International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2009.109.118SidarosS.A. El-KeweyS.A. AminHala. A. KhatabEman. A.H. EmeranA.A. El-KhalikS.A. El-KadyM.A.S. 2200952A Tobamovirus isolated from pepper plants grown in Egypt has been characterized. Pepper mild mottle Tobamovirus (PMMoV) was isolated from naturally infected pepper plants grown in Kafr El-sheikh Governorate. Reverse Transcription Polymerase Chain Reaction (RT-PCR) using a specific non-degenerate primer pair for the PMMoV coat protein gene (PMM-F and PMM-R) revealed 470 bp amplified product. Dot blot hybridization was used to establish the authenticity and specificity to the RT-PCR amplified products of PMMoV. The coat protein gene of an Egyptian isolate of PMMoV was cloned and sequenced. The sequence contained a full-length ORF coding for the viral CP. It comprises 473 nt and a polypeptide chain of 157 amino acids with a M(r) of 17.27. The comparison of the CP ORF nucleotide sequence of Egyptian isolate (PMMoV-E2) with those of another isolates revealed that it was very close to the Brazilian and Japanese isolates (99 and 98%, respectively). Whereas the CP of PMMoV-E2 isolate was closely related to TMV-CP (99 and 97%) of Japan and Korea isolates, respectively. In the meantime it appeared far from Tropical Soda Apple Mosaic Virus-CP (TSAMV-CP) Florida isolate (USA) of Tobomovirus .]]>Alonso, E., I. Garcia Luque, M.J. Avila-Rincon, B. Wicke, M.T. Serra and J.R. Diaz-Ruiz,19891256776Alonso, E., I. García-Luque, A. de la Cruz, B. Wicke and M.J. Avila-Rincón et al.,19917228752884Berzal-Herranz A., A. De La Cruz, F. Tenllado, J.R. Diaz-Ruiz and L. Lopez et al.,1995209498505El-Kady, M.A.S., S.A. Sidaros, S.A. El-Kewey, E.A.H. Khatab, H.A. Amin, A.A. Emeran and S.A. El-Khalik,2007Pepper mild mottle virus affecting pepper plants in Egypt.]]>48192El-Kewey, S.A., M.A.S. El-Kady, S.A. Sidaros, A.A. Emeran and S. Abd El-Khalik,2007Pepper mild mottle virus affecting pepper plants in Egypt.]]>46980Forster, A.C., J. McInnes, D.C. Skingle and R. Symons,198513745761Garcia-Laque, I., M.L. Ferrero, J.M. Rodriguez, E. Alonso and A. De-La Gruz et al.,1993Tobamoviruses in pepper shows that they are different viruses.]]>1317588Hamada, H., S. Takeuchi, A. Kiba, S. Tsuda, Y. Hikichi and T. Okuno,2002Pepper mild mottle virus coat protein that affect L3 gene-mediated resistance in pepper.]]>68155162Hamada, H., Y. Takeuchi, Morita, H. Sawada, A. Kiba and Y. Hikichi,200369199204Jones, R.A.C. and L. Torrance,19861986Kirita, M., K. Akutsu, Y. Watanabe and S. Tsuda,1997Pepper mild mottle Tobamovirus (TMV-P) RNA.]]>63373376Lamb, E.M., S. Adkins, K.D. Shuler and P.D. Roberts,2001Pepper mild mottle virus.]]>2001Leary, J.J., D.J. Brigati and D.C. Ward,19838040454049Letschert, B., G. Adam, D. Lesemann, P. Willingmann and C. Heinze,2002106110Loebenstein, G., F. Akad, V. Filatov, G. Sadvakasova and A. Manadilova,1997Potato leafroll Luteovirus in leaves and tubers with a digoxigenin-labeled cRNA probe.]]>81489491Maniatis, T., E.F. Fritsch and J. Sambrock,19823rd Edn.,Mathews, D.M., K. Riley and J.A. Dodds,1997Citrus tristeza virusin field trees during months on nonoptimal titer.]]>81525529Nagai, Y.,1981Tobacco mosaic virus.]]>91109Nagai, Y., T. Takeuchi and H. Tochihara,198147541546Pereda, S., N. Ehrenfeld, C. Medina, J. Delgado and P. Arce-Johnson,2000Arabidopsis thaliana.]]>90135142Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Shoman, S.A.,2004Tobacco mosaic virus Egyptian strain: Cloning and sequencing of 3 terminal genome sequence-Egypt.]]>18136143Tenllado, F., I. Garcia-Luque, M.T. Serra and J.R. Diaz-Ruiz,1994Tobomoviruses infecting L-resistant genotypes of pepper by RT-PCR and restriction analysis.]]>47165174Torok, V.A. and J.W. Randles,2001Tobacco mosaic virus RNA as an internal control for duplex RT-PCR assay of pea germplasm.]]>30227230Tsuda, S., M. Kirita and Y. Watanabe,1998Pepper mild mottle Tobamovirus strain capable of overcoming the L3 genemediated resistance, distinct from the resistance-breaking Italian isolate.]]>11327331Velasco, L., D. Janssen, L. Ruiz-Garcia, E. Segundo, I.M. Cuadrado,2002Pepper mild mottle virus (PMMoV) isolate that overcomes L3 resistance in pepper.]]>106135140Wang, X., F. Liu, G. Zhou, X.H. Li and Z. Li,2006Pepper mild mottle virus in China.]]>154755757Wetter, C., M. Conti, D. Altschuh, R. Tabillion and M.H.V. Van Regenmortel,1984Pepper mild mottle virus, a tobamovirus infecting pepper cultivars in Sicily.]]>74405410Wetter, C. and M. Conti,1988Pepper mild mottle virus.]]>1988Wilcockson, J. and R. Hull,197423107111