International Journal of Poultry Science1682-83561994-7992Asian Network for Scientific Information10.3923/ijps.2014.253.259Campylobacter jejuni in Poultry Products Using Quantum Dots and Nanobeads Based Fluorescent Immunoassay]]>WangHong LiYanbin SlavikMichael 52014135Campylobacter jejuni causes 2.1 to 2.4 million cases of
foodborne illnesses in the United States each year with some of the cases linked
to eating undercooked poultry or handling raw poultry and poultry products.
Thus, a rapid, specific method is needed to detect C. jejuni on poultry
and poultry products. The objective of this research was to develop a sensitive
immunoassay method for rapid detection of C. jejuni by using both magnetic
nanobeads to separate and concentrate the target bacteria and quantum dots (QDs)
as fluorescent markers. In this research, both streptavidin conjugated QDs 620
(8 nm diameter) and magnetic nanobeads (150 nm diameter) were separately coated
with the specific biotin conjugated anti-C. jejuni antibody. The conjugated
magnetic nanobeads then were mixed with a sample containing C. jejuni.
After immunomagnetic separation, the magnetic nanobeads-C. jejuni conjugates
were mixed with the conjugated QDs. Then, unattached conjugated QDs were removed
using immunomagnetic separation. A spectrometer was used to measure the fluorescence
of the complexes of magnetic beads-C. jejuni-QDs. The results showed
that this method could detect C. jejuni in pure culture, ground turkey,
chicken juice or chicken carcass wash solution at concentrations down to 2-3
cells/0.1 mL sample (20-30 cfu/mL). The total detection time was less than 2
h. This study would provide the poultry industry a more effective rapid method
for detection of major foodborne pathogens in poultry products to ensure food
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