International Journal of Poultry Science1682-83561994-7992Asian Network for Scientific Information10.3923/ijps.2019.168.173LokaewmaneeKanda 42019184Objective: This study was conducted to determine the effect of chili leaf on laying hen performance, egg quality and egg yolk cholesterol levels. Materials and Methods: The completely randomized design involved evaluation of a control (no chili leaf powder) diet and experimental diets supplemented with chili leaf powder (CLP) at 1, 2 or 3%. One hundred and twenty laying hens (Charoen Pokphand Brown) at 61 weeks of age were divided into 4 treatments, each with 10 replicates (3 birds per replicate). Each group was randomly allocated to one of the treatments for 5 weeks. All diets were isonitrogenous and isocaloric. Results: The results of the effect of supplementation with CLP at 0, 1, 2 and 3% showed that the body weight gain, egg yield, egg weight and cholesterol were not significantly different among the treatments (p>0.05). However, supplementation with CLP at 0 and 1% resulted in a higher feed intake and feed conversion ratio than those obtain with CLP supplementation at 3% (p<0.05). There were no significant differences among treatments in egg quality, breaking strength, shell thickness, yolk color, yolk percentage, shell percentage, albumen percentage, Haugh unit, lightness (L*) of yolk color, redness of yolk color (a*) and yellowness (b*) of yolk color (p>0.05). Conclusion: Dietary supplementation with 3% CLP can be used as alternative feed additive for laying hens despite an adverse effect on feed intake and feed conversion ratio, as feeding 3% CLP had no adverse effects on egg quality.]]>Sim, J.S. and H.H. Sunwoo,20082008pp: 19-36pp: 19-36Hashemi, S.R. and H. Davoodi,201135169180Lokaewmanee, K., S. Mompanuon, P. Khumpeerawat and K. Yamauchi,2009Morus alba L.) on egg yolk color.]]>46112115An, B.K., H.S. Kwon, B.K. Lee, J.Y. Kim, S.J. You, J.M. Kim and C.W. Kang,2010Scutellaria baicalensis) extract on laying performance and lipid oxidation of chicken eggs.]]>23772776Ghasemi, R., M. Zarei and M. Torki,2010Allium sativum) and thyme (Thymus vulgaris) to diet of laying hens and evaluating productive performance and egg quality characteristics.]]>5151154Lokaewmanee, K., K. Yamauchi, T. Komori and K. Saito,2011112571260Hu, C.H., A.Y. Zuo, D.G. Wang, H.Y. Pan, W.B. Zheng, Z.C. Qian and X.T. Zou,2011170117121Duru, M.,2013Fragaria ananassa Duch.) leaf powder on egg yield, quality and egg yolk cholesterol in laying hens.]]>11477480Boka, J., A.H. Mahdavi, A.H. Samie and R. Jahanian,2014Nigella sativa L.) on performance, intestinal Escherichia coli colonization and jejunal morphology in laying hens.]]>98373383Cayan, H. and G. Erener,2015Olea europaea) powder on laying hens performance, egg quality and egg yolk cholesterol levels.]]>28538543Lokaewmanee, K. and P. Promdee,201817221228Holzer, P.,1990298316Daood, H.G., M. Vinkler, F. Markus, E.A. Hebshi and P.A. Biacs,199655365372Krinsky, N.I.,200117815817AOAC.,200518th Edn.,NRC.,19949th Rev. Edn.,Pages: 176Pages: 176Stadelman, W.J.,19951995pp: 39-66pp: 39-66CIE.,19761976Tilki, M. and M. Saatci,2004Alectoris graeca) eggs.]]>155561564Dresselhaus, M. and L. Acker,197428137142SAS Institute,1996Steel, R.G.D. and J.H. Torrie,19802nd Edn.,El Bagir, N.M., A.Y. Hama, R.M. Hamed, A.G. Abd El Rahim and A.C. Beynen,2006Nigella sativa).]]>5574578Akhtar, M.S., Z. Nasir and A.R. Abid, 2003Nigella sativa L. seeds on poultry egg production and their suitability for human consumption.]]>73181190Voemesse, K., A. Teteh, D. Nideou, O. N'nanle, M. Gbeassor, E. Decuypere and K. Tona,2018Moringa oleifera leaf meal on growth performance and blood parameters of egg type chicken during juvenile growth.]]>17154159Aydin, R., M. Karaman, T. Cicek and H. Yardibi,2008Nigella sativa L.) supplementation into the diet of the laying hen positively influences egg yield parameters, shell quality and decreases egg cholesterol.]]>8725902595USDA.,1991Hargis, P.S.,1988441729Bok, S.H., Y.W. Shin, K.H. Bae, T.S. Jeong, Y.K. Kwon, Y.B. Park and M.S. Choi,20002010071015Kim, H.J., G.T. Oh, Y.B. Park, M.K. Lee, H.J. Seo and M.S. Choi,20047416211634Elkin, R.G.,200662665687Lu, W., J. Wang, H.J. Zhang, S.G. Wu and G.H. Qi,2016Moringa oleifera leaf in laying hens: Effects on laying performance, egg quality, plasma biochemistry and organ histopathological indices.]]>15658665