International Journal of Poultry Science
1682-8356
1994-7992
Asian Network for Scientific Information
10.3923/ijps.2005.741.744
Musa
H. H.
Li
B. C.
Chen
G. H.
Lanyasunya
T. P.
Q. Xu
Bao
W. B.
10
2005
4
10
Traditional karyotyping
is invented in animal research for several decades depend on the analysis
of characteristic banding patterns along the length of chromosome. In
the present study chicken metaphase chromosomes were obtained by peripheral
blood lymphocyte culture techniques, G-band patterns were obtained with
trypsin and Giemsa, C-band patterns were treated with barium and the
nuclear organizer regions (NORs) were identified by silver staining.
All species studied presented a diploid number of 78 chromosomes, with
10 pairs of macro chromosomes including the sex chromosome and 29 pairs
of micro chromosomes. G-band patterns were found quite different between
breeds. The dark stained of C-band was observed on micro chromosome
and W chromosome. Karyotype resemblance near coefficient was possible
for breeds clustering. The position of centromers, relative length,
arm ratio and the evolutionary distance of chicken breeds was estimated.
The application of chromosome karyotype and banding techniques was used
to study the origin, evolution and relationship of species, also used
for gene location and sex determination. While, in the Medical field
was used to identify genetic disease. The techniques was consider as
a base for further molecular research, for example FISH.]]>