International Journal of Plant Breeding and Genetics1819-35952152-3347Academic Journals Inc.10.3923/ijpbg.2014.121.138Ipomoea batatas (L.)) Accessions in Ghana via, Meristem and Nodal Culture]]>Addae-FrimpomaahF. AmponsahJ. TengeyT.K. 3201483In vitro regeneration of three sweet potato accessions UE007, UK-BNARI
and SA-BNARI using meristem or nodal cuttings was studied. Meristematic explants
cultured on (Murashige and Skoog, 1962) basal medium supplemented with low concentration
of benzylaminopurine (BAP) or kinetin resulted in callus with or without shoot
development which delayed shoot emergence. The degree of callus development
increased as the concentration of the cytokinin in the culture medium increased.
Although, callus development was comparatively lower on kinetin amended medium
than BAP amended medium (Murashige and Skoog, 1962) medium supplemented with
0.25 mg L-1 BAP had the highest shoot induction (80%). For further
differentiation of callus or shoots into distinct stem and leaves, the cultures
were transferred into fresh MS medium supplemented with 0.25 mg L-1
BAP, 0.1 mg L-1 NAA and 0.1 mg L-1 gibberellic acid. To
overcome the delay in shoot initiation using meristem culture, nodal cuttings
of sweet potato were used as explants and cultured on MS medium amended with
0.3-0.9 mg L-1 BAP. All explants cultured on 0.3 or 0.6 mg L-1
BAP developed shoots. Post flask acclimatisation of meristem or nodal cutting
derived plantlets showed that meristem derived plantlets were better acclimatised
than nodal cutting plants due to vigorous root development leading to higher
percentage survival in pots and subsequent tuber production as well as other
yield parameters. The successful regeneration of sweet potato plantlets in
vitro using meristem and nodal cutting explants could be used to complement
conventional propagation methods and integration into plant breeding programmes
for sweet potato.]]>Alam, I., S.A. Sharmin, M.K. Naher, M.J. Alam, M. Anisuzzaman and M.F. Alam,2010Ipomoea batatas (L.) Lam.).]]>33539Badoni, A. and J.S. Chauhan,2010116Barka, G.D.,2009In vitro production of virus free sweet potato [Ipomoea batatas (L.) Lam] by Meristem culture and thermotherapy.]]>2009Bourke, R.M.,1985Ipomoea batatas) production and research in Papua new Guinea.]]>3389108Brabet, C., D. Reynoso, D. Dufour, C. Mestres, J. Arredondo and G. Scott,19981998Carey, E.E. and D. Reynoso,19991999pp: 170-186pp: 170-186Chalageri, G. and U.V. Babu,2012In vitro plant regeneration via petiole callus of Viola patrinii and genetic fidelity assessment using RAPD markers.]]>36358368CIP,2010in vitro gene bank in the world.]]>2010CIP,19911991pp: 6-48pp: 6-48El Far, M.M., K. El Mangoury and H.E.M. Elazab,2009Ipomoea batatas (L.) Lam.) cv. abeesvia indirect organogenesis stimulated by initiation medium and cytokinin effects.]]>3543551FAO,2010FAO,2008George, E.F., J. Van-Staden and P. Zazimalova,20082008pp: 200-218pp: 200-218Hahn, S.K.,19771977pp: 237-248pp: 237-248Isogai, S., K. Touno and K. Shimomura,2008Cephaelis ipecacuanha.]]>44216220Kaeppler, S.M., H.F. Kaeppler and Y. Rhee,200043179188Kapinga, R., O. Oscar, J. Ndunguru, E. Omiat and S. Tumwegamire,2007pp: 5-7pp: 5-7Karthikeyan, K., C. Chandran and S. Kulothungan,2009in vitro axillary shoot proliferation of Centella asiatica L.]]>8232235Larkin, P.J. and W.R. Scowcroft,198160197214Li, L. and C.H. Kao,1985Ipomoea batatas L. Lam. cultivars.]]>1311023Lin, K.H., Y.C. Lai, K.Y. Chang, Y.F. Chen, S.Y. Hwang and H.F. Lo,200748283292Liu, Y., Q.F. Cai, H.M. Song, Z.S. An and H.W. Linderholm,20115629862994Murashige, T. and F. Skoog,196215473497Nagib, A., S.A. Hossain, M.F. Alam, M.M. Hossain, R. Islam and R.S. Sultana,20032616622Oggema, J.N., M.G. Kinyua, J.P. Ouma and J.O. Owuoche,2007Ipomoea batatas (L.) Lam.) cultivars derived from tissue culture regenerated plants.]]>614181425Ozturk, M., K.M. Khawar, H.H. Atar, C. Sancak and S. Ozcan,2004In vitro micropropagation of the aquarium plant Ludwigia repens.]]>122125Panta, A., C. Ynouye, C. Espinoza and W. Roca,20062006Pop, T.I., D. Pamfil and C. Bellini,201139307316Prakash, S. and K. Hinata,1980Pages: 57Pages: 57Pruski, K.,20012001Purseglove, J.W.,1991pp: 50-56pp: 50-56Sexton, R. and J.A. Roberts,198233133162Vengadesan, G., A. Ganapathi, S. Amutha and N. Selvaraj,2002In vitro propagation of Acacia species-a review.]]>163663671Wondimu, T.,2009Ipomoea batatas (L.) Lam) varieties for the production of virus free planting material.]]>2009Woolfe, J.A.,1992Pages: 643Pages: 643Yang, X.,2010in vitro of purple-coloured sweet potato (Ipomoea batatas (L.) Lam.).]]>4220692075GenStat,20079th Edn.,