International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2021.180.189XianchuLiu HuanPeng BeiwangDeng ChanghaoCheng MingLiu 42021174Background and Objective: Diabetic amyotrophy is one of serious muscular complications in diabetes. Asiatic Acid (AA) possessed multiple pharmacological actions in diabetic complications. However, the potential role of AA on diabetic myopathy has been not reported to date. Current study aimed to estimate improvement effect of AA on diabetic-associated muscle atrophy in mice. Materials and Methods: Mice were intraperitoneal injection with streptozocin (STZ, 50 mg kg1) for 5 days to establish diabetic model. When blood glucose concentration was above 16.7 mmol/L, mice were treated with AA (30 mg/kg/day) for 8 weeks. Then, mice were euthanized to collect blood and muscle samples for further research. Results: Our data demonstrated that AA significantly reduced blood glucose concentration and led to a significant decrease in serum glucose (Glu), triglyceride (TG), cholesterol (TC), Creatine Kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea (BUN) and creatinine (Cre) in diabetic mice. Moreover, AA significantly increased myofiber size and weight of gastrocnemius and enhanced grip strength to improve muscle atrophy. In skeletal muscle, AA significantly relieved Atrogin-1, MuRF-1, TNF-a, IL-6 and Bax expressions and elevated Nrf-1, Pgc-1α and Bcl-2 expressions in diabetic mice. Conclusion: AA produced beneficial effects against diabetic amyotrophy through its accommodation of inflammatory response, mitochondrial dysfunction and apoptosis in mice. These biological functions suggested AA could be used as a novel medicine for improvement of diabetes-induced skeletal muscle atrophy.<]]>Bluestone, J.A., K. Herold and G. Eisenbarth,201046412931300Burghardt, K., U.A. Müller, N. Müller, C. Kloos, G. Kramer, V. Jörgens and N. Kuniss,2020128796803Mallick, C., R. Maiti and D. Ghosh,2006Eugenia jambolana) and root of kadali (Musa paradisiacal) in streptozotocin-induced diabetic male albino rat: A comparative study.]]>2492503Evans, P.L., S.L. Mcmillin, L.A. Weyrauch and C.A. Witczak,20192019Muramatsu, K., M. Niwa, T. Tamaki, M. Ikutomo and Y. Masu et al.,20171152128Liu, S.H., Y.H. Chang and M.T. Chiang,20105857955800Jurisic-Erzen, D., G. Starcevic-Klasan, D. Ivanac, S. Peharec, D. Girotto and R. Jerkovic,201841203209Tang, L., N. Li, W. Jian, Y. Kang and B. Yin et al.,20172017Krause, M.P., M.C. Riddell and T.J. Hawke,201112345364Rusbana, T.B., A.Z. Agista, W.D. Saputra, Y. Ohsaki and K. Watanabe et al.,20202020Hernández-Ochoa, E.O., P. Llanos and J.T. Lanner,20172017Zebrowska, A., B. Hall, A. Maszczyk, R. Banas and J. Urban,2018144126136Alway, S.E.,2019476363Nguyen, T.T.N., H. Choi and H.S. Jun,20202020Ramachandran, V. and R. Saravanan,2015]]>34884893Guo, S., Q. Chen, Y. Sun and J. Chen,2019125470477Kamble, S.M. and C.R. Patil,201818131141Liu, J., L. Chen and H. Lu,20181410381045Ramachandran, V. and R. Saravanan,201320230236Hung, Y.C., H.T. Yang and M.C. Yin,2015via anti-glycative and anti-coagulatory activities in diabetic mice.]]>629672974Ishida, T., M. Iizuka, Y. Ou, S. Morisawa and A. Hirata et al.,20194211281133Yin, L., X. Chen, N. Li, W. Jia and N. Wang et al.,20212021Sartori, R., V. Romanello and M. Sandri,20212021Khalil, R.,20181088235248Yadav, A., A. Singh, J. Phogat, A. Dahuja and R. Dabur,20212021Bodine, S.C. and L.M. Baehr,2014307E469E484O`neill, B.T., G. Bhardwaj, C.M. Penniman, M.T. Krumpoch and P.A.S. Beltran et al.,201968556570Glenn, M.D. and D. Jabari,202038553564Nomura, T., T. Kawae, H. Kataoka and Y. Ikeda,20182018Wang, D., H. Sun, G. Song, Y. Yang, X. Zou, P. Han and S. Li,20182018Lv, H., Z. Qi, S. Wang, H. Feng, X. Deng and X. Ci,20172017Dong, S.H., Y.W. Liu, F. Wei, H.Z. Tan and Z.D. Han,20178912971309Narasimhulu, C.A. and D.K. Singla,202112403420Mastrocola, R., P. Reffo, F. Penna, C.E. Tomasinelli and G. Boccuzzi et al.,200844584593Webster, J.M., L.J.A.P. Kempen, R.S. Hardy and R.C.J. Langen,20202020Ono, T., S. Takada, S. Kinugawa and H. Tsutsui,201510010521063Han, Y., Y. Jiang, Y. Li, M. Wang and T. Fan et al.,2019761256138Wood, N., S. Straw, M. Scalabrin, L.D. Roberts, K.K. Witte and T.S. Bowen,20212021Fujimaki, S. and T. Kuwabara,20172017Ji, L.L. and D. Yeo,20192019Rendon, D.A.,20062623627Gan, Z., T. Fu, D.P. Kelly and R.B. Vega,201828969980Ye, L., M. Li, Z. Wang, Z. Yang and J. Zhang et al.,20201317471756Xu, M.F., Y.Y. Xiong, J.K. Liu, J.J. Qian, L. Zhu and J. Gao,2012Centella asiatica, attenuates glutamate-induced cognitive deficits in mice and apoptosis in SH-SY5Y cells.]]>33578587Dupont-Versteegden, E.E.,20061274637466Reddy, V.S., S. Jakhotia, P.Y. Reddy and G.B. Reddy,201567291299Cauwels, A., E. Rogge, B. Vandendriessche, S. Shiva and P. Brouckaert,20142014Rizwan, H., S. Pal, S. Sabnam and A. Pal,20202020Li, M., H. Qingxiang, X. Lingli, Z. Mei, L. Dan and L. Yongwang,2019155055Yan, S.L., Z.H. Wang, M.C. Mong, Y.C. Yang and M.C. Yin,2019126192198Reddy, S.S., K. Shruthi, D. Joy and G.B. Reddy,20193067077