International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2022.1440.1448Patrinia villosa Extract and Analysis of its Components based on UPLC-QTOF-MS/MS]]>YangXin XinWangShuai ZhouLi PingLiTian JiaoZhaoLin MengXian ShengGaoFeng BaoYong Rui72022187Background and Objective:Patrinia villosa was first recorded in Shennong Bencao Jing and enjoys a long medicinal history. Modern pharmacological studies show that it has definite anti-tumour pharmacological activity. The objective of this study was to determine its pharmacological activity against colon cancer metastasis and reveal its active components. Materials and Methods: In this study, the CCK-8 method, Transwell chamber experiment and wound healing assay were adopted to explore the inhibitory activity of Patrinia villosa extract on the proliferation, migration and invasion of human colon cancer SW620 cells. Furthermore, UPLC-QTOF-MS/MS technique was used to analyze the extract and the drug-containing plasma after oral administration of the extract in mice. Results:Patrinia villosa extract can inhibit the proliferation, migration and invasion of human colon cancer SW620 cells. Eighteen chemical components, including salicylic acid, daphnetin, oleuropein, isochlorogenic acid B and luteolin were identified in the extract. Among them, daphnetin, oleuropein and isochlorogenic acid B were first discovered in Patrinia. Besides, six chemical components, including salicylic acid and luteolin were introduced into the blood with prototypes. Conclusion: This study provides a basis for the clinical rational application of Patrinia villosa in the treatment of colon cancer metastasis and the disclosure of its pharmacodynamic material basis.]]>Liu, Y.C., N. Sui, J.Q. Wang, X. Wang, W. Liu, Z. Xiang and C.L. Chen,2021Patrinia villosa (Thunb.) Juss.]]>2021Liu, Y., W. Liu, C. Chen, Z. Xiang and H. Liu,2019Patrinia villosa Juss.]]>1913991404Jia, Y.X., L. Wei, Z. Ying, C. Ning and X. Ying et al.,2016Patrinia villosa (Thunb.) Juss.]]>1213He, X., F. Luan, Z. Zhao, N. Ning and M. Li et al.,201745637666Zhang, T., Q. Li, K. Li, Y. Li, J. Li et al.,2008Patrinia villosa (Thunb.) Juss on mice bearing U14 cervical cancer.]]>22640645Song, S.S., Y.R. Bao, H.J. Zhao, Z. Xiang and X.X. Meng et al.,201816161166Xia, L., B. Zhang, Q. Yan and S. Ruan,201850321522159Han, X., S. Wang, X. Yang, T. Li and H. Zhao et al.,2019Patrinia villosa (Thunb.) Juss. effective parts by UPLC-Q-TOF-MS.]]>2019Li, C., W. Fang, Q. Wu, Z. Yao and J. Wu et al.,2021104983998Zheng, Z., S. Li, Y. Zhong, R. Zhan, Y. Yan, H. Pan and P Yan,2017Rubia cordifolia L. extract.]]>2017Ren, Q., S. Zhao, C. Ren and Z. Ma,20182018Yang, H., H. Zhang, Y. Yang, X. Wang and T. Deng et al.,20201082118226Hu, C., M. Li, T. Guo, S. Wang and W. Huang et al.,20192019Huyrigqi, C. Sally, L. Longfei, F. Jing and D. Xiaoxu et al.,20174219641970Zhou, Z.M., D.M. Yan, Y.K. Wang, T. Zhang and X.R. Xiao et al.,2021Rubus Chingii Hu using UPLC-ESI-QTOF-MS.]]>2021Chen, Q.Q., H.L. XI, C.Y. Wang, F.G. Xu and W. Zhang,201715234240Xue, X., X. Si-Min, G. Ying-Min, L. Shan-Shan, W. Chun-Rong and Y. Shi-Kai,201935253260Farag, M.A., H. Handoussa, M.I. Fekry and L.A. Wessjohann,2016710771086Dan-Dan, L., P. Hua-Sheng, Z. Yuan, H. Xian-Zhang and Z. Zhi-Lai et al.,2020Artemisia stolonifera and Artemisia argyi using UPLC-Q-TOF-MS.]]>4540574064Happi, G.M., G.P.M. Kemayou, H.G. Stammler, B. Neumann and M. Ismail et al.,2021Entandrophragma candollei (Meliaceae).]]>2021Wu, Z., M. Jia, W. Zhao, X. Huang and X. Yang et al.,20222022Wang, L., X. Pu, X. Nie, D. Wang and H. Jiang et al.,20212021Nounu, A., R.C. Richmond, I.D. Stewart, P. Surendran and N.J. Wareham et al.,20212021Miao, M. and L. Xiang,20202020pp: 71-88pp: 71-88Xiong, L.L., R.L. Du, L.L. Xue, Y. Jiang and J. Huang et al.,20202020Roy, N., A. Narayanankutty, P.A. Nazeem, R. Valsalan, T.D. Babu and D. Mathew,20161740194023Luo, Y., C.Z. Wang, R. Sawadogo, J. Yuan and J. Zeng et al.,2021645514561Ganesan, K. and B. Xu,20171401102113Zhu, H. N. Zhao and M. Jiang,202199741749Imran, M., A. Rauf, T. Abu-Izneid, M. Nadeem and M.A. Shariati et al.,20192019