International Journal of Poultry Science1682-83561994-7992Asian Network for Scientific Information10.3923/ijps.2017.37.42Campylobacter
Colonization in Broiler Chickens]]>ShresthaS. ArsiK. WagleB.R. DonoghueA.M. DonoghueD.J. 22017162Background and Objective:Campylobacter is the leading cause of foodborne enteritis worldwide and is primarily caused by consumption/mishandling of contaminated poultry. Probiotic use in poultry has been an effective strategy in reducing many enteric pathogens, but has not demonstrated consistent reduction against Campylobacter. This study was conducted to screen probiotic isolates that could eliminate or reduce cecal Campylobacter counts in poultry. Materials and Methods: As Campylobacter resides and utilizes intestinal mucin for growth, isolates selected on the basis of mucin utilization might be a strategy to screen for probiotic candidates with efficacy against Campylobacter . In this study, bacterial isolates demonstrating increased growth rates in the presence of mucin in media, in vitro were selected for their ability to reduce Campylobacter colonization in 14 day old broiler chickens. In replicate trials, 90 days-of-hatch chicks were randomly divided into 9 treatment groups (n = 10 chicks/treatment) and treated individually with one of four bacterial isolates (Bacillus spp.) grown in media with or without mucin prior to inoculation or a Campylobacter control (Campylobacter , no isolate). In both the trials, all the birds except control were orally gavaged with individual isolates at day-of-hatch. On day 7, all the birds were orally challenged with a four strain mixture of C. jejuni and ceca were collected on day 14 for Campylobacter enumeration. Results: Results from these two trials demonstrated two individual isolates, one isolate incubated with mucin in the media and another isolate incubated without mucin prior to inoculation, consistently reduced cecal Campylobacter counts (1.5-4 log reduction) compared to controls. Conclusion: These results support the potential use of mucin to pre-select isolates for their ability to reduce enteric colonization of Campylobacter in broiler chickens.]]>WHO.,2011Campylobacter.]]>2011CDC.,20152015CDC.,2013Campylobacter: General information.]]>2013Lastovica, A.J.,2006Campylobacter spp.: The tip of the iceberg.]]>284956Debruyne, L., D. Gevers and P. Vandamme,20082008pp: 3-26pp: 3-26Friedman, C.R., J. Neimann, H.C. Wegener and R.V. Tauxe,2000Campylobacter jejuni Infections in the United States and Other Industrialized Nations.]]>2000pp: 121-138pp: 121-138Park, S.F.,2002Campylobacter species and its relevance to their role as foodborne pathogens.]]>74177188Snelling, W.J., M. Matsuda, J.E. Moore and J.S.G. Dooley,2005Campylobacter jejuni.]]>41297302Blaser, M.J. and J. Engberg,2008Campylobacter jejuni and Campylobacter coli infections.]]>2008pp: 97-121pp: 97-121King, E.O.,1962Vibrio fetus and a closely related Vibrio isolated from cases of human vibriosis.]]>98700711Skirrow, M.B.,1977Campylobacter enteritis: A new disease.]]>2911Rosenquist, H., N.L. Nielsen, H.M. Sommer, B. Norrung and B.B. Christensen,2003Campylobacter species in chickens.]]>8387103Stern, N.J., P. Fedorka-Cray, J.S. Bailey, N.A. Cox and S.E. Craven et al.,2001Campylobacter spp. in selected U.S. poultry production and processing operations.]]>6417051710Friedman, C.R., R.M. Hoekstra, M. Samuel, R. Marcus and J. Bender et al.,2004Campylobacter infection in the United States: A case-control study in foodnet sites.]]>38S285S296Mylius, S.D., M.J. Nauta and A.H. Havelaar,2007Campylobacter.]]>27803813Loc Carrillo, C., R. Atterbury, A. El-Shibiny, P. Connerton, E. Dillon, A. Scott and I.F. Connerton,2005Campylobacter jejuni colonization of broiler chickens.]]>7165546563Stern, N.J., E.A. Svetoch, B.V. Eruslanov, V.V. Perelygin and E.V. Mitsevich et al.,2006Lactobacillus salivarius strain and purification of its bacteriocin, which is inhibitory to Campylobacter jejuni in the chicken gastrointestinal system.]]>5031113116De los Santos, F.S., A.M. Donoghue, K. Venkitanarayanan, M.L. Dirain, I. Reyes-Herrera, P.J. Blore and D.J. Donoghue,2008Campylobacter jejuni colonization in ten-day-old broiler chickens.]]>87800804De los Santos, F.S., A.M. Donoghue, K. Venkitanarayanan, I. Reyes-Herrera and J.H. Metcalf et al.,2008Campylobacter jejuni colonization in broiler chicks.]]>7445644566Metcalf, J.H., A.M. Donoghue, K. Venkitanarayanan, I. Reyes-Herrera, V.F. Aguiar, P.J. Blore and D.J. Donoghue,2011Campylobacter colonization in broilers.]]>90494497Arsi, K., A.M. Donoghue, A. Woo-Ming, P.J. Blore and D.J. Donoghue,2015Campylobacter colonization in broiler chickens.]]>24327334Arsi, K., A.M. Donoghue, A. Woo-Ming, P.J. Blore and D.J. Donoghue,2015Campylobacter colonization in broiler chickens.]]>78209213Hermans, D., K. van Deun, W. Messens, A. Martel and F. van Immerseel et al.,2011Campylobacter control in poultry by current intervention measures ineffective: Urgent need for intensified fundamental research.]]>152219228Fuller, R.,198966365378Soerjadi, A.S., S.M. Stehman, G.H. Snoeyenbos, O.M. Weinack and C.F. Smyser,1981Salmonella and Escherichia coli by competitive exclusion in chickens.]]>25706712Impey, C.S., G.C. Mead and S.M. George,1982Salmonellas from the chick caecum using a defined mixture of bacterial isolates from the caecal microflora of an adult bird.]]>89479490Hakkinen, M. and C. Schneitz,1996Escherichia coli and E. coli O157:H7.]]>139139141Hume, M.E., J.A. Byrd, L.H. Stanker and R.L. Ziprin,1998Listeria monocytogenes in leghorn chicks following treatment with a competitive exclusion culture (PREEMPT™).]]>26432436Hume, M.E., D.E. Corrier, D.J. Nisbet and J.R. Deloach,1998Salmonella challenge time and reduction in chick cecal colonization following treatment with a characterized competitive exclusion culture.]]>61673676Upadhyay, A., I. Upadhyaya, S. Mooyottu and K. Venkitanarayanan,2016Listeria monocytogenes virulence in vitro and in invertebrate model Galleria mellonella.]]>65443455Stern, N.J., N.A. Cox, J.S. Bailey, M.E. Berrang and M.T. Musgrove,2001Salmonella and Campylobacter spp. colonization in broiler chickens.]]>80156160Robyn, J., G. Rasschaert, D. Hermans, F. Pasmans and M. Heyndrickx,2013In vivo broiler experiments to assess anti-Campylobacter jejuni activity of a live Enterococcus faecalis strain.]]>92265271Jonsson, H., E. Strom and S. Roos,2001Lactobacillus reuteri in vitro.]]>2041922Lee, A., J.L. O'rourke and P.J. Barrington,1986Campylobacter jejuni: A mouse cecal model.]]>51536546Beery, J.T., M.B. Hugdahl and M.P. Doyle,1988Campylobacter jejuni.]]>5423652370Hugdahl, M.B., J.T. Beery and M.P. Doyle,1988Campylobacter jejuni.]]>5615601566Farnell, M.B., A.M. Donoghue, K. Cole, I. Reyes-Herrera, P.J. Blore and D.J. Donoghue,2005Campylobacter susceptibility to ciprofloxacin and corresponding fluoroquinolone concentrations within the gastrointestinal tracts of chickens.]]>9910431050Line, J.E.,2001Campylobacter spp.]]>6417111715Byrd, J.A., R.C. Anderson, T.R. Callaway, R.W. Moore and K.D. Knape et al.,2003Salmonella typhimurium contamination of broilers.]]>8214031406SAS.,2011® 9.3 Procedures Guide: Statistical Procedures.]]>Aguiar, V.F., A.M. Donoghue, K. Arsi, I. Reyes-Herrera, and J.H. Metcalf et al.,2013Campylobacter jejuni in broiler chickens.]]>10435441Naughton, J., G. Duggan, B. Bourke and M. Clyne,201454852Zhu, X.Y., T. Zhong, Y. Pandya and R.D. Joerger,200268124137Berg, R.D.,19964430435Zarate, G., V.I.M. de Ambrosini, A.P. Chaia and S.N. Gonzalez,2012in vitro and in vivo.]]>65534539Ouwehand, A.C., P.V. Kirjavainen, M.M. Gronlund, E. Isolauri and S.J. Salminen,19999623630