qurainy and F. Anwar,20094121Veleminsky, J., J. Satava, A. Kleinhofs, R.A. Nilan and T. Gichner,1985M. luteus endonuclease-sensitive sites in DNA of barley embryos by sodium azide.]]>149431436Vuylsteke, D., R. Swennen and E. de Langhe,1991Musa spp., AAB group) derived from shoot tip culture.]]>46429439Yuan, H.Y. and Z.L. Zhang,1993Hordeum vulgare L.]]>352026Ziv, M.,1991In vitro Plants.]]>1991pp: 45-69pp: 45-69Anders, R.J., K. Bendixen and U. Karlson,2002626832689