International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2022.1278.1286WangYan WangHaibin SunZhongyi SunHongzhi Ur-RehmanUzair TianJiwei 62022186Background and Objective: Recent years have witnessed the increasing incidence of Intervertebral disc degeneration (IDD), the basic pathological changes of many spinal degenerative diseases. The purpose of this research was to explore the influence of HO-1 on autophagy of nucleus pulposus cells (NPCs) in IDD rats, to provide a new target for IDD treatment. Materials and Methods: About 40 SD rats were randomly divided into four groups: Model, blank, HO-1 and control group. Model group rats were established IDD model only. The blank group was infected with an Adeno-associated virus packaged with an empty vector. HO-1 group rats were infected with HO-1 overexpression Adeno-associated virus vector. Control group rats were fed normally. After modelling, HO-1 mRNA levels, pain threshold and inclined plane test (IPT) score were measured. IL-1β, IL-6, TNF-α, SOD and MDA were detected by ELISA. NPCs were isolated to detect cell multiplication and apoptosis. Beclin-1 and LC-3 in NPCs were determined by western blot. Results: The model and the blank group had similar HO-1 mRNA (p>0.05), pain threshold and IPT scores were lower than the control group and higher than the HO-1 group (p<0.05). IL-1β, IL-6, TNF-α and MDA increased, while SOD decreased (p<0.05). In vitro, the multiplication capacity of NPCs was highest in the control group (p<0.05), Beclin-1 and LC-3 protein expression in blank and model groups were the same (p<0.05) but was higher than control and lower than HO-1 group (p<0.05). Conclusion: Elevation in IDD, HO-1 can exacerbate inflammation and oxidative stress via activating NPC autophagy, which may be the key to future treatment of IDD.]]>Vergroesen, P.P., I. Kingma, K.S. Emanuel, R.J.W. Hoogendoorn and T.J. Welting et al.,20152310571070Risbud, M.V. and I.M. Shapiro,2013104456Kos, N., L. Gradisnik and T. Velnar,201973421424Paul, C.P.L., K.S. Emanuel, I. Kingma, A.J.V. Veen and R.M. Holewijn et al.,20182018Pan, J., X. Lu, G. Yang, Y. Han, X. Tong and Y. Wang,20172017Li, G., Q. Wang, H. Liu and Y. Yang,2019125e1063e1068Thavaneswaran, P. and M. Vandepeer,201384121127Schizas, C., G. Kulik and V. Kosmopoulos,201020306315Li, Z., X. Chen, D. Xu, S. Li, M.T.V. Chan and W.K.K. Wu,20192019Liao, Z., R. Luo, G. Li, Y. Song and S. Zhan et al.,2019in vivo.]]>940844100Cheng, X., G. Zhang, L. Zhang, Y. Hu and K. Zhang et al.,2017via exosomes to inhibit nucleus pulposus cell apoptosis and reduce intervertebral disc degeneration.]]>22261276Che, H., J. Li, Y. Li, C. Ma and H. Liu et al.,20202020Waza, A.A., Z. Hamid, S. Ali, S.A. Bhat and M.A. Bhat,201867579588Bereczki, D., J. Balla and D. Bereczki,20182422292235Vasconcellos, L.R., M.S. Siqueira, R. Moraes, L.A. Carneiro, M.T. Bozza and L.H. Travassos,20182423112316Neis, V.B., P.B. Rosa, M. Moretti and A.L.S. Rodrigues,20182422832302Wu, P.H., H.S. Kim and I.T. Jang,20202020Veraa, S., W. Bergmann, I.D. Wijnberg, W. Back, H. Vernooij, M. Nielen and A.J.M. van den Belt,201960696706Bergknut, N., L.A. Smolders, G.C.M. Grinwis, R. Hagman and A.S. Lagerstedt et al.,2013195282291Zhu, L., C. Yu, X. Zhang, Z. Yu and F. Zhan et al.,20202020Ohta, R., N. Tanaka, K. Nakanishi, N. Kamei and T. Nakamae et al.,20122117481757Adams, M.A. and P. Dolan,2012221497506Hu, B., C. Shi, C. Xu, P. Cao and Y. Tian et al.,20162016Zhang, G.Z., Y.J. Deng, Q.Q. Xie, E.H. Ren and Z.J. Ma et al.,20205083342Basso, M., L. Cavagnaro, A. Zanirato, S. Divano, C. Formica, M. Formica and L. Felli,201710193104Kapoor, M., J. Martel-Pelletier, D. Lajeunesse, J.P. Pelletier and H. Fahmi,201073342Liu, S., L. Tian, G. Chai, B. Wen and B. Wang,2018via inhibiting NLRP3 inflammasome activation.]]>941844193Mangan, M.S.J., E.J. Olhava, W.R. Roush, H.M. Seidel, G.D. Glick and E. Latz,201817588606He, R., M. Cui, H. Lin, L. Zhao, J. Wang, S. Chen and Z. Shao,2018199122130Kang, L., Q. Xiang, S. Zhan, Y. Song and K. Wang et al.,20192019Dong, C., H. Zheng, S. Huang, N. You and J. Xu et al.,2015337146159Wang, D., X. He, D. Wang, P. Peng and X. Xu et al.,20202020Chen, F., H. Liu, X. Wang, Z. Li and J. Zhang et al.,20202811211132