International Journal of Plant Breeding and Genetics1819-35952152-3347Academic Journals Inc.10.3923/ijpbg.2015.238.246Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)]]>IslamShahidul MirJaveed Iqbal KudesiaRajdeep 4201594Seed storage protein profiling through SDS-PAGE and DNA Restriction Fragment Length Polymorphism (RFLPs) were surveyed in 5 accessions of mungbean (Vigna radiata L.) sampled from NBPGR Thrissur, Kerala, India, to evaluate genetic diversity. The protein profile showed a low level of polymorphism (26.66%). The similarity index calculated ranged from (42.30-50%). The dendrogram constructed revealed two clusters. Cluster 1st comprises two accessions IC 251432 and IC 251433. Cluster 2nd comprises 3 cultivars viz., IC 251431, IC 251430 and IC 251434. Cluster 2nd also revealed a 100% homology between IC 251431 and IC 251430 accessions. The RFLP analysis was carried out by single restriction enzyme EcoRI. The RFLP profile revealed a high level of polymorphism (90.90%). A total of 11 clear bands were scored. The only band with fragment size 630 kb was monomorphic while, the rest were polymorphic. Similarity index ranged from (42.82-11.11%). The UPGMA analysis divided the accessions into 2 clusters viz., cluster 1st comprising 4 accessions (IC 251431, IC 251432, IC 251433 and IC 251430). Cluster 2nd includes only one accession (IC 251434) occupying a distinct position in constructed dendrogram.]]>Arumuganathan, K. and E.D. Earle,19919208218Chowdhury, M.A., B. Vanderberg and T. Warkentin,2002Cicer arietinum L.).]]>127317325Cooke, R.J.,198455972Diers, B.W., P. Keim, W.R. Fehr and R.C. Shoemaker,199283608612Doyle, J.J.,1990121315Faris, D.G.,1965Vigna sinensis.]]>7433452Gardiner, S.E. and M.B. Forde,198811326Gentzbittel, L., A. Perrault and P. Nicolas,1992Helianthus genus, based on nuclear restriction fragment length polymorphism (RFLP).]]>9872892Ghafoor, A., Z. Ahmad and M. Afzal,2005Vignamungo (L.) Hepper] Germplasm.]]>37263269Ghallab, K.H., A.M. Ekram, S.A. Afiah and S.M. Ahmed,200757112Hameed, A., M. Qureshi, M. Nawaz and N. Iqbal,20124419931999Jha, S.S. and D. Ohri,1996Cajanus cajan (L.) Millsp. (pigeonpea) and its wild relatives based on seed protein profiles.]]>43275281Kaga, A., N. Tomooka, Y. Egawa, K. Hosaka and O. Kamijima,1996Genus vigna) as revealed by RAPD analysis.]]>881724Keim, P., B.W. Dier, T.C. Olson and R.C. Shoemaker,1990126735742Lambrides, C.J. and I. Godwin,20072007pp: 69-90pp: 69-90Lee, M., E.B. Godshalk, K.R. Lamkey and W.W. Woodman,19892910671071Manella, G., S.V. Onofaro, A. Tonidni and V. Mangifico,1999Solanum species and of cultivars and androgenic lines of S. melongena L. by SDS-PAGE.]]>272335 Nodari, R.O., E.M.K. Koinange, J.D. Kelly and P. Gepts,199284186192Rabbani, M.A., M.A. Qureshi, R. Anwar and S. Komatsu,2001Brassica juncea (L.) czern. & coss] germplasm by SDS-PAGE of total seed proteins.]]>33173179Sadia, M., S.A. Malik, M.A. Rabbani and S.R. Pearce,2009Brassica species.]]>514Shafique, S., M.R. Khan, M. Nisar and S. Rehman,2011Vignamungo (L.) Hepper].]]>4312231232Silberstein, L., I. Kovalski, R. Huang, K. Kostas-Anagnostou, M.M.K. Jahn and R. Perl-Treves,1999Cucumis melo L.) as revealed by RFLP and RAPD markers.]]>79101111Nair, R.M., R. Schafleitner, L. Kenyon, R. Srinivasan, W. Easdown, A. Ebert and P. Hanson,20122012pp: 2728Singh, D.P. and I.P.S. Ahlawat,2005Vigna radiata) and Black gram (Vigna mungo) improvement in India: Past, present and future prosdpects.]]>75243250Singh, R., S.G. Tan, J.M. Panandam, R.A. Rahman and S.C. Cheah,2008Elaeis guineensis).]]>165363Smith, O.S., J.S.C. Smith, S.L. Bown, R.A. Tenborg and S.J. Wall,19901 grain yield, grain yield, heterosis and RFLPs.]]>80833840Beckmann, J.S. and M. Soller,198635111124Song, K., T.C. Osborn and P.H. Williams,199079497506Vavilov, N.I.,19261926Velasquez, V.L.B. and P. Gepts,1994Phaseolus vulgaris) in its centres of origin.]]>37256263Win, K.T., Z.O. Aung, T. Hirasawa, T. Ookawa and H. Yutaka,2011Myanmar vigna species in responses to salt stress at the seedling stage.]]>1016151624Zehr, B.E., J.W. Dudley, J. Chojecki, M.A.S. Maroof and R.P. Mowers,199283903911Zhang, Q., M.A. Maroof and A. Kleinhofs,1993Hordeum vulgare ssp. spontaneum.]]>134909916Srivastava, R.P. and M. Ali,20042004Soller, M. and J.S. Beckmann,1983672533