International Journal of Virology1816-4900xxxx-xxxxAcademic Journals Inc.10.3923/ijv.2008.14.25SolimanA.M. BarsoumB.N. MohamedG.G. RezkA.A. Aboul-AtaA.E. MazyadH.M. 1200841This study, aims at determination of efficiency of micro interfering
RNA (miRNA) to develop ability of virus resistance against Egyptian PVX
isolate (PVX-Eg2) in both potato (Solanum tuberosum L. cv. Spunta)
and tobacco (Nicotiana benthamiana). RNA constructs of Sense (PVX-Eg2cpVs),
antisense (PVX-Eg2cpCs) and sense/antisense were designed, cloned and
sub- cloned for gene transfection using Agrobacterium inoculation
technique. Two to 3 leaf-stage seedlings of potato (Solanum tuberosum
L. cv. Spunta) and tobacco (Nicotiana benthamiana) were
inoculated with the three previous constructs. The construct-treated plants
were mechanically inoculated with the PVX-Eg2 isolate. Bioassay and PCR
amplification have been able to evaluate transfected-plant resistance
against PVX-Eg2 that is caused by siRNA of PVX-Eg2cp. PCR amplification
has been able to detect PVX viral genome in all challenged plants those
infiltrated with either pFGC5491 vector without insert, or with sense
construct and also with antisense construct. Bioassay has confirmed same
previous statement. Nine out of 10 sense/antisense-transfected potato
plants were negatively reacted with both bioassay and PCR amplification.
Same negative reaction has been viewed using both bioassay and PCR for
sense/antisense transfected-tobacco plants. Seven out of 10 proved they
are PVX-Eg2 resistant.]]>Abdel-Salam, A.M., A.M.A. Ashoub, T.M. Nasr El-Din and I.A.M. Ibrahim,199422221239Angell, S.M. and D.C. Baulcombe,19971636753684Bass, B.L.,2000101235238Beachy, R.N.,19978215220Eckardt, N.A.,20021414331436El-Amrety, A.A.,19701970Fahmy, F.G. and M.S. Mohamed,1984166569Feng, D., X. Liu, K. Meng, L. Liao, X. Wei, H. Xu and Z. Zhu,20034815921598Hamilton, A. and D.C. Baulcombe,1999286950952Hammond, S.M., A.A. Caudy and G.J. Hannon,20012110119Haywood, V., F. Kragler and W.J. Lucas,200214303325Horton, D.,1992Johansen, L.K. and J.C. Carrington,2001Agrobacterium-mediated transient expression system.]]>126930938Llave, C., K.D. Kasschau, M.A. Rector and J.C. Carrington,20021416051619Lucas, W.J., B.C. Yoo and F. Kragler,20012849857Orman, B.E., R.M. Celnik, A.M. Mandel, H.N. Torres and A.N. Mentaberry,199016293306Ratcliff, F.G., S.A. McFarlane and D.C. Baulcombe,19991112071215Ruiz-Medrano, R., B. Xoconostle-Cazares and W.J. Lucas,20014202209Salazar, L.F.,1996Pages: 214Pages: 214Shalaby, A.A., M.K. Nakhla, A.M. Soliman, H.M. Mazyad, A. Hadidi and D.P. Maxwell,20025275286Smith, K.M.,1931109251267Soliman, A.M., A.A. Shalaby, B.N. Barsoum, G.G. Mohamed, M.K. Nakhla, H.M. Mazyad and D.P. Maxwell,2000Potato virus X (PVX) isolate from Egypt.]]>917911804Soliman, A.M., B.N. Barsoum, G.G. Mohamed, A.K. El-Attar and H.M. Mazyad,2006Escherichia coli and production of polyclonal antibodies against it.]]>9115128Vance, V. and H. Vaucheret,200129222772280Voinnet, O.,200117449459Voinnet, O.,20025444451Waterhouse, P.M., M.B. Wang and T. Lough,2001411834842Wesley, S.V., C.A. Helliwell, N.A. Smith, M. Wang, D.T. Rouse, Q. Liu and P.S. Gooding,200127581590Yousef, A.A.,19921992