International Journal of Plant Breeding and Genetics1819-35952152-3347Academic Journals Inc.10.3923/ijpbg.2011.255.267LongXiang-Yu LiuYa-Xi RocheleauHelene OuelletTherese ChenGuo-Yue 3201153Using Quantitative real-time PCR to quantify gene expression, it is vital that one or more ideal internal control genes are used to normalize the samples to be compared. 10 traditional internal control genes were selected to evaluate the stability of expression in wheat leaves infected by strip rust, by qRT-PCR, including 18S Ribosomal RNA (18S), 26S Ribosomal RNA (26S), 60S Ribosomal protein L18a-1(60S), Actin, Cyclophilin (Cyp), Elongation factor 1 alpha (EF1A), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Histone H3 (Histone),α-Tubulin and β-Tubulin. Expression stability of internal control genes was statistically analyzed by three different mathematical algorithms, GeNorm, NormFinder and BestKeeper. 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